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PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells

[Image: see text] Introduction: Currently, drug-induced reactive oxygen species (ROS) mediating apoptosis pathway have extensively been investigated in designing effective strategies for colorectal cancer (CRC) chemotherapy. Bovine pancreatic ribonuclease A (RNase A) represents a new class of cytoto...

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Autores principales: Akbarzadeh Khiavi, Mostafa, Safary, Azam, Barar, Jaleh, Farzi-Khajeh, Hamed, Barzegari, Abolfazl, Mousavi, Rahimeh, Somi, Mohammad Hossein, Omidi, Yadollah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977588/
https://www.ncbi.nlm.nih.gov/pubmed/31988854
http://dx.doi.org/10.15171/bi.2020.04
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author Akbarzadeh Khiavi, Mostafa
Safary, Azam
Barar, Jaleh
Farzi-Khajeh, Hamed
Barzegari, Abolfazl
Mousavi, Rahimeh
Somi, Mohammad Hossein
Omidi, Yadollah
author_facet Akbarzadeh Khiavi, Mostafa
Safary, Azam
Barar, Jaleh
Farzi-Khajeh, Hamed
Barzegari, Abolfazl
Mousavi, Rahimeh
Somi, Mohammad Hossein
Omidi, Yadollah
author_sort Akbarzadeh Khiavi, Mostafa
collection PubMed
description [Image: see text] Introduction: Currently, drug-induced reactive oxygen species (ROS) mediating apoptosis pathway have extensively been investigated in designing effective strategies for colorectal cancer (CRC) chemotherapy. Bovine pancreatic ribonuclease A (RNase A) represents a new class of cytotoxic and non-mutagenic enzymes, and has gained more attention as a potential anticancer modality; however, the cytosolic ribonuclease inhibitors (RIs) restrict the clinical application of this enzyme. Nowadays, nanotechnology-based diagnostic and therapeutic systems have provided potential solutions for cancer treatments. Methods: In this study, the gold nanoparticles (AuNPs) were synthesized, stabilized by polyethylene glycol (PEG), functionalized, and covalently conjugated with RNase A. The physicochemical properties of engineered nanobiomedicine (AuNPs-PEG-RNase A) were characterized by scanning electron microscope (SEM), dynamic light scattering (DLS), and UV-vis spectrum. Then, its biological impacts including cell viability, apoptosis, and ROS production were evaluated in the SW-480 cells. Results: The engineered nanobiomedicine, AuNPs-PEG-RNase A, was found to effectively induce apoptosis in SW-480 cells and result in a significant reduction in cancer cell viability. Besides, the maximum production of ROS was obtained after the treatment of cells with an IC50 dose of AuNPs-PEG-RNase A. Conclusion: Based on the efficient ROS-responsiveness and the anticancer activity of RNase A of the engineered nanomedicine, this nanoscaled biologics may be considered as a potential candidate for the treatment of CRC.
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spelling pubmed-69775882020-01-27 PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells Akbarzadeh Khiavi, Mostafa Safary, Azam Barar, Jaleh Farzi-Khajeh, Hamed Barzegari, Abolfazl Mousavi, Rahimeh Somi, Mohammad Hossein Omidi, Yadollah Bioimpacts Original Research [Image: see text] Introduction: Currently, drug-induced reactive oxygen species (ROS) mediating apoptosis pathway have extensively been investigated in designing effective strategies for colorectal cancer (CRC) chemotherapy. Bovine pancreatic ribonuclease A (RNase A) represents a new class of cytotoxic and non-mutagenic enzymes, and has gained more attention as a potential anticancer modality; however, the cytosolic ribonuclease inhibitors (RIs) restrict the clinical application of this enzyme. Nowadays, nanotechnology-based diagnostic and therapeutic systems have provided potential solutions for cancer treatments. Methods: In this study, the gold nanoparticles (AuNPs) were synthesized, stabilized by polyethylene glycol (PEG), functionalized, and covalently conjugated with RNase A. The physicochemical properties of engineered nanobiomedicine (AuNPs-PEG-RNase A) were characterized by scanning electron microscope (SEM), dynamic light scattering (DLS), and UV-vis spectrum. Then, its biological impacts including cell viability, apoptosis, and ROS production were evaluated in the SW-480 cells. Results: The engineered nanobiomedicine, AuNPs-PEG-RNase A, was found to effectively induce apoptosis in SW-480 cells and result in a significant reduction in cancer cell viability. Besides, the maximum production of ROS was obtained after the treatment of cells with an IC50 dose of AuNPs-PEG-RNase A. Conclusion: Based on the efficient ROS-responsiveness and the anticancer activity of RNase A of the engineered nanomedicine, this nanoscaled biologics may be considered as a potential candidate for the treatment of CRC. Tabriz University of Medical Sciences 2020 2019-07-25 /pmc/articles/PMC6977588/ /pubmed/31988854 http://dx.doi.org/10.15171/bi.2020.04 Text en © 2020 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Research
Akbarzadeh Khiavi, Mostafa
Safary, Azam
Barar, Jaleh
Farzi-Khajeh, Hamed
Barzegari, Abolfazl
Mousavi, Rahimeh
Somi, Mohammad Hossein
Omidi, Yadollah
PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells
title PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells
title_full PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells
title_fullStr PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells
title_full_unstemmed PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells
title_short PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells
title_sort pegylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977588/
https://www.ncbi.nlm.nih.gov/pubmed/31988854
http://dx.doi.org/10.15171/bi.2020.04
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