The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis

The cytokinesis-block micronucleus (CBMN) assay is considered to be the most suitable biodosimetry method for automation. Previously, we automated this assay on a commercial robotic biotech high-throughput system (RABiT-II) adopting both a traditional and an accelerated micronucleus protocol, using...

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Autores principales: Repin, Mikhail, Pampou, Sergey, Brenner, David J, Garty, Guy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976732/
https://www.ncbi.nlm.nih.gov/pubmed/31825079
http://dx.doi.org/10.1093/jrr/rrz074
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author Repin, Mikhail
Pampou, Sergey
Brenner, David J
Garty, Guy
author_facet Repin, Mikhail
Pampou, Sergey
Brenner, David J
Garty, Guy
author_sort Repin, Mikhail
collection PubMed
description The cytokinesis-block micronucleus (CBMN) assay is considered to be the most suitable biodosimetry method for automation. Previously, we automated this assay on a commercial robotic biotech high-throughput system (RABiT-II) adopting both a traditional and an accelerated micronucleus protocol, using centrifugation steps for both lymphocyte harvesting and washing, after whole blood culturing. Here we describe further development of our accelerated CBMN assay protocol for use on high-throughput/high content screening (HTS/HCS) robotic systems without a centrifuge. This opens the way for implementation of the CBMN assay on a wider range of commercial automated HTS/HCS systems and thus increases the potential capacity for dose estimates following a mass-casualty radiological event.
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spelling pubmed-69767322020-01-27 The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis Repin, Mikhail Pampou, Sergey Brenner, David J Garty, Guy J Radiat Res Letter to the Editor The cytokinesis-block micronucleus (CBMN) assay is considered to be the most suitable biodosimetry method for automation. Previously, we automated this assay on a commercial robotic biotech high-throughput system (RABiT-II) adopting both a traditional and an accelerated micronucleus protocol, using centrifugation steps for both lymphocyte harvesting and washing, after whole blood culturing. Here we describe further development of our accelerated CBMN assay protocol for use on high-throughput/high content screening (HTS/HCS) robotic systems without a centrifuge. This opens the way for implementation of the CBMN assay on a wider range of commercial automated HTS/HCS systems and thus increases the potential capacity for dose estimates following a mass-casualty radiological event. Oxford University Press 2019-12-11 /pmc/articles/PMC6976732/ /pubmed/31825079 http://dx.doi.org/10.1093/jrr/rrz074 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Letter to the Editor
Repin, Mikhail
Pampou, Sergey
Brenner, David J
Garty, Guy
The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
title The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
title_full The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
title_fullStr The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
title_full_unstemmed The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
title_short The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
title_sort use of a centrifuge-free rabit-ii system for high-throughput micronucleus analysis
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976732/
https://www.ncbi.nlm.nih.gov/pubmed/31825079
http://dx.doi.org/10.1093/jrr/rrz074
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