A biphasic growth model for cell pole elongation in mycobacteria

Mycobacteria grow by inserting new cell wall material in discrete zones at the cell poles. This pattern implies that polar growth zones must be assembled de novo at each division, but the mechanisms that control the initiation of new pole growth are unknown. Here, we combine time-lapse optical and a...

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Autores principales: Hannebelle, Mélanie T. M., Ven, Joëlle X. Y., Toniolo, Chiara, Eskandarian, Haig A., Vuaridel-Thurre, Gaëlle, McKinney, John D., Fantner, Georg E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978421/
https://www.ncbi.nlm.nih.gov/pubmed/31974342
http://dx.doi.org/10.1038/s41467-019-14088-z
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author Hannebelle, Mélanie T. M.
Ven, Joëlle X. Y.
Toniolo, Chiara
Eskandarian, Haig A.
Vuaridel-Thurre, Gaëlle
McKinney, John D.
Fantner, Georg E.
author_facet Hannebelle, Mélanie T. M.
Ven, Joëlle X. Y.
Toniolo, Chiara
Eskandarian, Haig A.
Vuaridel-Thurre, Gaëlle
McKinney, John D.
Fantner, Georg E.
author_sort Hannebelle, Mélanie T. M.
collection PubMed
description Mycobacteria grow by inserting new cell wall material in discrete zones at the cell poles. This pattern implies that polar growth zones must be assembled de novo at each division, but the mechanisms that control the initiation of new pole growth are unknown. Here, we combine time-lapse optical and atomic force microscopy to measure single-cell pole growth in mycobacteria with nanometer-scale precision. We show that single-cell growth is biphasic due to a lag phase of variable duration before the new pole transitions from slow to fast growth. This transition and cell division are independent events. The difference between the lag and interdivision times determines the degree of single-cell growth asymmetry, which is high in fast-growing species and low in slow-growing species. We propose a biphasic growth model that is distinct from previous unipolar and bipolar models and resembles “new end take off” (NETO) dynamics of polar growth in fission yeast.
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spelling pubmed-69784212020-01-27 A biphasic growth model for cell pole elongation in mycobacteria Hannebelle, Mélanie T. M. Ven, Joëlle X. Y. Toniolo, Chiara Eskandarian, Haig A. Vuaridel-Thurre, Gaëlle McKinney, John D. Fantner, Georg E. Nat Commun Article Mycobacteria grow by inserting new cell wall material in discrete zones at the cell poles. This pattern implies that polar growth zones must be assembled de novo at each division, but the mechanisms that control the initiation of new pole growth are unknown. Here, we combine time-lapse optical and atomic force microscopy to measure single-cell pole growth in mycobacteria with nanometer-scale precision. We show that single-cell growth is biphasic due to a lag phase of variable duration before the new pole transitions from slow to fast growth. This transition and cell division are independent events. The difference between the lag and interdivision times determines the degree of single-cell growth asymmetry, which is high in fast-growing species and low in slow-growing species. We propose a biphasic growth model that is distinct from previous unipolar and bipolar models and resembles “new end take off” (NETO) dynamics of polar growth in fission yeast. Nature Publishing Group UK 2020-01-23 /pmc/articles/PMC6978421/ /pubmed/31974342 http://dx.doi.org/10.1038/s41467-019-14088-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hannebelle, Mélanie T. M.
Ven, Joëlle X. Y.
Toniolo, Chiara
Eskandarian, Haig A.
Vuaridel-Thurre, Gaëlle
McKinney, John D.
Fantner, Georg E.
A biphasic growth model for cell pole elongation in mycobacteria
title A biphasic growth model for cell pole elongation in mycobacteria
title_full A biphasic growth model for cell pole elongation in mycobacteria
title_fullStr A biphasic growth model for cell pole elongation in mycobacteria
title_full_unstemmed A biphasic growth model for cell pole elongation in mycobacteria
title_short A biphasic growth model for cell pole elongation in mycobacteria
title_sort biphasic growth model for cell pole elongation in mycobacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978421/
https://www.ncbi.nlm.nih.gov/pubmed/31974342
http://dx.doi.org/10.1038/s41467-019-14088-z
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