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PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells
[Image: see text] Introduction: Currently, drug-induced reactive oxygen species (ROS) mediating apoptosis pathway have extensively been investigated in designing effective strategies for colorectal cancer (CRC) chemotherapy. Bovine pancreatic ribonuclease A (RNase A) represents a new class of cytoto...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tabriz University of Medical Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977588/ https://www.ncbi.nlm.nih.gov/pubmed/31988854 http://dx.doi.org/10.15171/bi.2020.04 |
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author | Akbarzadeh Khiavi, Mostafa Safary, Azam Barar, Jaleh Farzi-Khajeh, Hamed Barzegari, Abolfazl Mousavi, Rahimeh Somi, Mohammad Hossein Omidi, Yadollah |
author_facet | Akbarzadeh Khiavi, Mostafa Safary, Azam Barar, Jaleh Farzi-Khajeh, Hamed Barzegari, Abolfazl Mousavi, Rahimeh Somi, Mohammad Hossein Omidi, Yadollah |
author_sort | Akbarzadeh Khiavi, Mostafa |
collection | PubMed |
description | [Image: see text] Introduction: Currently, drug-induced reactive oxygen species (ROS) mediating apoptosis pathway have extensively been investigated in designing effective strategies for colorectal cancer (CRC) chemotherapy. Bovine pancreatic ribonuclease A (RNase A) represents a new class of cytotoxic and non-mutagenic enzymes, and has gained more attention as a potential anticancer modality; however, the cytosolic ribonuclease inhibitors (RIs) restrict the clinical application of this enzyme. Nowadays, nanotechnology-based diagnostic and therapeutic systems have provided potential solutions for cancer treatments. Methods: In this study, the gold nanoparticles (AuNPs) were synthesized, stabilized by polyethylene glycol (PEG), functionalized, and covalently conjugated with RNase A. The physicochemical properties of engineered nanobiomedicine (AuNPs-PEG-RNase A) were characterized by scanning electron microscope (SEM), dynamic light scattering (DLS), and UV-vis spectrum. Then, its biological impacts including cell viability, apoptosis, and ROS production were evaluated in the SW-480 cells. Results: The engineered nanobiomedicine, AuNPs-PEG-RNase A, was found to effectively induce apoptosis in SW-480 cells and result in a significant reduction in cancer cell viability. Besides, the maximum production of ROS was obtained after the treatment of cells with an IC50 dose of AuNPs-PEG-RNase A. Conclusion: Based on the efficient ROS-responsiveness and the anticancer activity of RNase A of the engineered nanomedicine, this nanoscaled biologics may be considered as a potential candidate for the treatment of CRC. |
format | Online Article Text |
id | pubmed-6977588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Tabriz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-69775882020-01-27 PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells Akbarzadeh Khiavi, Mostafa Safary, Azam Barar, Jaleh Farzi-Khajeh, Hamed Barzegari, Abolfazl Mousavi, Rahimeh Somi, Mohammad Hossein Omidi, Yadollah Bioimpacts Original Research [Image: see text] Introduction: Currently, drug-induced reactive oxygen species (ROS) mediating apoptosis pathway have extensively been investigated in designing effective strategies for colorectal cancer (CRC) chemotherapy. Bovine pancreatic ribonuclease A (RNase A) represents a new class of cytotoxic and non-mutagenic enzymes, and has gained more attention as a potential anticancer modality; however, the cytosolic ribonuclease inhibitors (RIs) restrict the clinical application of this enzyme. Nowadays, nanotechnology-based diagnostic and therapeutic systems have provided potential solutions for cancer treatments. Methods: In this study, the gold nanoparticles (AuNPs) were synthesized, stabilized by polyethylene glycol (PEG), functionalized, and covalently conjugated with RNase A. The physicochemical properties of engineered nanobiomedicine (AuNPs-PEG-RNase A) were characterized by scanning electron microscope (SEM), dynamic light scattering (DLS), and UV-vis spectrum. Then, its biological impacts including cell viability, apoptosis, and ROS production were evaluated in the SW-480 cells. Results: The engineered nanobiomedicine, AuNPs-PEG-RNase A, was found to effectively induce apoptosis in SW-480 cells and result in a significant reduction in cancer cell viability. Besides, the maximum production of ROS was obtained after the treatment of cells with an IC50 dose of AuNPs-PEG-RNase A. Conclusion: Based on the efficient ROS-responsiveness and the anticancer activity of RNase A of the engineered nanomedicine, this nanoscaled biologics may be considered as a potential candidate for the treatment of CRC. Tabriz University of Medical Sciences 2020 2019-07-25 /pmc/articles/PMC6977588/ /pubmed/31988854 http://dx.doi.org/10.15171/bi.2020.04 Text en © 2020 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Research Akbarzadeh Khiavi, Mostafa Safary, Azam Barar, Jaleh Farzi-Khajeh, Hamed Barzegari, Abolfazl Mousavi, Rahimeh Somi, Mohammad Hossein Omidi, Yadollah PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells |
title | PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells |
title_full | PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells |
title_fullStr | PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells |
title_full_unstemmed | PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells |
title_short | PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells |
title_sort | pegylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977588/ https://www.ncbi.nlm.nih.gov/pubmed/31988854 http://dx.doi.org/10.15171/bi.2020.04 |
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