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Identification of Loci Through Genome-Wide Association Studies to Improve Tolerance to Sulfur Deficiency in Rice
Sulfur (S) is an essential nutrient for plant growth and development; however, S supply for crop production is decreasing due to reduced inputs from atmospheric deposition and reduced application of S-containing fertilizers. Sulfur deficiency in soil is therefore becoming a widespread cause of reduc...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6975283/ https://www.ncbi.nlm.nih.gov/pubmed/32010158 http://dx.doi.org/10.3389/fpls.2019.01668 |
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author | Pariasca-Tanaka, Juan Baertschi, Cedric Wissuwa, Matthias |
author_facet | Pariasca-Tanaka, Juan Baertschi, Cedric Wissuwa, Matthias |
author_sort | Pariasca-Tanaka, Juan |
collection | PubMed |
description | Sulfur (S) is an essential nutrient for plant growth and development; however, S supply for crop production is decreasing due to reduced inputs from atmospheric deposition and reduced application of S-containing fertilizers. Sulfur deficiency in soil is therefore becoming a widespread cause of reduced grain yield and quality in rice (Oryza sativa L). We therefore assessed the genotypic variation for tolerance to S deficiency in rice and identified loci associated with improved tolerance. Plants were grown in nutrient solution with either low (0.01 mM) or high (1.0 mM) supply of S. Plants grown under low-S treatment showed a reduction in total biomass, mainly due to a marked reduction in shoot biomass, while root biomass and root-to-shoot ratio increased, relative to plants under high-S treatment. Genome-wide association studies (GWAS) identified loci associated with root length (qSUE2-3, qSUE4, and qSUE9), and root (qSUE1, qSUE2-1, and qSUE3-1 and qSUE3-2) or total dry matter (qSUE2, qSUE3-1, and qSUE11). Candidate genes identified at associated loci coded for enzymes involved in secondary S metabolic pathways (sulfotransferases), wherein the sulfated compounds play several roles in plant responses to abiotic stress; cell wall metabolism including wall loosening and modification (carbohydrate hydrolases: beta-glucosidase and beta-gluconase) important for root growth; and cell detoxification (glutathione S-transferase). This study confirmed the existence of genetic variation conferring tolerance to S deficiency among traditional aus rice varieties. The advantageous haplotypes identified could be exploited through marker assisted breeding to improve tolerance to S-deficiency in modern cultivars in order to achieve sustainable crop production and food security. |
format | Online Article Text |
id | pubmed-6975283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69752832020-01-31 Identification of Loci Through Genome-Wide Association Studies to Improve Tolerance to Sulfur Deficiency in Rice Pariasca-Tanaka, Juan Baertschi, Cedric Wissuwa, Matthias Front Plant Sci Plant Science Sulfur (S) is an essential nutrient for plant growth and development; however, S supply for crop production is decreasing due to reduced inputs from atmospheric deposition and reduced application of S-containing fertilizers. Sulfur deficiency in soil is therefore becoming a widespread cause of reduced grain yield and quality in rice (Oryza sativa L). We therefore assessed the genotypic variation for tolerance to S deficiency in rice and identified loci associated with improved tolerance. Plants were grown in nutrient solution with either low (0.01 mM) or high (1.0 mM) supply of S. Plants grown under low-S treatment showed a reduction in total biomass, mainly due to a marked reduction in shoot biomass, while root biomass and root-to-shoot ratio increased, relative to plants under high-S treatment. Genome-wide association studies (GWAS) identified loci associated with root length (qSUE2-3, qSUE4, and qSUE9), and root (qSUE1, qSUE2-1, and qSUE3-1 and qSUE3-2) or total dry matter (qSUE2, qSUE3-1, and qSUE11). Candidate genes identified at associated loci coded for enzymes involved in secondary S metabolic pathways (sulfotransferases), wherein the sulfated compounds play several roles in plant responses to abiotic stress; cell wall metabolism including wall loosening and modification (carbohydrate hydrolases: beta-glucosidase and beta-gluconase) important for root growth; and cell detoxification (glutathione S-transferase). This study confirmed the existence of genetic variation conferring tolerance to S deficiency among traditional aus rice varieties. The advantageous haplotypes identified could be exploited through marker assisted breeding to improve tolerance to S-deficiency in modern cultivars in order to achieve sustainable crop production and food security. Frontiers Media S.A. 2020-01-15 /pmc/articles/PMC6975283/ /pubmed/32010158 http://dx.doi.org/10.3389/fpls.2019.01668 Text en Copyright © 2020 Pariasca-Tanaka, Baertschi and Wissuwa http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Pariasca-Tanaka, Juan Baertschi, Cedric Wissuwa, Matthias Identification of Loci Through Genome-Wide Association Studies to Improve Tolerance to Sulfur Deficiency in Rice |
title | Identification of Loci Through Genome-Wide Association Studies to Improve Tolerance to Sulfur Deficiency in Rice |
title_full | Identification of Loci Through Genome-Wide Association Studies to Improve Tolerance to Sulfur Deficiency in Rice |
title_fullStr | Identification of Loci Through Genome-Wide Association Studies to Improve Tolerance to Sulfur Deficiency in Rice |
title_full_unstemmed | Identification of Loci Through Genome-Wide Association Studies to Improve Tolerance to Sulfur Deficiency in Rice |
title_short | Identification of Loci Through Genome-Wide Association Studies to Improve Tolerance to Sulfur Deficiency in Rice |
title_sort | identification of loci through genome-wide association studies to improve tolerance to sulfur deficiency in rice |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6975283/ https://www.ncbi.nlm.nih.gov/pubmed/32010158 http://dx.doi.org/10.3389/fpls.2019.01668 |
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