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Multiphoton microscopy: a personal historical review, with some future predictions

The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are...

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Autor principal: Sheppard, Colin J. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974959/
https://www.ncbi.nlm.nih.gov/pubmed/31970944
http://dx.doi.org/10.1117/1.JBO.25.1.014511
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author Sheppard, Colin J. R.
author_facet Sheppard, Colin J. R.
author_sort Sheppard, Colin J. R.
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description The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described.
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spelling pubmed-69749592020-02-03 Multiphoton microscopy: a personal historical review, with some future predictions Sheppard, Colin J. R. J Biomed Opt Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described. Society of Photo-Optical Instrumentation Engineers 2020-01-22 2020-01 /pmc/articles/PMC6974959/ /pubmed/31970944 http://dx.doi.org/10.1117/1.JBO.25.1.014511 Text en © 2020 The Authors https://creativecommons.org/licenses/by/4.0/ Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
Sheppard, Colin J. R.
Multiphoton microscopy: a personal historical review, with some future predictions
title Multiphoton microscopy: a personal historical review, with some future predictions
title_full Multiphoton microscopy: a personal historical review, with some future predictions
title_fullStr Multiphoton microscopy: a personal historical review, with some future predictions
title_full_unstemmed Multiphoton microscopy: a personal historical review, with some future predictions
title_short Multiphoton microscopy: a personal historical review, with some future predictions
title_sort multiphoton microscopy: a personal historical review, with some future predictions
topic Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974959/
https://www.ncbi.nlm.nih.gov/pubmed/31970944
http://dx.doi.org/10.1117/1.JBO.25.1.014511
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