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Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis

The Asian Buffalo leech, Hirudinaria manillensis, is an aquatic sanguivorous species distributed widely in Southeast Asia. H. manillensis has long been used clinically for bloodletting and other medical purposes. Recent studies have focused on artificial culturing, strain optimization, and the ident...

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Autores principales: Guan, De-Long, Yang, Jie, Liu, Ying-Kui, Li, Yuan, Mi, Da, Ma, Li-Bin, Wang, Zhe-Zhi, Xu, Sheng-Quan, Qiu, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977106/
https://www.ncbi.nlm.nih.gov/pubmed/32010187
http://dx.doi.org/10.3389/fgene.2019.01321
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author Guan, De-Long
Yang, Jie
Liu, Ying-Kui
Li, Yuan
Mi, Da
Ma, Li-Bin
Wang, Zhe-Zhi
Xu, Sheng-Quan
Qiu, Qiang
author_facet Guan, De-Long
Yang, Jie
Liu, Ying-Kui
Li, Yuan
Mi, Da
Ma, Li-Bin
Wang, Zhe-Zhi
Xu, Sheng-Quan
Qiu, Qiang
author_sort Guan, De-Long
collection PubMed
description The Asian Buffalo leech, Hirudinaria manillensis, is an aquatic sanguivorous species distributed widely in Southeast Asia. H. manillensis has long been used clinically for bloodletting and other medical purposes. Recent studies have focused on artificial culturing, strain optimization, and the identification and development new drugs based on the anticoagulant effects of H. manillensis bites; however, data regarding its genome remain unclear. This study aimed to determine the genome sequence of an adult Asian Buffalo leech. We generated a draft assembly of 151.8 Mb and a N50 scaffold of 2.28 Mb. Predictions indicated that the assembled genome contained 21,005 protein-coding genes. Up to 17,865 genes were annotated in multiple databases including Gene Ontology. Sixteen anticoagulant proteins with a Hirudin or Antistasin domain were identified. This study is the first to report the whole-genome sequence of the Asian Buffalo leech, an important sanguivorous leech of clinical significance. The quality of the assembly is comparable to those of other annelids. These data will help further the current understanding of the biological mechanisms and genetic characteristics of leeches and serve as a valuable resource for future studies.
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spelling pubmed-69771062020-02-01 Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis Guan, De-Long Yang, Jie Liu, Ying-Kui Li, Yuan Mi, Da Ma, Li-Bin Wang, Zhe-Zhi Xu, Sheng-Quan Qiu, Qiang Front Genet Genetics The Asian Buffalo leech, Hirudinaria manillensis, is an aquatic sanguivorous species distributed widely in Southeast Asia. H. manillensis has long been used clinically for bloodletting and other medical purposes. Recent studies have focused on artificial culturing, strain optimization, and the identification and development new drugs based on the anticoagulant effects of H. manillensis bites; however, data regarding its genome remain unclear. This study aimed to determine the genome sequence of an adult Asian Buffalo leech. We generated a draft assembly of 151.8 Mb and a N50 scaffold of 2.28 Mb. Predictions indicated that the assembled genome contained 21,005 protein-coding genes. Up to 17,865 genes were annotated in multiple databases including Gene Ontology. Sixteen anticoagulant proteins with a Hirudin or Antistasin domain were identified. This study is the first to report the whole-genome sequence of the Asian Buffalo leech, an important sanguivorous leech of clinical significance. The quality of the assembly is comparable to those of other annelids. These data will help further the current understanding of the biological mechanisms and genetic characteristics of leeches and serve as a valuable resource for future studies. Frontiers Media S.A. 2020-01-16 /pmc/articles/PMC6977106/ /pubmed/32010187 http://dx.doi.org/10.3389/fgene.2019.01321 Text en Copyright © 2020 Guan, Yang, Liu, Li, Mi, Ma, Wang, Xu and Qiu http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Guan, De-Long
Yang, Jie
Liu, Ying-Kui
Li, Yuan
Mi, Da
Ma, Li-Bin
Wang, Zhe-Zhi
Xu, Sheng-Quan
Qiu, Qiang
Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis
title Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis
title_full Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis
title_fullStr Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis
title_full_unstemmed Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis
title_short Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis
title_sort draft genome of the asian buffalo leech hirudinaria manillensis
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977106/
https://www.ncbi.nlm.nih.gov/pubmed/32010187
http://dx.doi.org/10.3389/fgene.2019.01321
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