Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells

BACKGROUND: We aimed to investigate the effect of PI3K/Akt signaling pathway on PRAS40Thr246 phosphorylation in gastric cancer cells. METHODS: The study was conducted from April 2017 to January 2018 in Zhongshan Hospital, Xiamen University, Xiamen, China. Gastric cancer cells were divided into three...

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Detalles Bibliográficos
Autores principales: LU, Yizhuo, LI, Lianghui, WU, Guoyang, ZHUO, Huiqin, LIU, Guoyan, CAI, Jianchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974862/
https://www.ncbi.nlm.nih.gov/pubmed/31993387
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author LU, Yizhuo
LI, Lianghui
WU, Guoyang
ZHUO, Huiqin
LIU, Guoyan
CAI, Jianchun
author_facet LU, Yizhuo
LI, Lianghui
WU, Guoyang
ZHUO, Huiqin
LIU, Guoyan
CAI, Jianchun
author_sort LU, Yizhuo
collection PubMed
description BACKGROUND: We aimed to investigate the effect of PI3K/Akt signaling pathway on PRAS40Thr246 phosphorylation in gastric cancer cells. METHODS: The study was conducted from April 2017 to January 2018 in Zhongshan Hospital, Xiamen University, Xiamen, China. Gastric cancer cells were divided into three groups: gastric cancer cell group, LY294002 group and MK-2206 group. Specific tests were conducted accordingly. RESULTS: Inhibition of PI3K/Akt signaling pathway activation and PRAS40Thr246 phosphorylation could inhibit proliferation and invasion and promote apoptosis of gastric cancer cells, and PRAS40Thr246 phosphorylation could activate PI3K/Akt signaling pathway. CONCLUSION: The levels of PI3K/Akt signaling pathway related proteins and p-PRAS40Thr246 were significantly increased in gastric cancer cells. p-PRAS40-Thr246 was able to reflect the activation of the PI3K/Akt signaling pathway, reflecting the sensitivity of the PI3K/AKT signaling pathway to inhibitors.
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spelling pubmed-69748622020-01-28 Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells LU, Yizhuo LI, Lianghui WU, Guoyang ZHUO, Huiqin LIU, Guoyan CAI, Jianchun Iran J Public Health Original Article BACKGROUND: We aimed to investigate the effect of PI3K/Akt signaling pathway on PRAS40Thr246 phosphorylation in gastric cancer cells. METHODS: The study was conducted from April 2017 to January 2018 in Zhongshan Hospital, Xiamen University, Xiamen, China. Gastric cancer cells were divided into three groups: gastric cancer cell group, LY294002 group and MK-2206 group. Specific tests were conducted accordingly. RESULTS: Inhibition of PI3K/Akt signaling pathway activation and PRAS40Thr246 phosphorylation could inhibit proliferation and invasion and promote apoptosis of gastric cancer cells, and PRAS40Thr246 phosphorylation could activate PI3K/Akt signaling pathway. CONCLUSION: The levels of PI3K/Akt signaling pathway related proteins and p-PRAS40Thr246 were significantly increased in gastric cancer cells. p-PRAS40-Thr246 was able to reflect the activation of the PI3K/Akt signaling pathway, reflecting the sensitivity of the PI3K/AKT signaling pathway to inhibitors. Tehran University of Medical Sciences 2019-12 /pmc/articles/PMC6974862/ /pubmed/31993387 Text en Copyright© Iranian Public Health Association & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
LU, Yizhuo
LI, Lianghui
WU, Guoyang
ZHUO, Huiqin
LIU, Guoyan
CAI, Jianchun
Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells
title Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells
title_full Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells
title_fullStr Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells
title_full_unstemmed Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells
title_short Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells
title_sort effect of pi3k/akt signaling pathway on pras40thr246 phosphorylation in gastric cancer cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974862/
https://www.ncbi.nlm.nih.gov/pubmed/31993387
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