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Multiphoton microscopy: a personal historical review, with some future predictions
The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Society of Photo-Optical Instrumentation Engineers
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974959/ https://www.ncbi.nlm.nih.gov/pubmed/31970944 http://dx.doi.org/10.1117/1.JBO.25.1.014511 |
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author | Sheppard, Colin J. R. |
author_facet | Sheppard, Colin J. R. |
author_sort | Sheppard, Colin J. R. |
collection | PubMed |
description | The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described. |
format | Online Article Text |
id | pubmed-6974959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-69749592020-02-03 Multiphoton microscopy: a personal historical review, with some future predictions Sheppard, Colin J. R. J Biomed Opt Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described. Society of Photo-Optical Instrumentation Engineers 2020-01-22 2020-01 /pmc/articles/PMC6974959/ /pubmed/31970944 http://dx.doi.org/10.1117/1.JBO.25.1.014511 Text en © 2020 The Authors https://creativecommons.org/licenses/by/4.0/ Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences Sheppard, Colin J. R. Multiphoton microscopy: a personal historical review, with some future predictions |
title | Multiphoton microscopy: a personal historical review, with some future predictions |
title_full | Multiphoton microscopy: a personal historical review, with some future predictions |
title_fullStr | Multiphoton microscopy: a personal historical review, with some future predictions |
title_full_unstemmed | Multiphoton microscopy: a personal historical review, with some future predictions |
title_short | Multiphoton microscopy: a personal historical review, with some future predictions |
title_sort | multiphoton microscopy: a personal historical review, with some future predictions |
topic | Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974959/ https://www.ncbi.nlm.nih.gov/pubmed/31970944 http://dx.doi.org/10.1117/1.JBO.25.1.014511 |
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