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Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish
The epidermis, a multilayered epithelium, surrounds and protects the vertebrate body. It develops from a bilayered epithelium formed of the outer periderm and underlying basal epidermis. How apicobasal polarity is established in the developing epidermis has remained poorly understood. We show that b...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6975926/ https://www.ncbi.nlm.nih.gov/pubmed/31967543 http://dx.doi.org/10.7554/eLife.49064 |
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author | Arora, Prateek Dongre, Shivali Raman, Renuka Sonawane, Mahendra |
author_facet | Arora, Prateek Dongre, Shivali Raman, Renuka Sonawane, Mahendra |
author_sort | Arora, Prateek |
collection | PubMed |
description | The epidermis, a multilayered epithelium, surrounds and protects the vertebrate body. It develops from a bilayered epithelium formed of the outer periderm and underlying basal epidermis. How apicobasal polarity is established in the developing epidermis has remained poorly understood. We show that both the periderm and the basal epidermis exhibit polarised distribution of adherens junctions in zebrafish. aPKC, an apical polarity regulator, maintains the robustness of polarisation of E-cadherin- an adherens junction component- in the periderm. E-cadherin in one layer controls the localisation of E-cadherin in the second layer in a layer non-autonomous manner. Importantly, E-cadherin controls the localisation and levels of Lgl, a basolateral polarity regulator, in a layer autonomous as well non-autonomous manner. Since periderm formation from the enveloping layer precedes the formation of the basal epidermis, our analyses suggest that peridermal polarity, initiated by aPKC, is transduced in a stepwise manner by E-cadherin to the basal layer. |
format | Online Article Text |
id | pubmed-6975926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-69759262020-01-23 Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish Arora, Prateek Dongre, Shivali Raman, Renuka Sonawane, Mahendra eLife Cell Biology The epidermis, a multilayered epithelium, surrounds and protects the vertebrate body. It develops from a bilayered epithelium formed of the outer periderm and underlying basal epidermis. How apicobasal polarity is established in the developing epidermis has remained poorly understood. We show that both the periderm and the basal epidermis exhibit polarised distribution of adherens junctions in zebrafish. aPKC, an apical polarity regulator, maintains the robustness of polarisation of E-cadherin- an adherens junction component- in the periderm. E-cadherin in one layer controls the localisation of E-cadherin in the second layer in a layer non-autonomous manner. Importantly, E-cadherin controls the localisation and levels of Lgl, a basolateral polarity regulator, in a layer autonomous as well non-autonomous manner. Since periderm formation from the enveloping layer precedes the formation of the basal epidermis, our analyses suggest that peridermal polarity, initiated by aPKC, is transduced in a stepwise manner by E-cadherin to the basal layer. eLife Sciences Publications, Ltd 2020-01-22 /pmc/articles/PMC6975926/ /pubmed/31967543 http://dx.doi.org/10.7554/eLife.49064 Text en © 2020, Arora et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Arora, Prateek Dongre, Shivali Raman, Renuka Sonawane, Mahendra Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish |
title | Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish |
title_full | Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish |
title_fullStr | Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish |
title_full_unstemmed | Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish |
title_short | Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish |
title_sort | stepwise polarisation of developing bilayered epidermis is mediated by apkc and e-cadherin in zebrafish |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6975926/ https://www.ncbi.nlm.nih.gov/pubmed/31967543 http://dx.doi.org/10.7554/eLife.49064 |
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