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PRDX5 as a novel binding partner in Nrf2-mediated NSCLC progression under oxidative stress

Non-small-cell lung cancer (NSCLC) is one of the most common malignant tumors in the world. Reactive oxidative species (ROS) and nuclear factor-related factor 2 (Nrf2) -antioxidant response element (ARE) signal pathway are known to play important roles in the development of NSCLC. In this study, we...

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Autores principales: Chen, Xinming, Cao, Xiang, Xiao, Weizhang, Li, Ben, Xue, Qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977694/
https://www.ncbi.nlm.nih.gov/pubmed/31899687
http://dx.doi.org/10.18632/aging.102605
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author Chen, Xinming
Cao, Xiang
Xiao, Weizhang
Li, Ben
Xue, Qun
author_facet Chen, Xinming
Cao, Xiang
Xiao, Weizhang
Li, Ben
Xue, Qun
author_sort Chen, Xinming
collection PubMed
description Non-small-cell lung cancer (NSCLC) is one of the most common malignant tumors in the world. Reactive oxidative species (ROS) and nuclear factor-related factor 2 (Nrf2) -antioxidant response element (ARE) signal pathway are known to play important roles in the development of NSCLC. In this study, we identified Peroxiredoxin 5 (PRDX5) as a novel binding partner for Nrf2. PRDX5 was significantly increased in human NSCLC specimens and cell lines. Nrf2 interacted with PRDX5 in H(2)O(2)-stimulated NCSLC cells, and the interaction promoted the expression of NAD(P)H: quinone oxidoreductase 1 (NQO1) protein in NSCLC cells. Further, high expression of Nrf2 and PRDX5 were associated with worsened prognosis in patients with NSCLC significantly. Moreover, animal studies showed that the growth of tumors treated with Nrf2 and PRDX5 shRNA was significantly lower than that of the other groups. All these data indicated that overexpressed PRDX5 in NSCLC promoted binding with Nrf2 and enhanced NQO1 expression and NSCLC development. Overall, our studies demonstrated that PRDX5 can be a novel binding partner of Nrf2 in promoting NCSLC development under oxidative stress and provide potential opportunity for improving NSCLC therapy.
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spelling pubmed-69776942020-01-31 PRDX5 as a novel binding partner in Nrf2-mediated NSCLC progression under oxidative stress Chen, Xinming Cao, Xiang Xiao, Weizhang Li, Ben Xue, Qun Aging (Albany NY) Research Paper Non-small-cell lung cancer (NSCLC) is one of the most common malignant tumors in the world. Reactive oxidative species (ROS) and nuclear factor-related factor 2 (Nrf2) -antioxidant response element (ARE) signal pathway are known to play important roles in the development of NSCLC. In this study, we identified Peroxiredoxin 5 (PRDX5) as a novel binding partner for Nrf2. PRDX5 was significantly increased in human NSCLC specimens and cell lines. Nrf2 interacted with PRDX5 in H(2)O(2)-stimulated NCSLC cells, and the interaction promoted the expression of NAD(P)H: quinone oxidoreductase 1 (NQO1) protein in NSCLC cells. Further, high expression of Nrf2 and PRDX5 were associated with worsened prognosis in patients with NSCLC significantly. Moreover, animal studies showed that the growth of tumors treated with Nrf2 and PRDX5 shRNA was significantly lower than that of the other groups. All these data indicated that overexpressed PRDX5 in NSCLC promoted binding with Nrf2 and enhanced NQO1 expression and NSCLC development. Overall, our studies demonstrated that PRDX5 can be a novel binding partner of Nrf2 in promoting NCSLC development under oxidative stress and provide potential opportunity for improving NSCLC therapy. Impact Journals 2020-01-03 /pmc/articles/PMC6977694/ /pubmed/31899687 http://dx.doi.org/10.18632/aging.102605 Text en Copyright © 2020 Chen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Chen, Xinming
Cao, Xiang
Xiao, Weizhang
Li, Ben
Xue, Qun
PRDX5 as a novel binding partner in Nrf2-mediated NSCLC progression under oxidative stress
title PRDX5 as a novel binding partner in Nrf2-mediated NSCLC progression under oxidative stress
title_full PRDX5 as a novel binding partner in Nrf2-mediated NSCLC progression under oxidative stress
title_fullStr PRDX5 as a novel binding partner in Nrf2-mediated NSCLC progression under oxidative stress
title_full_unstemmed PRDX5 as a novel binding partner in Nrf2-mediated NSCLC progression under oxidative stress
title_short PRDX5 as a novel binding partner in Nrf2-mediated NSCLC progression under oxidative stress
title_sort prdx5 as a novel binding partner in nrf2-mediated nsclc progression under oxidative stress
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977694/
https://www.ncbi.nlm.nih.gov/pubmed/31899687
http://dx.doi.org/10.18632/aging.102605
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