Cargando…

Alternative transcription cycle for bacterial RNA polymerase

RNA polymerases (RNAPs) transcribe genes through a cycle of recruitment to promoter DNA, initiation, elongation, and termination. After termination, RNAP is thought to initiate the next round of transcription by detaching from DNA and rebinding a new promoter. Here we use single-molecule fluorescenc...

Descripción completa

Detalles Bibliográficos
Autores principales: Harden, Timothy T., Herlambang, Karina S., Chamberlain, Mathew, Lalanne, Jean-Benoît, Wells, Christopher D., Li, Gene-Wei, Landick, Robert, Hochschild, Ann, Kondev, Jane, Gelles, Jeff
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978322/
https://www.ncbi.nlm.nih.gov/pubmed/31974358
http://dx.doi.org/10.1038/s41467-019-14208-9
_version_ 1783490673202167808
author Harden, Timothy T.
Herlambang, Karina S.
Chamberlain, Mathew
Lalanne, Jean-Benoît
Wells, Christopher D.
Li, Gene-Wei
Landick, Robert
Hochschild, Ann
Kondev, Jane
Gelles, Jeff
author_facet Harden, Timothy T.
Herlambang, Karina S.
Chamberlain, Mathew
Lalanne, Jean-Benoît
Wells, Christopher D.
Li, Gene-Wei
Landick, Robert
Hochschild, Ann
Kondev, Jane
Gelles, Jeff
author_sort Harden, Timothy T.
collection PubMed
description RNA polymerases (RNAPs) transcribe genes through a cycle of recruitment to promoter DNA, initiation, elongation, and termination. After termination, RNAP is thought to initiate the next round of transcription by detaching from DNA and rebinding a new promoter. Here we use single-molecule fluorescence microscopy to observe individual RNAP molecules after transcript release at a terminator. Following termination, RNAP almost always remains bound to DNA and sometimes exhibits one-dimensional sliding over thousands of basepairs. Unexpectedly, the DNA-bound RNAP often restarts transcription, usually in reverse direction, thus producing an antisense transcript. Furthermore, we report evidence of this secondary initiation in live cells, using genome-wide RNA sequencing. These findings reveal an alternative transcription cycle that allows RNAP to reinitiate without dissociating from DNA, which is likely to have important implications for gene regulation.
format Online
Article
Text
id pubmed-6978322
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-69783222020-01-27 Alternative transcription cycle for bacterial RNA polymerase Harden, Timothy T. Herlambang, Karina S. Chamberlain, Mathew Lalanne, Jean-Benoît Wells, Christopher D. Li, Gene-Wei Landick, Robert Hochschild, Ann Kondev, Jane Gelles, Jeff Nat Commun Article RNA polymerases (RNAPs) transcribe genes through a cycle of recruitment to promoter DNA, initiation, elongation, and termination. After termination, RNAP is thought to initiate the next round of transcription by detaching from DNA and rebinding a new promoter. Here we use single-molecule fluorescence microscopy to observe individual RNAP molecules after transcript release at a terminator. Following termination, RNAP almost always remains bound to DNA and sometimes exhibits one-dimensional sliding over thousands of basepairs. Unexpectedly, the DNA-bound RNAP often restarts transcription, usually in reverse direction, thus producing an antisense transcript. Furthermore, we report evidence of this secondary initiation in live cells, using genome-wide RNA sequencing. These findings reveal an alternative transcription cycle that allows RNAP to reinitiate without dissociating from DNA, which is likely to have important implications for gene regulation. Nature Publishing Group UK 2020-01-23 /pmc/articles/PMC6978322/ /pubmed/31974358 http://dx.doi.org/10.1038/s41467-019-14208-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Harden, Timothy T.
Herlambang, Karina S.
Chamberlain, Mathew
Lalanne, Jean-Benoît
Wells, Christopher D.
Li, Gene-Wei
Landick, Robert
Hochschild, Ann
Kondev, Jane
Gelles, Jeff
Alternative transcription cycle for bacterial RNA polymerase
title Alternative transcription cycle for bacterial RNA polymerase
title_full Alternative transcription cycle for bacterial RNA polymerase
title_fullStr Alternative transcription cycle for bacterial RNA polymerase
title_full_unstemmed Alternative transcription cycle for bacterial RNA polymerase
title_short Alternative transcription cycle for bacterial RNA polymerase
title_sort alternative transcription cycle for bacterial rna polymerase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978322/
https://www.ncbi.nlm.nih.gov/pubmed/31974358
http://dx.doi.org/10.1038/s41467-019-14208-9
work_keys_str_mv AT hardentimothyt alternativetranscriptioncycleforbacterialrnapolymerase
AT herlambangkarinas alternativetranscriptioncycleforbacterialrnapolymerase
AT chamberlainmathew alternativetranscriptioncycleforbacterialrnapolymerase
AT lalannejeanbenoit alternativetranscriptioncycleforbacterialrnapolymerase
AT wellschristopherd alternativetranscriptioncycleforbacterialrnapolymerase
AT ligenewei alternativetranscriptioncycleforbacterialrnapolymerase
AT landickrobert alternativetranscriptioncycleforbacterialrnapolymerase
AT hochschildann alternativetranscriptioncycleforbacterialrnapolymerase
AT kondevjane alternativetranscriptioncycleforbacterialrnapolymerase
AT gellesjeff alternativetranscriptioncycleforbacterialrnapolymerase