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Inflammation-related gene expression profiles of salivary extracellular vesicles in patients with head trauma

At present, there is no reliable biomarker for the diagnosis of traumatic brain injury (TBI). Studies have shown that extracellular vesicles released by damaged cells into biological fluids can be used as potential biomarkers for diagnosis of TBI and evaluation of TBI severity. We hypothesize that t...

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Autores principales: Cheng, Yan, Pereira, Mandy, Raukar, Neha P., Reagan, John L., Quesenberry, Mathew, Goldberg, Laura, Borgovan, Theodor, LaFrance Jr, W Curt, Dooner, Mark, Deregibus, Maria, Camussi, Giovanni, Ramratnam, Bharat, Quesenberry, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6975135/
https://www.ncbi.nlm.nih.gov/pubmed/31638091
http://dx.doi.org/10.4103/1673-5374.266924
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author Cheng, Yan
Pereira, Mandy
Raukar, Neha P.
Reagan, John L.
Quesenberry, Mathew
Goldberg, Laura
Borgovan, Theodor
LaFrance Jr, W Curt
Dooner, Mark
Deregibus, Maria
Camussi, Giovanni
Ramratnam, Bharat
Quesenberry, Peter
author_facet Cheng, Yan
Pereira, Mandy
Raukar, Neha P.
Reagan, John L.
Quesenberry, Mathew
Goldberg, Laura
Borgovan, Theodor
LaFrance Jr, W Curt
Dooner, Mark
Deregibus, Maria
Camussi, Giovanni
Ramratnam, Bharat
Quesenberry, Peter
author_sort Cheng, Yan
collection PubMed
description At present, there is no reliable biomarker for the diagnosis of traumatic brain injury (TBI). Studies have shown that extracellular vesicles released by damaged cells into biological fluids can be used as potential biomarkers for diagnosis of TBI and evaluation of TBI severity. We hypothesize that the genetic profile of salivary extracellular vesicles in patients with head trauma differs from that in uninjured subjects. Findings from this hypothesis would help investigate the severity of TBI. This study included 19 subjects, consisting of seven healthy controls who denied history of head trauma, six patients diagnosed with concussion injury from an outpatient concussion clinic, and six patients with TBI who received treatment in the emergency department within 24 hours after injury. Real-time PCR analysis of salivary extracellular vesicles in participants was performed using TaqMan Human Inflammation array. Gene expression analysis revealed nine upregulated genes in emergency department patients (LOX5, ANXA3, CASP1, IL2RG, ITGAM, ITGB2, LTA4H, MAPK14, and TNFRSF1A) and 13 upregulated genes in concussion clinic patients compared with healthy participants (ADRB1, ADRB2, BDKRB1, HRH1, HRH2, LTB4R2, LTB4R, PTAFR, CYSLTR1, CES1, KLK1, MC2R, and PTGER3). Each patient group had a unique profile. Comparison between groups showed that 15 inflammation-related genes had significant expression change. Our results indicate that inflammation biomarkers can be used for diagnosis of TBI and evaluation of disease severity. This study was approved by the Institutional Review Board on December 18, 2015 (approval No. 0078-12) and on June 9, 2016 (approval No. 4093-16).
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spelling pubmed-69751352020-02-03 Inflammation-related gene expression profiles of salivary extracellular vesicles in patients with head trauma Cheng, Yan Pereira, Mandy Raukar, Neha P. Reagan, John L. Quesenberry, Mathew Goldberg, Laura Borgovan, Theodor LaFrance Jr, W Curt Dooner, Mark Deregibus, Maria Camussi, Giovanni Ramratnam, Bharat Quesenberry, Peter Neural Regen Res Research Article At present, there is no reliable biomarker for the diagnosis of traumatic brain injury (TBI). Studies have shown that extracellular vesicles released by damaged cells into biological fluids can be used as potential biomarkers for diagnosis of TBI and evaluation of TBI severity. We hypothesize that the genetic profile of salivary extracellular vesicles in patients with head trauma differs from that in uninjured subjects. Findings from this hypothesis would help investigate the severity of TBI. This study included 19 subjects, consisting of seven healthy controls who denied history of head trauma, six patients diagnosed with concussion injury from an outpatient concussion clinic, and six patients with TBI who received treatment in the emergency department within 24 hours after injury. Real-time PCR analysis of salivary extracellular vesicles in participants was performed using TaqMan Human Inflammation array. Gene expression analysis revealed nine upregulated genes in emergency department patients (LOX5, ANXA3, CASP1, IL2RG, ITGAM, ITGB2, LTA4H, MAPK14, and TNFRSF1A) and 13 upregulated genes in concussion clinic patients compared with healthy participants (ADRB1, ADRB2, BDKRB1, HRH1, HRH2, LTB4R2, LTB4R, PTAFR, CYSLTR1, CES1, KLK1, MC2R, and PTGER3). Each patient group had a unique profile. Comparison between groups showed that 15 inflammation-related genes had significant expression change. Our results indicate that inflammation biomarkers can be used for diagnosis of TBI and evaluation of disease severity. This study was approved by the Institutional Review Board on December 18, 2015 (approval No. 0078-12) and on June 9, 2016 (approval No. 4093-16). Wolters Kluwer - Medknow 2019-10-18 /pmc/articles/PMC6975135/ /pubmed/31638091 http://dx.doi.org/10.4103/1673-5374.266924 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Research Article
Cheng, Yan
Pereira, Mandy
Raukar, Neha P.
Reagan, John L.
Quesenberry, Mathew
Goldberg, Laura
Borgovan, Theodor
LaFrance Jr, W Curt
Dooner, Mark
Deregibus, Maria
Camussi, Giovanni
Ramratnam, Bharat
Quesenberry, Peter
Inflammation-related gene expression profiles of salivary extracellular vesicles in patients with head trauma
title Inflammation-related gene expression profiles of salivary extracellular vesicles in patients with head trauma
title_full Inflammation-related gene expression profiles of salivary extracellular vesicles in patients with head trauma
title_fullStr Inflammation-related gene expression profiles of salivary extracellular vesicles in patients with head trauma
title_full_unstemmed Inflammation-related gene expression profiles of salivary extracellular vesicles in patients with head trauma
title_short Inflammation-related gene expression profiles of salivary extracellular vesicles in patients with head trauma
title_sort inflammation-related gene expression profiles of salivary extracellular vesicles in patients with head trauma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6975135/
https://www.ncbi.nlm.nih.gov/pubmed/31638091
http://dx.doi.org/10.4103/1673-5374.266924
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