Surface Charge Mapping on Virions and Virus-Like Particles of Helical Plant Viruses

Currently, the assembly of helical plant viruses is poorly understood. The viral assembly and infection may be affected by the charge distribution on the virion surface. However, only the total virion charge (isoelectric point) has been determined for most plant viruses. Here, we report on the first...

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Detalles Bibliográficos
Autores principales: Arkhipenko, M. V., Nikitin, N. A., Baranov, O. A., Evtushenko, E. A., Atabekov, J. G., Karpova, O. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977955/
https://www.ncbi.nlm.nih.gov/pubmed/31993237
http://dx.doi.org/10.32607/20758251-2019-11-4-73-78
Descripción
Sumario:Currently, the assembly of helical plant viruses is poorly understood. The viral assembly and infection may be affected by the charge distribution on the virion surface. However, only the total virion charge (isoelectric point) has been determined for most plant viruses. Here, we report on the first application of positively charged magnetic nanoparticles for mapping the surface charge distribution of helical plant viruses. The charge was demonstrated to be unevenly distributed on the surface of viruses belonging to different taxonomic groups, with the negative charge being predominantly located at one end of the virions. This charge distribution is mainly controlled by viral RNA.

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