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Enhancing biosensing sensitivity of metal nanostructures through site-selective binding
The localised surface plasmon resonance (LSPR) at the surface of metal nanostructures can induce a highly intense electromagnetic (EM) field, which is confined to the edges with big curvature or at narrow gaps between nanostructures. Therefore, the localisation of target molecules at these sites is...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978459/ https://www.ncbi.nlm.nih.gov/pubmed/31974422 http://dx.doi.org/10.1038/s41598-020-57791-4 |
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author | Jo, Na rae Shin, Yong-Beom |
author_facet | Jo, Na rae Shin, Yong-Beom |
author_sort | Jo, Na rae |
collection | PubMed |
description | The localised surface plasmon resonance (LSPR) at the surface of metal nanostructures can induce a highly intense electromagnetic (EM) field, which is confined to the edges with big curvature or at narrow gaps between nanostructures. Therefore, the localisation of target molecules at these sites is crucial to achieve high sensitivity in LSPR-based biosensors. To this end, we fabricated a 40 nm high gold nano-truncated cone (GNTC) array using thermal nanoimprint lithography. As the EM field is most intense at the side surface and relatively weak at the top surface of GNTC, we improved the detection sensitivity by blocking the top surface with oxides to limit adsorption of antibodies and antigens to the top surface. We observed the difference in sensitivity by detecting α-fetoprotein (AFP) on the oxide-capped and uncapped GNTC arrays through sandwich immunoassay and enzymatic precipitation. The capped GNTC array exhibited higher detection sensitivity than the uncapped one. Particularly, six-fold enhancement of sensitivity was achieved in the serum sample. We used atomic force microscopy and electron microscopy to validate that the deposition of the oxides on the top surface of GNTC effectively blocked the adsorption of the biomolecules and the target molecules were preferentially adsorbed on the side surfaces. |
format | Online Article Text |
id | pubmed-6978459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69784592020-01-30 Enhancing biosensing sensitivity of metal nanostructures through site-selective binding Jo, Na rae Shin, Yong-Beom Sci Rep Article The localised surface plasmon resonance (LSPR) at the surface of metal nanostructures can induce a highly intense electromagnetic (EM) field, which is confined to the edges with big curvature or at narrow gaps between nanostructures. Therefore, the localisation of target molecules at these sites is crucial to achieve high sensitivity in LSPR-based biosensors. To this end, we fabricated a 40 nm high gold nano-truncated cone (GNTC) array using thermal nanoimprint lithography. As the EM field is most intense at the side surface and relatively weak at the top surface of GNTC, we improved the detection sensitivity by blocking the top surface with oxides to limit adsorption of antibodies and antigens to the top surface. We observed the difference in sensitivity by detecting α-fetoprotein (AFP) on the oxide-capped and uncapped GNTC arrays through sandwich immunoassay and enzymatic precipitation. The capped GNTC array exhibited higher detection sensitivity than the uncapped one. Particularly, six-fold enhancement of sensitivity was achieved in the serum sample. We used atomic force microscopy and electron microscopy to validate that the deposition of the oxides on the top surface of GNTC effectively blocked the adsorption of the biomolecules and the target molecules were preferentially adsorbed on the side surfaces. Nature Publishing Group UK 2020-01-23 /pmc/articles/PMC6978459/ /pubmed/31974422 http://dx.doi.org/10.1038/s41598-020-57791-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jo, Na rae Shin, Yong-Beom Enhancing biosensing sensitivity of metal nanostructures through site-selective binding |
title | Enhancing biosensing sensitivity of metal nanostructures through site-selective binding |
title_full | Enhancing biosensing sensitivity of metal nanostructures through site-selective binding |
title_fullStr | Enhancing biosensing sensitivity of metal nanostructures through site-selective binding |
title_full_unstemmed | Enhancing biosensing sensitivity of metal nanostructures through site-selective binding |
title_short | Enhancing biosensing sensitivity of metal nanostructures through site-selective binding |
title_sort | enhancing biosensing sensitivity of metal nanostructures through site-selective binding |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978459/ https://www.ncbi.nlm.nih.gov/pubmed/31974422 http://dx.doi.org/10.1038/s41598-020-57791-4 |
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