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Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy

The aim of this study is to evaluate the osteoblastic collagen synthesis under mechanical stimulation using second-harmonic-generation (SHG) microscopy. We apply SHG microscopy to monitor the collagen fibers synthesized by osteoblast-like cells (MC3T3-E1) without the need for fixation and staining....

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Detalles Bibliográficos
Autores principales: Sato, Katsuya, Matsubara, Oki, Hase, Eiji, Minamikawa, Takeo, Yasui, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6975189/
https://www.ncbi.nlm.nih.gov/pubmed/30635995
http://dx.doi.org/10.1117/1.JBO.24.3.031019

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