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Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces
Fouling distinctly reduces the filtration performance of membranes. A characterization of the fouling in membranes, however, is difficult due to its spatial distribution. Currently applied methods for deposit layer analysis are rather complex or do not offer a spatial resolution. Knowledge of the sp...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974784/ https://www.ncbi.nlm.nih.gov/pubmed/31993341 http://dx.doi.org/10.1016/j.mex.2019.100780 |
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author | Hartinger, Martin Napiwotzki, Jonas Schmid, Eva-Maria Kurz, Franziska Kulozik, Ulrich |
author_facet | Hartinger, Martin Napiwotzki, Jonas Schmid, Eva-Maria Kurz, Franziska Kulozik, Ulrich |
author_sort | Hartinger, Martin |
collection | PubMed |
description | Fouling distinctly reduces the filtration performance of membranes. A characterization of the fouling in membranes, however, is difficult due to its spatial distribution. Currently applied methods for deposit layer analysis are rather complex or do not offer a spatial resolution. Knowledge of the spatial distribution, however, could be used to improve the design of membranes, modules, and spacers. Staining with Coomassie Brilliant Blue, related to the staining of PAGE gels, is a simple method to visualize and analyze the deposited proteins semi-quantitatively. We improved an existing staining technique for protein deposits on membranes by adding a calibration for the semi-quantitative analysis and optimizing the sample handling. The method provides a spatially resolved analysis of deposited proteins up to a concentration of 10 g m(−2). Apart from staining, data processing is described in order to generate false colors or topographic images of deposits. Thus, the paper describes a simple method to assess and visualize the influence of module characteristics such as spacer design on the spatially resolved protein fouling of polymeric and ceramic membranes. Therefore, the method can contribute to the improvement of the module design and processing conditions with regard to the filtration performance. • Visualization of proteinaceous deposits on membranes; • Spatially resolved quantification of proteinaceous deposits. |
format | Online Article Text |
id | pubmed-6974784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-69747842020-01-28 Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces Hartinger, Martin Napiwotzki, Jonas Schmid, Eva-Maria Kurz, Franziska Kulozik, Ulrich MethodsX Chemistry Fouling distinctly reduces the filtration performance of membranes. A characterization of the fouling in membranes, however, is difficult due to its spatial distribution. Currently applied methods for deposit layer analysis are rather complex or do not offer a spatial resolution. Knowledge of the spatial distribution, however, could be used to improve the design of membranes, modules, and spacers. Staining with Coomassie Brilliant Blue, related to the staining of PAGE gels, is a simple method to visualize and analyze the deposited proteins semi-quantitatively. We improved an existing staining technique for protein deposits on membranes by adding a calibration for the semi-quantitative analysis and optimizing the sample handling. The method provides a spatially resolved analysis of deposited proteins up to a concentration of 10 g m(−2). Apart from staining, data processing is described in order to generate false colors or topographic images of deposits. Thus, the paper describes a simple method to assess and visualize the influence of module characteristics such as spacer design on the spatially resolved protein fouling of polymeric and ceramic membranes. Therefore, the method can contribute to the improvement of the module design and processing conditions with regard to the filtration performance. • Visualization of proteinaceous deposits on membranes; • Spatially resolved quantification of proteinaceous deposits. Elsevier 2019-12-19 /pmc/articles/PMC6974784/ /pubmed/31993341 http://dx.doi.org/10.1016/j.mex.2019.100780 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Chemistry Hartinger, Martin Napiwotzki, Jonas Schmid, Eva-Maria Kurz, Franziska Kulozik, Ulrich Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces |
title | Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces |
title_full | Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces |
title_fullStr | Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces |
title_full_unstemmed | Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces |
title_short | Semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces |
title_sort | semi-quantitative, spatially resolved analysis of protein deposit layers on membrane surfaces |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974784/ https://www.ncbi.nlm.nih.gov/pubmed/31993341 http://dx.doi.org/10.1016/j.mex.2019.100780 |
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