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Multiphoton microscopy: a personal historical review, with some future predictions
The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Society of Photo-Optical Instrumentation Engineers
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974959/ https://www.ncbi.nlm.nih.gov/pubmed/31970944 http://dx.doi.org/10.1117/1.JBO.25.1.014511 |
| Sumario: | The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described. |
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