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Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces

Fouling distinctly reduces the filtration performance of membranes. A characterization of the fouling in membranes, however, is difficult due to its spatial distribution. Currently applied methods for deposit layer analysis are rather complex or do not offer a spatial resolution. Knowledge of the sp...

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Autores principales: Hartinger, Martin, Napiwotzki, Jonas, Schmid, Eva-Maria, Kurz, Franziska, Kulozik, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974784/
https://www.ncbi.nlm.nih.gov/pubmed/31993341
http://dx.doi.org/10.1016/j.mex.2019.100780
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author Hartinger, Martin
Napiwotzki, Jonas
Schmid, Eva-Maria
Kurz, Franziska
Kulozik, Ulrich
author_facet Hartinger, Martin
Napiwotzki, Jonas
Schmid, Eva-Maria
Kurz, Franziska
Kulozik, Ulrich
author_sort Hartinger, Martin
collection PubMed
description Fouling distinctly reduces the filtration performance of membranes. A characterization of the fouling in membranes, however, is difficult due to its spatial distribution. Currently applied methods for deposit layer analysis are rather complex or do not offer a spatial resolution. Knowledge of the spatial distribution, however, could be used to improve the design of membranes, modules, and spacers. Staining with Coomassie Brilliant Blue, related to the staining of PAGE gels, is a simple method to visualize and analyze the deposited proteins semi-quantitatively. We improved an existing staining technique for protein deposits on membranes by adding a calibration for the semi-quantitative analysis and optimizing the sample handling. The method provides a spatially resolved analysis of deposited proteins up to a concentration of 10 g m(−2). Apart from staining, data processing is described in order to generate false colors or topographic images of deposits. Thus, the paper describes a simple method to assess and visualize the influence of module characteristics such as spacer design on the spatially resolved protein fouling of polymeric and ceramic membranes. Therefore, the method can contribute to the improvement of the module design and processing conditions with regard to the filtration performance. • Visualization of proteinaceous deposits on membranes; • Spatially resolved quantification of proteinaceous deposits.
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spelling pubmed-69747842020-01-28 Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces Hartinger, Martin Napiwotzki, Jonas Schmid, Eva-Maria Kurz, Franziska Kulozik, Ulrich MethodsX Chemistry Fouling distinctly reduces the filtration performance of membranes. A characterization of the fouling in membranes, however, is difficult due to its spatial distribution. Currently applied methods for deposit layer analysis are rather complex or do not offer a spatial resolution. Knowledge of the spatial distribution, however, could be used to improve the design of membranes, modules, and spacers. Staining with Coomassie Brilliant Blue, related to the staining of PAGE gels, is a simple method to visualize and analyze the deposited proteins semi-quantitatively. We improved an existing staining technique for protein deposits on membranes by adding a calibration for the semi-quantitative analysis and optimizing the sample handling. The method provides a spatially resolved analysis of deposited proteins up to a concentration of 10 g m(−2). Apart from staining, data processing is described in order to generate false colors or topographic images of deposits. Thus, the paper describes a simple method to assess and visualize the influence of module characteristics such as spacer design on the spatially resolved protein fouling of polymeric and ceramic membranes. Therefore, the method can contribute to the improvement of the module design and processing conditions with regard to the filtration performance. • Visualization of proteinaceous deposits on membranes; • Spatially resolved quantification of proteinaceous deposits. Elsevier 2019-12-19 /pmc/articles/PMC6974784/ /pubmed/31993341 http://dx.doi.org/10.1016/j.mex.2019.100780 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Chemistry
Hartinger, Martin
Napiwotzki, Jonas
Schmid, Eva-Maria
Kurz, Franziska
Kulozik, Ulrich
Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces
title Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces
title_full Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces
title_fullStr Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces
title_full_unstemmed Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces
title_short Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces
title_sort semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974784/
https://www.ncbi.nlm.nih.gov/pubmed/31993341
http://dx.doi.org/10.1016/j.mex.2019.100780
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