Cargando…
Alternative transcription cycle for bacterial RNA polymerase
RNA polymerases (RNAPs) transcribe genes through a cycle of recruitment to promoter DNA, initiation, elongation, and termination. After termination, RNAP is thought to initiate the next round of transcription by detaching from DNA and rebinding a new promoter. Here we use single-molecule fluorescenc...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978322/ https://www.ncbi.nlm.nih.gov/pubmed/31974358 http://dx.doi.org/10.1038/s41467-019-14208-9 |
_version_ | 1783490673202167808 |
---|---|
author | Harden, Timothy T. Herlambang, Karina S. Chamberlain, Mathew Lalanne, Jean-Benoît Wells, Christopher D. Li, Gene-Wei Landick, Robert Hochschild, Ann Kondev, Jane Gelles, Jeff |
author_facet | Harden, Timothy T. Herlambang, Karina S. Chamberlain, Mathew Lalanne, Jean-Benoît Wells, Christopher D. Li, Gene-Wei Landick, Robert Hochschild, Ann Kondev, Jane Gelles, Jeff |
author_sort | Harden, Timothy T. |
collection | PubMed |
description | RNA polymerases (RNAPs) transcribe genes through a cycle of recruitment to promoter DNA, initiation, elongation, and termination. After termination, RNAP is thought to initiate the next round of transcription by detaching from DNA and rebinding a new promoter. Here we use single-molecule fluorescence microscopy to observe individual RNAP molecules after transcript release at a terminator. Following termination, RNAP almost always remains bound to DNA and sometimes exhibits one-dimensional sliding over thousands of basepairs. Unexpectedly, the DNA-bound RNAP often restarts transcription, usually in reverse direction, thus producing an antisense transcript. Furthermore, we report evidence of this secondary initiation in live cells, using genome-wide RNA sequencing. These findings reveal an alternative transcription cycle that allows RNAP to reinitiate without dissociating from DNA, which is likely to have important implications for gene regulation. |
format | Online Article Text |
id | pubmed-6978322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69783222020-01-27 Alternative transcription cycle for bacterial RNA polymerase Harden, Timothy T. Herlambang, Karina S. Chamberlain, Mathew Lalanne, Jean-Benoît Wells, Christopher D. Li, Gene-Wei Landick, Robert Hochschild, Ann Kondev, Jane Gelles, Jeff Nat Commun Article RNA polymerases (RNAPs) transcribe genes through a cycle of recruitment to promoter DNA, initiation, elongation, and termination. After termination, RNAP is thought to initiate the next round of transcription by detaching from DNA and rebinding a new promoter. Here we use single-molecule fluorescence microscopy to observe individual RNAP molecules after transcript release at a terminator. Following termination, RNAP almost always remains bound to DNA and sometimes exhibits one-dimensional sliding over thousands of basepairs. Unexpectedly, the DNA-bound RNAP often restarts transcription, usually in reverse direction, thus producing an antisense transcript. Furthermore, we report evidence of this secondary initiation in live cells, using genome-wide RNA sequencing. These findings reveal an alternative transcription cycle that allows RNAP to reinitiate without dissociating from DNA, which is likely to have important implications for gene regulation. Nature Publishing Group UK 2020-01-23 /pmc/articles/PMC6978322/ /pubmed/31974358 http://dx.doi.org/10.1038/s41467-019-14208-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Harden, Timothy T. Herlambang, Karina S. Chamberlain, Mathew Lalanne, Jean-Benoît Wells, Christopher D. Li, Gene-Wei Landick, Robert Hochschild, Ann Kondev, Jane Gelles, Jeff Alternative transcription cycle for bacterial RNA polymerase |
title | Alternative transcription cycle for bacterial RNA polymerase |
title_full | Alternative transcription cycle for bacterial RNA polymerase |
title_fullStr | Alternative transcription cycle for bacterial RNA polymerase |
title_full_unstemmed | Alternative transcription cycle for bacterial RNA polymerase |
title_short | Alternative transcription cycle for bacterial RNA polymerase |
title_sort | alternative transcription cycle for bacterial rna polymerase |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978322/ https://www.ncbi.nlm.nih.gov/pubmed/31974358 http://dx.doi.org/10.1038/s41467-019-14208-9 |
work_keys_str_mv | AT hardentimothyt alternativetranscriptioncycleforbacterialrnapolymerase AT herlambangkarinas alternativetranscriptioncycleforbacterialrnapolymerase AT chamberlainmathew alternativetranscriptioncycleforbacterialrnapolymerase AT lalannejeanbenoit alternativetranscriptioncycleforbacterialrnapolymerase AT wellschristopherd alternativetranscriptioncycleforbacterialrnapolymerase AT ligenewei alternativetranscriptioncycleforbacterialrnapolymerase AT landickrobert alternativetranscriptioncycleforbacterialrnapolymerase AT hochschildann alternativetranscriptioncycleforbacterialrnapolymerase AT kondevjane alternativetranscriptioncycleforbacterialrnapolymerase AT gellesjeff alternativetranscriptioncycleforbacterialrnapolymerase |