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Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a ‘zero-step’ RT-qPCR protocol

We describe an ultra-rapid and sensitive method to quantify gene expression levels in cultured cells. The procedure is based on reverse-transcription quantitative PCR (RT-qPCR) directly from cells, without RNA extraction and without an isothermal reverse-transcription step. Human neurons (Lund human...

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Detalles Bibliográficos
Autores principales:	Chovancova, Petra, Merk, Verena, Marx, Andreas, Leist, Marcel, Kranaster, Ramon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Manuscript
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977950/ https://www.ncbi.nlm.nih.gov/pubmed/32002469 http://dx.doi.org/10.1093/biomethods/bpx008

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