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Seventeen new microsatellites for Tamarix gallica and cross‐amplification in Tamarix species

PREMISE: Microsatellite markers were developed for the western Mediterranean tree Tamarix gallica (Tamaricaceae) as part of a study of its genetic diversity and structure. METHODS AND RESULTS: Seventeen microsatellite markers were developed for T. gallica, 14 of which were polymorphic. These microsa...

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Autores principales: Terrones, Alejandro, Juan, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976893/
https://www.ncbi.nlm.nih.gov/pubmed/31993259
http://dx.doi.org/10.1002/aps3.11317
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author Terrones, Alejandro
Juan, Ana
author_facet Terrones, Alejandro
Juan, Ana
author_sort Terrones, Alejandro
collection PubMed
description PREMISE: Microsatellite markers were developed for the western Mediterranean tree Tamarix gallica (Tamaricaceae) as part of a study of its genetic diversity and structure. METHODS AND RESULTS: Seventeen microsatellite markers were developed for T. gallica, 14 of which were polymorphic. These microsatellites have di‐, tri‐, and tetranucleotide repeats with 1–13 alleles per locus and population. Levels of observed and expected heterozygosity ranged from 0.000 to 0.900 and from 0.000 to 0.863, respectively. Six microsatellites showed significant deviations from Hardy–Weinberg equilibrium in at least one population. Cross‐amplification in 19 Tamarix species showed a wide transferability to other species of the genus. CONCLUSIONS: The 14 new polymorphic microsatellite markers will be used to assess the genetic diversity and population genetic structure of T. gallica. Additionally, the successful cross‐species amplification suggests their potential usefulness for investigating species delimitation and population genetics in the genus Tamarix.
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spelling pubmed-69768932020-01-28 Seventeen new microsatellites for Tamarix gallica and cross‐amplification in Tamarix species Terrones, Alejandro Juan, Ana Appl Plant Sci Primer Notes PREMISE: Microsatellite markers were developed for the western Mediterranean tree Tamarix gallica (Tamaricaceae) as part of a study of its genetic diversity and structure. METHODS AND RESULTS: Seventeen microsatellite markers were developed for T. gallica, 14 of which were polymorphic. These microsatellites have di‐, tri‐, and tetranucleotide repeats with 1–13 alleles per locus and population. Levels of observed and expected heterozygosity ranged from 0.000 to 0.900 and from 0.000 to 0.863, respectively. Six microsatellites showed significant deviations from Hardy–Weinberg equilibrium in at least one population. Cross‐amplification in 19 Tamarix species showed a wide transferability to other species of the genus. CONCLUSIONS: The 14 new polymorphic microsatellite markers will be used to assess the genetic diversity and population genetic structure of T. gallica. Additionally, the successful cross‐species amplification suggests their potential usefulness for investigating species delimitation and population genetics in the genus Tamarix. John Wiley and Sons Inc. 2020-01-22 /pmc/articles/PMC6976893/ /pubmed/31993259 http://dx.doi.org/10.1002/aps3.11317 Text en © 2020 Terrones and Juan. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Primer Notes
Terrones, Alejandro
Juan, Ana
Seventeen new microsatellites for Tamarix gallica and cross‐amplification in Tamarix species
title Seventeen new microsatellites for Tamarix gallica and cross‐amplification in Tamarix species
title_full Seventeen new microsatellites for Tamarix gallica and cross‐amplification in Tamarix species
title_fullStr Seventeen new microsatellites for Tamarix gallica and cross‐amplification in Tamarix species
title_full_unstemmed Seventeen new microsatellites for Tamarix gallica and cross‐amplification in Tamarix species
title_short Seventeen new microsatellites for Tamarix gallica and cross‐amplification in Tamarix species
title_sort seventeen new microsatellites for tamarix gallica and cross‐amplification in tamarix species
topic Primer Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976893/
https://www.ncbi.nlm.nih.gov/pubmed/31993259
http://dx.doi.org/10.1002/aps3.11317
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