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Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers

Genetic mapping is a basic tool for eukaryotic genomic research. It allows the localization of genes or quantitative trait loci (QTLs) and map-based cloning. In this study, we constructed a linkage map based on DNA samples from a commercial strain L808, including two parental monokaryons and 93 sing...

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Autores principales: Dong, Hui, Shang, Xiaodong, Zhao, Xiaoyan, Yu, Hailong, Jiang, Ning, Zhang, Meiyan, Tan, Qi, Zhou, Changyan, Zhang, Lujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977451/
https://www.ncbi.nlm.nih.gov/pubmed/31988622
http://dx.doi.org/10.1270/jsbbs.18123
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author Dong, Hui
Shang, Xiaodong
Zhao, Xiaoyan
Yu, Hailong
Jiang, Ning
Zhang, Meiyan
Tan, Qi
Zhou, Changyan
Zhang, Lujun
author_facet Dong, Hui
Shang, Xiaodong
Zhao, Xiaoyan
Yu, Hailong
Jiang, Ning
Zhang, Meiyan
Tan, Qi
Zhou, Changyan
Zhang, Lujun
author_sort Dong, Hui
collection PubMed
description Genetic mapping is a basic tool for eukaryotic genomic research. It allows the localization of genes or quantitative trait loci (QTLs) and map-based cloning. In this study, we constructed a linkage map based on DNA samples from a commercial strain L808, including two parental monokaryons and 93 single spore isolates considered with segregating to 1:1:1:1 at four mating types (A(1)B(1), A(1)B(2), A(2)B(1) and A(2)B(2)). Using Simple Sequence Repeats (SSR), Sequence Related Amplified Polymorphism (SRAP), Target Region Amplified Polymorphism (TRAP) molecular markers, 182 molecular markers and two mating factors were located on 11 linkage groups (LGs). The total length of the map was 948.083 centimorgan (cM), with an average marker interval distance of 4.817 cM. Only two gaps spanning more than 20 cM was observed. The probability of 20 cM, 10 cM, 5 cM genetic distance cover one marker was 99.68%, 94.36%, 76.43% in our genetic linkage map, respectively. This is the first linkage map of Lentinula edodes using SSR markers, which provides essential information for quantitative trait analyses and improvement of genome assembly.
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spelling pubmed-69774512020-01-27 Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers Dong, Hui Shang, Xiaodong Zhao, Xiaoyan Yu, Hailong Jiang, Ning Zhang, Meiyan Tan, Qi Zhou, Changyan Zhang, Lujun Breed Sci Research Paper Genetic mapping is a basic tool for eukaryotic genomic research. It allows the localization of genes or quantitative trait loci (QTLs) and map-based cloning. In this study, we constructed a linkage map based on DNA samples from a commercial strain L808, including two parental monokaryons and 93 single spore isolates considered with segregating to 1:1:1:1 at four mating types (A(1)B(1), A(1)B(2), A(2)B(1) and A(2)B(2)). Using Simple Sequence Repeats (SSR), Sequence Related Amplified Polymorphism (SRAP), Target Region Amplified Polymorphism (TRAP) molecular markers, 182 molecular markers and two mating factors were located on 11 linkage groups (LGs). The total length of the map was 948.083 centimorgan (cM), with an average marker interval distance of 4.817 cM. Only two gaps spanning more than 20 cM was observed. The probability of 20 cM, 10 cM, 5 cM genetic distance cover one marker was 99.68%, 94.36%, 76.43% in our genetic linkage map, respectively. This is the first linkage map of Lentinula edodes using SSR markers, which provides essential information for quantitative trait analyses and improvement of genome assembly. Japanese Society of Breeding 2019-12 2019-09-19 /pmc/articles/PMC6977451/ /pubmed/31988622 http://dx.doi.org/10.1270/jsbbs.18123 Text en Copyright © 2019 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Dong, Hui
Shang, Xiaodong
Zhao, Xiaoyan
Yu, Hailong
Jiang, Ning
Zhang, Meiyan
Tan, Qi
Zhou, Changyan
Zhang, Lujun
Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers
title Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers
title_full Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers
title_fullStr Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers
title_full_unstemmed Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers
title_short Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers
title_sort construction of a genetic linkage map of lentinula edodes based on ssr, srap and trap markers
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977451/
https://www.ncbi.nlm.nih.gov/pubmed/31988622
http://dx.doi.org/10.1270/jsbbs.18123
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