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Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars
Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin. However, the coordination of kinesins remains poorly understood because...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976292/ https://www.ncbi.nlm.nih.gov/pubmed/32010782 http://dx.doi.org/10.1126/sciadv.aax7413 |
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author | Kaneko, Taikopaul Furuta, Ken’ya Oiwa, Kazuhiro Shintaku, Hirofumi Kotera, Hidetoshi Yokokawa, Ryuji |
author_facet | Kaneko, Taikopaul Furuta, Ken’ya Oiwa, Kazuhiro Shintaku, Hirofumi Kotera, Hidetoshi Yokokawa, Ryuji |
author_sort | Kaneko, Taikopaul |
collection | PubMed |
description | Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin. However, the coordination of kinesins remains poorly understood because of the experimental difficulty in controlling the number and arrangement of kinesins, which are considered to affect their coordination. Here, we report that both the number and spacing significantly influence the velocity of microtubules driven by nonprocessive kinesin-14 (Ncd), whereas neither the number nor the spacing changes the velocity in the case of highly processive kinesin-1. This result was realized by the optimum nanopatterning method of kinesins that enables immobilization of a single kinesin on a nanopillar. Our proposed method enables us to study the individual effects of the number and spacing of motors on the collective dynamics of multiple motors. |
format | Online Article Text |
id | pubmed-6976292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-69762922020-01-31 Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars Kaneko, Taikopaul Furuta, Ken’ya Oiwa, Kazuhiro Shintaku, Hirofumi Kotera, Hidetoshi Yokokawa, Ryuji Sci Adv Research Articles Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin. However, the coordination of kinesins remains poorly understood because of the experimental difficulty in controlling the number and arrangement of kinesins, which are considered to affect their coordination. Here, we report that both the number and spacing significantly influence the velocity of microtubules driven by nonprocessive kinesin-14 (Ncd), whereas neither the number nor the spacing changes the velocity in the case of highly processive kinesin-1. This result was realized by the optimum nanopatterning method of kinesins that enables immobilization of a single kinesin on a nanopillar. Our proposed method enables us to study the individual effects of the number and spacing of motors on the collective dynamics of multiple motors. American Association for the Advancement of Science 2020-01-22 /pmc/articles/PMC6976292/ /pubmed/32010782 http://dx.doi.org/10.1126/sciadv.aax7413 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Kaneko, Taikopaul Furuta, Ken’ya Oiwa, Kazuhiro Shintaku, Hirofumi Kotera, Hidetoshi Yokokawa, Ryuji Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars |
title | Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars |
title_full | Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars |
title_fullStr | Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars |
title_full_unstemmed | Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars |
title_short | Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars |
title_sort | different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976292/ https://www.ncbi.nlm.nih.gov/pubmed/32010782 http://dx.doi.org/10.1126/sciadv.aax7413 |
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