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Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica

Fermentation of gases provides a promising opportunity for the production of biochemicals from renewable resources, which has resulted in a growing interest in acetogenic bacteria. Thermophilic organisms provide potential advantages for the fermentation of, e.g., syngas into for example volatile com...

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Autores principales: Redl, Stephanie, Poehlein, Anja, Esser, Carola, Bengelsdorf, Frank R., Jensen, Torbjørn Ø., Jendresen, Christian B., Tindall, Brian J., Daniel, Rolf, Dürre, Peter, Nielsen, Alex T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978639/
https://www.ncbi.nlm.nih.gov/pubmed/32010113
http://dx.doi.org/10.3389/fmicb.2019.03070
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author Redl, Stephanie
Poehlein, Anja
Esser, Carola
Bengelsdorf, Frank R.
Jensen, Torbjørn Ø.
Jendresen, Christian B.
Tindall, Brian J.
Daniel, Rolf
Dürre, Peter
Nielsen, Alex T.
author_facet Redl, Stephanie
Poehlein, Anja
Esser, Carola
Bengelsdorf, Frank R.
Jensen, Torbjørn Ø.
Jendresen, Christian B.
Tindall, Brian J.
Daniel, Rolf
Dürre, Peter
Nielsen, Alex T.
author_sort Redl, Stephanie
collection PubMed
description Fermentation of gases provides a promising opportunity for the production of biochemicals from renewable resources, which has resulted in a growing interest in acetogenic bacteria. Thermophilic organisms provide potential advantages for the fermentation of, e.g., syngas into for example volatile compounds, and the thermophiles Moorella thermoacetica and Moorella thermoautotrophica have become model organisms of acetogenic metabolism. The justification for the recognition of the closely related species M. thermoautotrophica has, however, recently been disputed. In order to expand knowledge on the genus, we have here genome sequenced a total of 12 different M. thermoacetica and M. thermoautotrophica strains. From the sequencing results, it became clear that M. thermoautotrophica DSM 1974(T) consists of at least two different strains. Two different strains were isolated in Lyngby and Ulm from a DSM 1974(T) culture obtained from the DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswick, Germany). Phylogenetic analysis revealed a close relationship between all the sequenced genomes, suggesting that the two strains detected in the type strain of the species M. thermoautotrophica could not be distinguished at the species level from M. thermoacetica. Despite genetic similarities, differences in genomic features were observed between the strains. Differences in compounds that can serve as carbon and energy sources for selected strains were also identified. On the contrary, strain DSM 21394, currently still named M. thermoacetica, obviously represents a new Moorella species. In addition, based on genome analysis and comparison M. glycerini NMP, M. stamsii DSM 26217(T), and M. perchloratireducens An10 cannot be distinguished at the species level. Thus, this comprehensive analysis provides a significantly increased knowledge of the genetic diversity of Moorella strains.
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spelling pubmed-69786392020-02-01 Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica Redl, Stephanie Poehlein, Anja Esser, Carola Bengelsdorf, Frank R. Jensen, Torbjørn Ø. Jendresen, Christian B. Tindall, Brian J. Daniel, Rolf Dürre, Peter Nielsen, Alex T. Front Microbiol Microbiology Fermentation of gases provides a promising opportunity for the production of biochemicals from renewable resources, which has resulted in a growing interest in acetogenic bacteria. Thermophilic organisms provide potential advantages for the fermentation of, e.g., syngas into for example volatile compounds, and the thermophiles Moorella thermoacetica and Moorella thermoautotrophica have become model organisms of acetogenic metabolism. The justification for the recognition of the closely related species M. thermoautotrophica has, however, recently been disputed. In order to expand knowledge on the genus, we have here genome sequenced a total of 12 different M. thermoacetica and M. thermoautotrophica strains. From the sequencing results, it became clear that M. thermoautotrophica DSM 1974(T) consists of at least two different strains. Two different strains were isolated in Lyngby and Ulm from a DSM 1974(T) culture obtained from the DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswick, Germany). Phylogenetic analysis revealed a close relationship between all the sequenced genomes, suggesting that the two strains detected in the type strain of the species M. thermoautotrophica could not be distinguished at the species level from M. thermoacetica. Despite genetic similarities, differences in genomic features were observed between the strains. Differences in compounds that can serve as carbon and energy sources for selected strains were also identified. On the contrary, strain DSM 21394, currently still named M. thermoacetica, obviously represents a new Moorella species. In addition, based on genome analysis and comparison M. glycerini NMP, M. stamsii DSM 26217(T), and M. perchloratireducens An10 cannot be distinguished at the species level. Thus, this comprehensive analysis provides a significantly increased knowledge of the genetic diversity of Moorella strains. Frontiers Media S.A. 2020-01-17 /pmc/articles/PMC6978639/ /pubmed/32010113 http://dx.doi.org/10.3389/fmicb.2019.03070 Text en Copyright © 2020 Redl, Poehlein, Esser, Bengelsdorf, Jensen, Jendresen, Tindall, Daniel, Dürre and Nielsen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Redl, Stephanie
Poehlein, Anja
Esser, Carola
Bengelsdorf, Frank R.
Jensen, Torbjørn Ø.
Jendresen, Christian B.
Tindall, Brian J.
Daniel, Rolf
Dürre, Peter
Nielsen, Alex T.
Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica
title Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica
title_full Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica
title_fullStr Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica
title_full_unstemmed Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica
title_short Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica
title_sort genome-based comparison of all species of the genus moorella, and status of the species moorella thermoacetica and moorella thermoautotrophica
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978639/
https://www.ncbi.nlm.nih.gov/pubmed/32010113
http://dx.doi.org/10.3389/fmicb.2019.03070
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