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Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen
OBJECTIVE: Immunocytochemistry (ICC) of serous effusion is an important tool for the diagnosis of benign and malignant cells. Our aim was to develop a modified liquid-based cytological technique for ICC (i.e., a modified LBC). METHODS: Serous effusions of 110 cases were collected for cytological exa...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
West Asia Organization for Cancer Prevention
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976855/ https://www.ncbi.nlm.nih.gov/pubmed/31554354 http://dx.doi.org/10.31557/APJCP.2019.20.9.2611 |
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author | Patarapadungkit, Natcha Jangsiriwitayakorn, Porntip Chaiwiriyakul, Surachat Sirivech, Phannatorn Thongbor, Ratchaneekorn Phanomsri, Em-Orn Nititarakul, Luxkana |
author_facet | Patarapadungkit, Natcha Jangsiriwitayakorn, Porntip Chaiwiriyakul, Surachat Sirivech, Phannatorn Thongbor, Ratchaneekorn Phanomsri, Em-Orn Nititarakul, Luxkana |
author_sort | Patarapadungkit, Natcha |
collection | PubMed |
description | OBJECTIVE: Immunocytochemistry (ICC) of serous effusion is an important tool for the diagnosis of benign and malignant cells. Our aim was to develop a modified liquid-based cytological technique for ICC (i.e., a modified LBC). METHODS: Serous effusions of 110 cases were collected for cytological examination: 50 were negative for malignancy albeit benign mesothelium was found, and 60 were confirmed metastatic adenocarcinoma according to the modified LBC preparation. The latter were stained for EMA, Ber-EP4, Calretinin, and p63 then interpreted by both a cytotechnologist and a pathologist. A comparative analysis of the diagnostic results was conducted. RESULTS: The results of the metastatic adenocarcinoma were 100% (60/60) positive for EMA and 91.7% (55/60) positive for Ber-Ep4 but negative for calretinin and p63. Cases negative for malignancy were 100% (50/50) positive for calretinin but negative for carcinoma markers. The difference between ‘positive for metastatic adenocarcinoma’ and ‘negative for malignancy’ in ICC was statistically significant (p < 0.001). CONCLUSION: The current study demonstrated that a panel marker, comprising EMA, Ber-EP4, and calretinin can be used for differentiating between cases of metastatic adenocarcinoma and benign mesothelium. The serous effusion specimen collected by the modified LBC technique is an effective preparation method for ICC. |
format | Online Article Text |
id | pubmed-6976855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-69768552020-02-04 Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen Patarapadungkit, Natcha Jangsiriwitayakorn, Porntip Chaiwiriyakul, Surachat Sirivech, Phannatorn Thongbor, Ratchaneekorn Phanomsri, Em-Orn Nititarakul, Luxkana Asian Pac J Cancer Prev Research Article OBJECTIVE: Immunocytochemistry (ICC) of serous effusion is an important tool for the diagnosis of benign and malignant cells. Our aim was to develop a modified liquid-based cytological technique for ICC (i.e., a modified LBC). METHODS: Serous effusions of 110 cases were collected for cytological examination: 50 were negative for malignancy albeit benign mesothelium was found, and 60 were confirmed metastatic adenocarcinoma according to the modified LBC preparation. The latter were stained for EMA, Ber-EP4, Calretinin, and p63 then interpreted by both a cytotechnologist and a pathologist. A comparative analysis of the diagnostic results was conducted. RESULTS: The results of the metastatic adenocarcinoma were 100% (60/60) positive for EMA and 91.7% (55/60) positive for Ber-Ep4 but negative for calretinin and p63. Cases negative for malignancy were 100% (50/50) positive for calretinin but negative for carcinoma markers. The difference between ‘positive for metastatic adenocarcinoma’ and ‘negative for malignancy’ in ICC was statistically significant (p < 0.001). CONCLUSION: The current study demonstrated that a panel marker, comprising EMA, Ber-EP4, and calretinin can be used for differentiating between cases of metastatic adenocarcinoma and benign mesothelium. The serous effusion specimen collected by the modified LBC technique is an effective preparation method for ICC. West Asia Organization for Cancer Prevention 2019 /pmc/articles/PMC6976855/ /pubmed/31554354 http://dx.doi.org/10.31557/APJCP.2019.20.9.2611 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Patarapadungkit, Natcha Jangsiriwitayakorn, Porntip Chaiwiriyakul, Surachat Sirivech, Phannatorn Thongbor, Ratchaneekorn Phanomsri, Em-Orn Nititarakul, Luxkana Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen |
title | Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen |
title_full | Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen |
title_fullStr | Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen |
title_full_unstemmed | Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen |
title_short | Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen |
title_sort | modified liquid-based cytology technique for immunocytochemistry in effusion specimen |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976855/ https://www.ncbi.nlm.nih.gov/pubmed/31554354 http://dx.doi.org/10.31557/APJCP.2019.20.9.2611 |
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