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Candida auris Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin
Emerging pathogen Candida auris causes nosocomial outbreaks of life-threatening invasive candidiasis. It is unclear how this species colonizes skin and spreads in health care facilities. Here, we analyzed C. auris growth in synthetic sweat medium designed to mimic axillary skin conditions. We show t...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977180/ https://www.ncbi.nlm.nih.gov/pubmed/31969479 http://dx.doi.org/10.1128/mSphere.00910-19 |
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author | Horton, Mark V. Johnson, Chad J. Kernien, John F. Patel, Tarika D. Lam, Brandon C. Cheong, J. Z. Alex Meudt, Jennifer J. Shanmuganayagam, Dhanansayan Kalan, Lindsay R. Nett, Jeniel E. |
author_facet | Horton, Mark V. Johnson, Chad J. Kernien, John F. Patel, Tarika D. Lam, Brandon C. Cheong, J. Z. Alex Meudt, Jennifer J. Shanmuganayagam, Dhanansayan Kalan, Lindsay R. Nett, Jeniel E. |
author_sort | Horton, Mark V. |
collection | PubMed |
description | Emerging pathogen Candida auris causes nosocomial outbreaks of life-threatening invasive candidiasis. It is unclear how this species colonizes skin and spreads in health care facilities. Here, we analyzed C. auris growth in synthetic sweat medium designed to mimic axillary skin conditions. We show that C. auris demonstrates a high capacity for biofilm formation in this milieu, well beyond that observed for the most commonly isolated Candida sp., Candida albicans. The C. auris biofilms persist in environmental conditions expected in the hospital setting. To model C. auris skin colonization, we designed an ex vivo porcine skin model. We show that C. auris proliferates on porcine skin in multilayer biofilms. This capacity to thrive in skin niche conditions helps explain the propensity of C. auris to colonize skin, persist on medical devices, and rapidly spread in hospitals. These studies provide clinically relevant tools to further characterize this important growth modality. IMPORTANCE The emerging fungal pathogen Candida auris causes invasive infections and is spreading in hospitals worldwide. Why this species exhibits the capacity to transfer efficiently among patients is unknown. Our findings reveal that C. auris forms high-burden biofilms in conditions mimicking sweat on the skin surface. These adherent biofilm communities persist in environmental conditions expected in the hospital setting. Using a pig skin model, we show that C. auris also forms high-burden biofilm structures on the skin surface. Identification of this mode of growth sheds light on how this recently described pathogen persists in hospital settings and spreads among patients. |
format | Online Article Text |
id | pubmed-6977180 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-69771802020-02-04 Candida auris Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin Horton, Mark V. Johnson, Chad J. Kernien, John F. Patel, Tarika D. Lam, Brandon C. Cheong, J. Z. Alex Meudt, Jennifer J. Shanmuganayagam, Dhanansayan Kalan, Lindsay R. Nett, Jeniel E. mSphere Research Article Emerging pathogen Candida auris causes nosocomial outbreaks of life-threatening invasive candidiasis. It is unclear how this species colonizes skin and spreads in health care facilities. Here, we analyzed C. auris growth in synthetic sweat medium designed to mimic axillary skin conditions. We show that C. auris demonstrates a high capacity for biofilm formation in this milieu, well beyond that observed for the most commonly isolated Candida sp., Candida albicans. The C. auris biofilms persist in environmental conditions expected in the hospital setting. To model C. auris skin colonization, we designed an ex vivo porcine skin model. We show that C. auris proliferates on porcine skin in multilayer biofilms. This capacity to thrive in skin niche conditions helps explain the propensity of C. auris to colonize skin, persist on medical devices, and rapidly spread in hospitals. These studies provide clinically relevant tools to further characterize this important growth modality. IMPORTANCE The emerging fungal pathogen Candida auris causes invasive infections and is spreading in hospitals worldwide. Why this species exhibits the capacity to transfer efficiently among patients is unknown. Our findings reveal that C. auris forms high-burden biofilms in conditions mimicking sweat on the skin surface. These adherent biofilm communities persist in environmental conditions expected in the hospital setting. Using a pig skin model, we show that C. auris also forms high-burden biofilm structures on the skin surface. Identification of this mode of growth sheds light on how this recently described pathogen persists in hospital settings and spreads among patients. American Society for Microbiology 2020-01-15 /pmc/articles/PMC6977180/ /pubmed/31969479 http://dx.doi.org/10.1128/mSphere.00910-19 Text en Copyright © 2020 Horton et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Horton, Mark V. Johnson, Chad J. Kernien, John F. Patel, Tarika D. Lam, Brandon C. Cheong, J. Z. Alex Meudt, Jennifer J. Shanmuganayagam, Dhanansayan Kalan, Lindsay R. Nett, Jeniel E. Candida auris Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin |
title | Candida auris Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin |
title_full | Candida auris Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin |
title_fullStr | Candida auris Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin |
title_full_unstemmed | Candida auris Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin |
title_short | Candida auris Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin |
title_sort | candida auris forms high-burden biofilms in skin niche conditions and on porcine skin |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977180/ https://www.ncbi.nlm.nih.gov/pubmed/31969479 http://dx.doi.org/10.1128/mSphere.00910-19 |
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