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Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish

The epidermis, a multilayered epithelium, surrounds and protects the vertebrate body. It develops from a bilayered epithelium formed of the outer periderm and underlying basal epidermis. How apicobasal polarity is established in the developing epidermis has remained poorly understood. We show that b...

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Autores principales: Arora, Prateek, Dongre, Shivali, Raman, Renuka, Sonawane, Mahendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6975926/
https://www.ncbi.nlm.nih.gov/pubmed/31967543
http://dx.doi.org/10.7554/eLife.49064
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author Arora, Prateek
Dongre, Shivali
Raman, Renuka
Sonawane, Mahendra
author_facet Arora, Prateek
Dongre, Shivali
Raman, Renuka
Sonawane, Mahendra
author_sort Arora, Prateek
collection PubMed
description The epidermis, a multilayered epithelium, surrounds and protects the vertebrate body. It develops from a bilayered epithelium formed of the outer periderm and underlying basal epidermis. How apicobasal polarity is established in the developing epidermis has remained poorly understood. We show that both the periderm and the basal epidermis exhibit polarised distribution of adherens junctions in zebrafish. aPKC, an apical polarity regulator, maintains the robustness of polarisation of E-cadherin- an adherens junction component- in the periderm. E-cadherin in one layer controls the localisation of E-cadherin in the second layer in a layer non-autonomous manner. Importantly, E-cadherin controls the localisation and levels of Lgl, a basolateral polarity regulator, in a layer autonomous as well non-autonomous manner. Since periderm formation from the enveloping layer precedes the formation of the basal epidermis, our analyses suggest that peridermal polarity, initiated by aPKC, is transduced in a stepwise manner by E-cadherin to the basal layer.
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spelling pubmed-69759262020-01-23 Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish Arora, Prateek Dongre, Shivali Raman, Renuka Sonawane, Mahendra eLife Cell Biology The epidermis, a multilayered epithelium, surrounds and protects the vertebrate body. It develops from a bilayered epithelium formed of the outer periderm and underlying basal epidermis. How apicobasal polarity is established in the developing epidermis has remained poorly understood. We show that both the periderm and the basal epidermis exhibit polarised distribution of adherens junctions in zebrafish. aPKC, an apical polarity regulator, maintains the robustness of polarisation of E-cadherin- an adherens junction component- in the periderm. E-cadherin in one layer controls the localisation of E-cadherin in the second layer in a layer non-autonomous manner. Importantly, E-cadherin controls the localisation and levels of Lgl, a basolateral polarity regulator, in a layer autonomous as well non-autonomous manner. Since periderm formation from the enveloping layer precedes the formation of the basal epidermis, our analyses suggest that peridermal polarity, initiated by aPKC, is transduced in a stepwise manner by E-cadherin to the basal layer. eLife Sciences Publications, Ltd 2020-01-22 /pmc/articles/PMC6975926/ /pubmed/31967543 http://dx.doi.org/10.7554/eLife.49064 Text en © 2020, Arora et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Arora, Prateek
Dongre, Shivali
Raman, Renuka
Sonawane, Mahendra
Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish
title Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish
title_full Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish
title_fullStr Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish
title_full_unstemmed Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish
title_short Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish
title_sort stepwise polarisation of developing bilayered epidermis is mediated by apkc and e-cadherin in zebrafish
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6975926/
https://www.ncbi.nlm.nih.gov/pubmed/31967543
http://dx.doi.org/10.7554/eLife.49064
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