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3D printed mold leachates in PDMS microfluidic devices

The introduction of poly(dimethylsiloxane) (PDMS) and soft lithography in the 90’s has revolutionized the field of microfluidics by almost eliminating the need for a clean-room environment for device fabrication. More recently, 3D printing has been introduced to fabricate molds for soft lithography,...

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Autores principales: de Almeida Monteiro Melo Ferraz, Marcia, Nagashima, Jennifer Beth, Venzac, Bastien, Le Gac, Séverine, Songsasen, Nucharin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976631/
https://www.ncbi.nlm.nih.gov/pubmed/31969661
http://dx.doi.org/10.1038/s41598-020-57816-y
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author de Almeida Monteiro Melo Ferraz, Marcia
Nagashima, Jennifer Beth
Venzac, Bastien
Le Gac, Séverine
Songsasen, Nucharin
author_facet de Almeida Monteiro Melo Ferraz, Marcia
Nagashima, Jennifer Beth
Venzac, Bastien
Le Gac, Séverine
Songsasen, Nucharin
author_sort de Almeida Monteiro Melo Ferraz, Marcia
collection PubMed
description The introduction of poly(dimethylsiloxane) (PDMS) and soft lithography in the 90’s has revolutionized the field of microfluidics by almost eliminating the need for a clean-room environment for device fabrication. More recently, 3D printing has been introduced to fabricate molds for soft lithography, the only step for which a clean-room environment is still often necessary, to further support the rapid prototyping of PDMS microfluidic devices. However, toxicity of most of the commercial 3D printing resins has been established, and little is known regarding the potential for 3D printed molds to leak components into the PDMS that would, in turn, hamper cells and/or tissues cultured in the devices. In the present study, we investigated if 3D printed molds produced by stereolithography can leach components into PDMS, and compared 3D printed molds to their more conventional SU-8 counterparts. Different leachates were detected in aqueous solutions incubated in the resulting PDMS devices prepared from widely used PDMS pre-polymer:curing agent ratios (10:1, 15:1 and 20:1), and these leachates were identified as originating from resins and catalyst substances. Next, we explored the possibility to culture cells and tissues in these PDMS devices produced from 3D printed molds and after proper device washing and conditioning. Importantly, we demonstrated that the resulting PDMS devices supported physiological cultures of HeLa cells and ovarian tissues in vitro, with superior outcomes than static conventional cultures.
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spelling pubmed-69766312020-01-29 3D printed mold leachates in PDMS microfluidic devices de Almeida Monteiro Melo Ferraz, Marcia Nagashima, Jennifer Beth Venzac, Bastien Le Gac, Séverine Songsasen, Nucharin Sci Rep Article The introduction of poly(dimethylsiloxane) (PDMS) and soft lithography in the 90’s has revolutionized the field of microfluidics by almost eliminating the need for a clean-room environment for device fabrication. More recently, 3D printing has been introduced to fabricate molds for soft lithography, the only step for which a clean-room environment is still often necessary, to further support the rapid prototyping of PDMS microfluidic devices. However, toxicity of most of the commercial 3D printing resins has been established, and little is known regarding the potential for 3D printed molds to leak components into the PDMS that would, in turn, hamper cells and/or tissues cultured in the devices. In the present study, we investigated if 3D printed molds produced by stereolithography can leach components into PDMS, and compared 3D printed molds to their more conventional SU-8 counterparts. Different leachates were detected in aqueous solutions incubated in the resulting PDMS devices prepared from widely used PDMS pre-polymer:curing agent ratios (10:1, 15:1 and 20:1), and these leachates were identified as originating from resins and catalyst substances. Next, we explored the possibility to culture cells and tissues in these PDMS devices produced from 3D printed molds and after proper device washing and conditioning. Importantly, we demonstrated that the resulting PDMS devices supported physiological cultures of HeLa cells and ovarian tissues in vitro, with superior outcomes than static conventional cultures. Nature Publishing Group UK 2020-01-22 /pmc/articles/PMC6976631/ /pubmed/31969661 http://dx.doi.org/10.1038/s41598-020-57816-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
de Almeida Monteiro Melo Ferraz, Marcia
Nagashima, Jennifer Beth
Venzac, Bastien
Le Gac, Séverine
Songsasen, Nucharin
3D printed mold leachates in PDMS microfluidic devices
title 3D printed mold leachates in PDMS microfluidic devices
title_full 3D printed mold leachates in PDMS microfluidic devices
title_fullStr 3D printed mold leachates in PDMS microfluidic devices
title_full_unstemmed 3D printed mold leachates in PDMS microfluidic devices
title_short 3D printed mold leachates in PDMS microfluidic devices
title_sort 3d printed mold leachates in pdms microfluidic devices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976631/
https://www.ncbi.nlm.nih.gov/pubmed/31969661
http://dx.doi.org/10.1038/s41598-020-57816-y
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