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Effect of exosomes from adipose‐derived stem cells on the apoptosis of Schwann cells in peripheral nerve injury

AIMS: Recovery after peripheral nerve injury (PNI) is often difficult, and there is no optimal treatment. Schwann cells (SCs) are important for peripheral nerve regeneration, so SC‐targeting treatments have gained importance. Adipose‐derived stem cells (ADSCs) and their exosomes can promote peripher...

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Autores principales: Liu, Cai‐Yue, Yin, Gang, Sun, Yi‐Dan, Lin, Yao‐Fa, Xie, Zheng, English, Arthur W., Li, Qing‐Feng, Lin, Hao‐Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978230/
https://www.ncbi.nlm.nih.gov/pubmed/31278850
http://dx.doi.org/10.1111/cns.13187
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author Liu, Cai‐Yue
Yin, Gang
Sun, Yi‐Dan
Lin, Yao‐Fa
Xie, Zheng
English, Arthur W.
Li, Qing‐Feng
Lin, Hao‐Dong
author_facet Liu, Cai‐Yue
Yin, Gang
Sun, Yi‐Dan
Lin, Yao‐Fa
Xie, Zheng
English, Arthur W.
Li, Qing‐Feng
Lin, Hao‐Dong
author_sort Liu, Cai‐Yue
collection PubMed
description AIMS: Recovery after peripheral nerve injury (PNI) is often difficult, and there is no optimal treatment. Schwann cells (SCs) are important for peripheral nerve regeneration, so SC‐targeting treatments have gained importance. Adipose‐derived stem cells (ADSCs) and their exosomes can promote peripheral nerve repair, but their interactions with SCs are unclear. METHODS: Purified SCs from sciatic nerve injury sites were harvested, and apoptosis and proliferation of SCs at post‐PNI 24 hours were analyzed. The effects of coculture with ADSCs and different concentrations of ADSC‐derived exosomes (ADSC‐Exo) were studied through in vitro experiments by flow cytometry, CCK8 assay, immunofluorescence staining, and histological analysis. The expression of the apoptosis‐related genes Bcl‐2 and Bax was also analyzed by qRT‐PCR. RESULTS: ADSC‐Exo reduced the apoptosis of SCs after PNI by upregulating the anti‐apoptotic Bcl‐2 mRNA expression and downregulating the pro‐apoptotic Bax mRNA expression. Further, it also improved the proliferation rate of SCs. This effect was confirmed by the morphological and histological findings in PNI model rats. CONCLUSION: Our results present a novel exosome‐mediated mechanism for ADSC‐SC cross talk that reduces the apoptosis and promotes the proliferation of SCs and may have therapeutic potential in the future.
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spelling pubmed-69782302020-01-28 Effect of exosomes from adipose‐derived stem cells on the apoptosis of Schwann cells in peripheral nerve injury Liu, Cai‐Yue Yin, Gang Sun, Yi‐Dan Lin, Yao‐Fa Xie, Zheng English, Arthur W. Li, Qing‐Feng Lin, Hao‐Dong CNS Neurosci Ther Original Articles AIMS: Recovery after peripheral nerve injury (PNI) is often difficult, and there is no optimal treatment. Schwann cells (SCs) are important for peripheral nerve regeneration, so SC‐targeting treatments have gained importance. Adipose‐derived stem cells (ADSCs) and their exosomes can promote peripheral nerve repair, but their interactions with SCs are unclear. METHODS: Purified SCs from sciatic nerve injury sites were harvested, and apoptosis and proliferation of SCs at post‐PNI 24 hours were analyzed. The effects of coculture with ADSCs and different concentrations of ADSC‐derived exosomes (ADSC‐Exo) were studied through in vitro experiments by flow cytometry, CCK8 assay, immunofluorescence staining, and histological analysis. The expression of the apoptosis‐related genes Bcl‐2 and Bax was also analyzed by qRT‐PCR. RESULTS: ADSC‐Exo reduced the apoptosis of SCs after PNI by upregulating the anti‐apoptotic Bcl‐2 mRNA expression and downregulating the pro‐apoptotic Bax mRNA expression. Further, it also improved the proliferation rate of SCs. This effect was confirmed by the morphological and histological findings in PNI model rats. CONCLUSION: Our results present a novel exosome‐mediated mechanism for ADSC‐SC cross talk that reduces the apoptosis and promotes the proliferation of SCs and may have therapeutic potential in the future. John Wiley and Sons Inc. 2019-07-06 /pmc/articles/PMC6978230/ /pubmed/31278850 http://dx.doi.org/10.1111/cns.13187 Text en © 2019 The Authors. CNS Neuroscience & Therapeutics Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Liu, Cai‐Yue
Yin, Gang
Sun, Yi‐Dan
Lin, Yao‐Fa
Xie, Zheng
English, Arthur W.
Li, Qing‐Feng
Lin, Hao‐Dong
Effect of exosomes from adipose‐derived stem cells on the apoptosis of Schwann cells in peripheral nerve injury
title Effect of exosomes from adipose‐derived stem cells on the apoptosis of Schwann cells in peripheral nerve injury
title_full Effect of exosomes from adipose‐derived stem cells on the apoptosis of Schwann cells in peripheral nerve injury
title_fullStr Effect of exosomes from adipose‐derived stem cells on the apoptosis of Schwann cells in peripheral nerve injury
title_full_unstemmed Effect of exosomes from adipose‐derived stem cells on the apoptosis of Schwann cells in peripheral nerve injury
title_short Effect of exosomes from adipose‐derived stem cells on the apoptosis of Schwann cells in peripheral nerve injury
title_sort effect of exosomes from adipose‐derived stem cells on the apoptosis of schwann cells in peripheral nerve injury
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978230/
https://www.ncbi.nlm.nih.gov/pubmed/31278850
http://dx.doi.org/10.1111/cns.13187
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