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HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation
A new rapid, simple, sensitive and high performance thin layer chromatography (HPTLC) has been established for the simultaneous determination of ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice and herbal formulation. HPTLC method was carried out using ethyl acetate: acetone...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978614/ https://www.ncbi.nlm.nih.gov/pubmed/31997904 http://dx.doi.org/10.1016/j.jsps.2019.07.006 |
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author | Alam, Prawez Kamal, Y.T. Alqasoumi, Saleh Ibrahim Foudah, Ahmed Ibrahim Alqarni, Mohammed Hamed Yusufoglu, Hasan Soliman |
author_facet | Alam, Prawez Kamal, Y.T. Alqasoumi, Saleh Ibrahim Foudah, Ahmed Ibrahim Alqarni, Mohammed Hamed Yusufoglu, Hasan Soliman |
author_sort | Alam, Prawez |
collection | PubMed |
description | A new rapid, simple, sensitive and high performance thin layer chromatography (HPTLC) has been established for the simultaneous determination of ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice and herbal formulation. HPTLC method was carried out using ethyl acetate: acetone: water: formic acid, 10:6:2:2 (%, v/v/v/v)) on 20 × 10 cm glass coated silica gel 60 F(254) plates and scanned at 254 nm for ascorbic acid and gallic acid. Ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice were identified by comparing their single spot at R(f) = 0.54 ± 0.02 and R(f) = 0.83 ± 0.01 respectively. The value of regression equation (r(2) ≥ 0.9992) revealed a good linear relationship between peak area and amount of ascorbic acid and gallic acid in the range of 100–800 ng/band. The method was validated for precision, accuracy, robustness LOD and LOQ. The method proposed can be useful for routine determination of ascorbic acid and gallic acid in various crude as well as herbal formulations as a quality control tool. |
format | Online Article Text |
id | pubmed-6978614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-69786142020-01-29 HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation Alam, Prawez Kamal, Y.T. Alqasoumi, Saleh Ibrahim Foudah, Ahmed Ibrahim Alqarni, Mohammed Hamed Yusufoglu, Hasan Soliman Saudi Pharm J Article A new rapid, simple, sensitive and high performance thin layer chromatography (HPTLC) has been established for the simultaneous determination of ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice and herbal formulation. HPTLC method was carried out using ethyl acetate: acetone: water: formic acid, 10:6:2:2 (%, v/v/v/v)) on 20 × 10 cm glass coated silica gel 60 F(254) plates and scanned at 254 nm for ascorbic acid and gallic acid. Ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice were identified by comparing their single spot at R(f) = 0.54 ± 0.02 and R(f) = 0.83 ± 0.01 respectively. The value of regression equation (r(2) ≥ 0.9992) revealed a good linear relationship between peak area and amount of ascorbic acid and gallic acid in the range of 100–800 ng/band. The method was validated for precision, accuracy, robustness LOD and LOQ. The method proposed can be useful for routine determination of ascorbic acid and gallic acid in various crude as well as herbal formulations as a quality control tool. Elsevier 2019-11 2019-07-16 /pmc/articles/PMC6978614/ /pubmed/31997904 http://dx.doi.org/10.1016/j.jsps.2019.07.006 Text en © 2019 Production and hosting by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Alam, Prawez Kamal, Y.T. Alqasoumi, Saleh Ibrahim Foudah, Ahmed Ibrahim Alqarni, Mohammed Hamed Yusufoglu, Hasan Soliman HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation |
title | HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation |
title_full | HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation |
title_fullStr | HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation |
title_full_unstemmed | HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation |
title_short | HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation |
title_sort | hptlc method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978614/ https://www.ncbi.nlm.nih.gov/pubmed/31997904 http://dx.doi.org/10.1016/j.jsps.2019.07.006 |
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