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Investigation of Genetic Relationships Between Hanseniaspora Species Found in Grape Musts Revealed Interspecific Hybrids With Dynamic Genome Structures

Hanseniaspora, a predominant yeast genus of grape musts, includes sister species recently reported as fast evolving. The aim of this study was to investigate the genetic relationships between the four most closely related species, at the population level. A multi-locus sequence typing strategy based...

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Autores principales: Saubin, Méline, Devillers, Hugo, Proust, Lucas, Brier, Cathy, Grondin, Cécile, Pradal, Martine, Legras, Jean-Luc, Neuvéglise, Cécile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974558/
https://www.ncbi.nlm.nih.gov/pubmed/32010076
http://dx.doi.org/10.3389/fmicb.2019.02960
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author Saubin, Méline
Devillers, Hugo
Proust, Lucas
Brier, Cathy
Grondin, Cécile
Pradal, Martine
Legras, Jean-Luc
Neuvéglise, Cécile
author_facet Saubin, Méline
Devillers, Hugo
Proust, Lucas
Brier, Cathy
Grondin, Cécile
Pradal, Martine
Legras, Jean-Luc
Neuvéglise, Cécile
author_sort Saubin, Méline
collection PubMed
description Hanseniaspora, a predominant yeast genus of grape musts, includes sister species recently reported as fast evolving. The aim of this study was to investigate the genetic relationships between the four most closely related species, at the population level. A multi-locus sequence typing strategy based on five markers was applied on 107 strains, confirming the clear delineation of species H. uvarum, H. opuntiae, H. guilliermondii, and H. pseudoguilliermondii. Huge variations were observed in the level of intraspecific nucleotide diversity, and differences in heterozygosity between species indicate different life styles. No clear population structure was detected based on geographical or substrate origins. Instead, H. guilliermondii strains clustered into two distinct groups, which may reflect a recent step toward speciation. Interspecific hybrids were detected between H. opuntiae and H. pseudoguilliermondii. Their characterization using flow cytometry, karyotypes and genome sequencing showed different genome structures in different ploidy contexts: allodiploids, allotriploids, and allotetraploids. Subculturing of an allotriploid strain revealed chromosome loss equivalent to one chromosome set, followed by an auto-diploidization event, whereas another auto-diploidized tetraploid showed a segmental duplication. Altogether, these results suggest that Hanseniaspora genomes are not only fast evolving but also highly dynamic.
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spelling pubmed-69745582020-01-31 Investigation of Genetic Relationships Between Hanseniaspora Species Found in Grape Musts Revealed Interspecific Hybrids With Dynamic Genome Structures Saubin, Méline Devillers, Hugo Proust, Lucas Brier, Cathy Grondin, Cécile Pradal, Martine Legras, Jean-Luc Neuvéglise, Cécile Front Microbiol Microbiology Hanseniaspora, a predominant yeast genus of grape musts, includes sister species recently reported as fast evolving. The aim of this study was to investigate the genetic relationships between the four most closely related species, at the population level. A multi-locus sequence typing strategy based on five markers was applied on 107 strains, confirming the clear delineation of species H. uvarum, H. opuntiae, H. guilliermondii, and H. pseudoguilliermondii. Huge variations were observed in the level of intraspecific nucleotide diversity, and differences in heterozygosity between species indicate different life styles. No clear population structure was detected based on geographical or substrate origins. Instead, H. guilliermondii strains clustered into two distinct groups, which may reflect a recent step toward speciation. Interspecific hybrids were detected between H. opuntiae and H. pseudoguilliermondii. Their characterization using flow cytometry, karyotypes and genome sequencing showed different genome structures in different ploidy contexts: allodiploids, allotriploids, and allotetraploids. Subculturing of an allotriploid strain revealed chromosome loss equivalent to one chromosome set, followed by an auto-diploidization event, whereas another auto-diploidized tetraploid showed a segmental duplication. Altogether, these results suggest that Hanseniaspora genomes are not only fast evolving but also highly dynamic. Frontiers Media S.A. 2020-01-15 /pmc/articles/PMC6974558/ /pubmed/32010076 http://dx.doi.org/10.3389/fmicb.2019.02960 Text en Copyright © 2020 Saubin, Devillers, Proust, Brier, Grondin, Pradal, Legras and Neuvéglise. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Saubin, Méline
Devillers, Hugo
Proust, Lucas
Brier, Cathy
Grondin, Cécile
Pradal, Martine
Legras, Jean-Luc
Neuvéglise, Cécile
Investigation of Genetic Relationships Between Hanseniaspora Species Found in Grape Musts Revealed Interspecific Hybrids With Dynamic Genome Structures
title Investigation of Genetic Relationships Between Hanseniaspora Species Found in Grape Musts Revealed Interspecific Hybrids With Dynamic Genome Structures
title_full Investigation of Genetic Relationships Between Hanseniaspora Species Found in Grape Musts Revealed Interspecific Hybrids With Dynamic Genome Structures
title_fullStr Investigation of Genetic Relationships Between Hanseniaspora Species Found in Grape Musts Revealed Interspecific Hybrids With Dynamic Genome Structures
title_full_unstemmed Investigation of Genetic Relationships Between Hanseniaspora Species Found in Grape Musts Revealed Interspecific Hybrids With Dynamic Genome Structures
title_short Investigation of Genetic Relationships Between Hanseniaspora Species Found in Grape Musts Revealed Interspecific Hybrids With Dynamic Genome Structures
title_sort investigation of genetic relationships between hanseniaspora species found in grape musts revealed interspecific hybrids with dynamic genome structures
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974558/
https://www.ncbi.nlm.nih.gov/pubmed/32010076
http://dx.doi.org/10.3389/fmicb.2019.02960
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