Cargando…
Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii
BACKGROUND: The ureolytic bacterium Sporosarcina pasteurii is well-known for its capability of microbially induced calcite precipitation (MICP), representing a great potential in constructional engineering and material applications. However, the molecular mechanism for its biomineralization remains...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6979283/ https://www.ncbi.nlm.nih.gov/pubmed/31973723 http://dx.doi.org/10.1186/s12934-020-1281-z |
_version_ | 1783490866879397888 |
---|---|
author | Ma, Liang Pang, Ai-Ping Luo, Yongsheng Lu, Xiaolin Lin, Fengming |
author_facet | Ma, Liang Pang, Ai-Ping Luo, Yongsheng Lu, Xiaolin Lin, Fengming |
author_sort | Ma, Liang |
collection | PubMed |
description | BACKGROUND: The ureolytic bacterium Sporosarcina pasteurii is well-known for its capability of microbially induced calcite precipitation (MICP), representing a great potential in constructional engineering and material applications. However, the molecular mechanism for its biomineralization remains unresolved, as few studies were carried out. RESULTS: The addition of urea into the culture medium provided an alkaline environment that is suitable for S. pasteurii. As compared to S. pasteurii cultivated without urea, S. pasteurii grown with urea showed faster growth and urease production, better shape, more negative surface charge and higher biomineralization ability. To survive the unfavorable growth environment due to the absence of urea, S. pasteurii up-regulated the expression of genes involved in urease production, ATPase synthesis and flagella, possibly occupying resources that can be deployed for MICP. As compared to non-mineralizing bacteria, S. pasteurii exhibited more negative cell surface charge for binding calcium ions and more robust cell structure as nucleation sites. During MICP process, the genes for ATPase synthesis in S. pasteurii was up-regulated while genes for urease production were unchanged. Interestingly, genes involved in flagella were down-regulated during MICP, which might lead to poor mobility of S. pasteurii. Meanwhile, genes in fatty acid degradation pathway were inhibited to maintain the intact cell structure found in calcite precipitation. Both weak mobility and intact cell structure are advantageous for S. pasteurii to serve as nucleation sites during MICP. CONCLUSIONS: Four factors are demonstrated to benefit the super performance of S. pasteurii in MICP. First, the good correlation of biomass growth and urease production of S. pasteurii provides sufficient biomass and urease simultaneously for improved biomineralization. Second, the highly negative cell surface charge of S. pasteurii is good for binding calcium ions. Third, the robust cell structure and fourth, the weak mobility, are key for S. pasteurii to be nucleation sites during MICP. |
format | Online Article Text |
id | pubmed-6979283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69792832020-01-29 Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii Ma, Liang Pang, Ai-Ping Luo, Yongsheng Lu, Xiaolin Lin, Fengming Microb Cell Fact Research BACKGROUND: The ureolytic bacterium Sporosarcina pasteurii is well-known for its capability of microbially induced calcite precipitation (MICP), representing a great potential in constructional engineering and material applications. However, the molecular mechanism for its biomineralization remains unresolved, as few studies were carried out. RESULTS: The addition of urea into the culture medium provided an alkaline environment that is suitable for S. pasteurii. As compared to S. pasteurii cultivated without urea, S. pasteurii grown with urea showed faster growth and urease production, better shape, more negative surface charge and higher biomineralization ability. To survive the unfavorable growth environment due to the absence of urea, S. pasteurii up-regulated the expression of genes involved in urease production, ATPase synthesis and flagella, possibly occupying resources that can be deployed for MICP. As compared to non-mineralizing bacteria, S. pasteurii exhibited more negative cell surface charge for binding calcium ions and more robust cell structure as nucleation sites. During MICP process, the genes for ATPase synthesis in S. pasteurii was up-regulated while genes for urease production were unchanged. Interestingly, genes involved in flagella were down-regulated during MICP, which might lead to poor mobility of S. pasteurii. Meanwhile, genes in fatty acid degradation pathway were inhibited to maintain the intact cell structure found in calcite precipitation. Both weak mobility and intact cell structure are advantageous for S. pasteurii to serve as nucleation sites during MICP. CONCLUSIONS: Four factors are demonstrated to benefit the super performance of S. pasteurii in MICP. First, the good correlation of biomass growth and urease production of S. pasteurii provides sufficient biomass and urease simultaneously for improved biomineralization. Second, the highly negative cell surface charge of S. pasteurii is good for binding calcium ions. Third, the robust cell structure and fourth, the weak mobility, are key for S. pasteurii to be nucleation sites during MICP. BioMed Central 2020-01-23 /pmc/articles/PMC6979283/ /pubmed/31973723 http://dx.doi.org/10.1186/s12934-020-1281-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Ma, Liang Pang, Ai-Ping Luo, Yongsheng Lu, Xiaolin Lin, Fengming Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
title | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
title_full | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
title_fullStr | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
title_full_unstemmed | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
title_short | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
title_sort | beneficial factors for biomineralization by ureolytic bacterium sporosarcina pasteurii |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6979283/ https://www.ncbi.nlm.nih.gov/pubmed/31973723 http://dx.doi.org/10.1186/s12934-020-1281-z |
work_keys_str_mv | AT maliang beneficialfactorsforbiomineralizationbyureolyticbacteriumsporosarcinapasteurii AT pangaiping beneficialfactorsforbiomineralizationbyureolyticbacteriumsporosarcinapasteurii AT luoyongsheng beneficialfactorsforbiomineralizationbyureolyticbacteriumsporosarcinapasteurii AT luxiaolin beneficialfactorsforbiomineralizationbyureolyticbacteriumsporosarcinapasteurii AT linfengming beneficialfactorsforbiomineralizationbyureolyticbacteriumsporosarcinapasteurii |