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Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars

Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin. However, the coordination of kinesins remains poorly understood because...

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Autores principales: Kaneko, Taikopaul, Furuta, Ken’ya, Oiwa, Kazuhiro, Shintaku, Hirofumi, Kotera, Hidetoshi, Yokokawa, Ryuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976292/
https://www.ncbi.nlm.nih.gov/pubmed/32010782
http://dx.doi.org/10.1126/sciadv.aax7413
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author Kaneko, Taikopaul
Furuta, Ken’ya
Oiwa, Kazuhiro
Shintaku, Hirofumi
Kotera, Hidetoshi
Yokokawa, Ryuji
author_facet Kaneko, Taikopaul
Furuta, Ken’ya
Oiwa, Kazuhiro
Shintaku, Hirofumi
Kotera, Hidetoshi
Yokokawa, Ryuji
author_sort Kaneko, Taikopaul
collection PubMed
description Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin. However, the coordination of kinesins remains poorly understood because of the experimental difficulty in controlling the number and arrangement of kinesins, which are considered to affect their coordination. Here, we report that both the number and spacing significantly influence the velocity of microtubules driven by nonprocessive kinesin-14 (Ncd), whereas neither the number nor the spacing changes the velocity in the case of highly processive kinesin-1. This result was realized by the optimum nanopatterning method of kinesins that enables immobilization of a single kinesin on a nanopillar. Our proposed method enables us to study the individual effects of the number and spacing of motors on the collective dynamics of multiple motors.
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spelling pubmed-69762922020-01-31 Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars Kaneko, Taikopaul Furuta, Ken’ya Oiwa, Kazuhiro Shintaku, Hirofumi Kotera, Hidetoshi Yokokawa, Ryuji Sci Adv Research Articles Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin. However, the coordination of kinesins remains poorly understood because of the experimental difficulty in controlling the number and arrangement of kinesins, which are considered to affect their coordination. Here, we report that both the number and spacing significantly influence the velocity of microtubules driven by nonprocessive kinesin-14 (Ncd), whereas neither the number nor the spacing changes the velocity in the case of highly processive kinesin-1. This result was realized by the optimum nanopatterning method of kinesins that enables immobilization of a single kinesin on a nanopillar. Our proposed method enables us to study the individual effects of the number and spacing of motors on the collective dynamics of multiple motors. American Association for the Advancement of Science 2020-01-22 /pmc/articles/PMC6976292/ /pubmed/32010782 http://dx.doi.org/10.1126/sciadv.aax7413 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Kaneko, Taikopaul
Furuta, Ken’ya
Oiwa, Kazuhiro
Shintaku, Hirofumi
Kotera, Hidetoshi
Yokokawa, Ryuji
Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars
title Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars
title_full Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars
title_fullStr Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars
title_full_unstemmed Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars
title_short Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars
title_sort different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976292/
https://www.ncbi.nlm.nih.gov/pubmed/32010782
http://dx.doi.org/10.1126/sciadv.aax7413
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