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The virome of HPV-positive tonsil squamous cell carcinoma and neck metastasis

Oropharyngeal squamous cell carcinoma (OPSCC) represents the most common HPV-related malignancy in the United States with increasing incidence. There is heterogeneity between the behavior and response to treatment of HPV-positive oropharyngeal squamous cell carcinoma that may be linked to the tumor...

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Autores principales: Carey, Ryan M., Rajasekaran, Karthik, Seckar, Tyler, Lin, Xiang, Wei, Zhi, Tong, Charles C.L., Ranasinghe, Viran J., Newman, Jason G., O'Malley, Bert W., Weinstein, Gregory S., Feldman, Michael D., Robertson, Erle S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6980631/
https://www.ncbi.nlm.nih.gov/pubmed/32076488
http://dx.doi.org/10.18632/oncotarget.27436
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author Carey, Ryan M.
Rajasekaran, Karthik
Seckar, Tyler
Lin, Xiang
Wei, Zhi
Tong, Charles C.L.
Ranasinghe, Viran J.
Newman, Jason G.
O'Malley, Bert W.
Weinstein, Gregory S.
Feldman, Michael D.
Robertson, Erle S.
author_facet Carey, Ryan M.
Rajasekaran, Karthik
Seckar, Tyler
Lin, Xiang
Wei, Zhi
Tong, Charles C.L.
Ranasinghe, Viran J.
Newman, Jason G.
O'Malley, Bert W.
Weinstein, Gregory S.
Feldman, Michael D.
Robertson, Erle S.
author_sort Carey, Ryan M.
collection PubMed
description Oropharyngeal squamous cell carcinoma (OPSCC) represents the most common HPV-related malignancy in the United States with increasing incidence. There is heterogeneity between the behavior and response to treatment of HPV-positive oropharyngeal squamous cell carcinoma that may be linked to the tumor virome. In this prospective study, a pan-pathogen microarray (PathoChip) was used to determine the virome of early stage, p16-positive OPSCC and neck metastasis treated with transoral robotic surgery (TORS) and neck dissection. The virome findings of primary tumors and neck lymph nodes were correlated with clinical data to determine if specific organisms were associated with clinical outcomes. A total of 114 patients were enrolled in the study. Double-stranded DNA viruses, specifically Papillomaviridae, showed the highest hybridization signal (viral copies) across all viral families in the primary and positive lymph node samples. High hybridization signals were also detected for signatures of Baculoviridae, Reoviridae, Siphoviridae, Myoviridae, and Polydnaviridae in most of the cancer specimens, including the lymph nodes without cancer present. Across all HPV signatures, HPV16 and 18 had the highest average hybridization signal index and prevalence. To our knowledge, this is the first study that has identified the viral signatures of OPSCC tumors. This will serve as a foundation for future research investigating the role of the virome in OPSCC. Further investigation into the OPSCC microbiome and its variations may allow for improved appreciation of the impact of microbial dysbiosis on risk stratification, oncologic outcomes, and treatment response which has been shown in other cancer sites.
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spelling pubmed-69806312020-02-19 The virome of HPV-positive tonsil squamous cell carcinoma and neck metastasis Carey, Ryan M. Rajasekaran, Karthik Seckar, Tyler Lin, Xiang Wei, Zhi Tong, Charles C.L. Ranasinghe, Viran J. Newman, Jason G. O'Malley, Bert W. Weinstein, Gregory S. Feldman, Michael D. Robertson, Erle S. Oncotarget Research Paper Oropharyngeal squamous cell carcinoma (OPSCC) represents the most common HPV-related malignancy in the United States with increasing incidence. There is heterogeneity between the behavior and response to treatment of HPV-positive oropharyngeal squamous cell carcinoma that may be linked to the tumor virome. In this prospective study, a pan-pathogen microarray (PathoChip) was used to determine the virome of early stage, p16-positive OPSCC and neck metastasis treated with transoral robotic surgery (TORS) and neck dissection. The virome findings of primary tumors and neck lymph nodes were correlated with clinical data to determine if specific organisms were associated with clinical outcomes. A total of 114 patients were enrolled in the study. Double-stranded DNA viruses, specifically Papillomaviridae, showed the highest hybridization signal (viral copies) across all viral families in the primary and positive lymph node samples. High hybridization signals were also detected for signatures of Baculoviridae, Reoviridae, Siphoviridae, Myoviridae, and Polydnaviridae in most of the cancer specimens, including the lymph nodes without cancer present. Across all HPV signatures, HPV16 and 18 had the highest average hybridization signal index and prevalence. To our knowledge, this is the first study that has identified the viral signatures of OPSCC tumors. This will serve as a foundation for future research investigating the role of the virome in OPSCC. Further investigation into the OPSCC microbiome and its variations may allow for improved appreciation of the impact of microbial dysbiosis on risk stratification, oncologic outcomes, and treatment response which has been shown in other cancer sites. Impact Journals LLC 2020-01-21 /pmc/articles/PMC6980631/ /pubmed/32076488 http://dx.doi.org/10.18632/oncotarget.27436 Text en Copyright: © 2020 Carey et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Carey, Ryan M.
Rajasekaran, Karthik
Seckar, Tyler
Lin, Xiang
Wei, Zhi
Tong, Charles C.L.
Ranasinghe, Viran J.
Newman, Jason G.
O'Malley, Bert W.
Weinstein, Gregory S.
Feldman, Michael D.
Robertson, Erle S.
The virome of HPV-positive tonsil squamous cell carcinoma and neck metastasis
title The virome of HPV-positive tonsil squamous cell carcinoma and neck metastasis
title_full The virome of HPV-positive tonsil squamous cell carcinoma and neck metastasis
title_fullStr The virome of HPV-positive tonsil squamous cell carcinoma and neck metastasis
title_full_unstemmed The virome of HPV-positive tonsil squamous cell carcinoma and neck metastasis
title_short The virome of HPV-positive tonsil squamous cell carcinoma and neck metastasis
title_sort virome of hpv-positive tonsil squamous cell carcinoma and neck metastasis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6980631/
https://www.ncbi.nlm.nih.gov/pubmed/32076488
http://dx.doi.org/10.18632/oncotarget.27436
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