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Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluo...

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Autores principales: Gómez-Varela, Ana I., Stamov, Dimitar R., Miranda, Adelaide, Alves, Rosana, Barata-Antunes, Cláudia, Dambournet, Daphné, Drubin, David G., Paiva, Sandra, De Beule, Pieter A. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981207/
https://www.ncbi.nlm.nih.gov/pubmed/31980680
http://dx.doi.org/10.1038/s41598-020-57885-z
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author Gómez-Varela, Ana I.
Stamov, Dimitar R.
Miranda, Adelaide
Alves, Rosana
Barata-Antunes, Cláudia
Dambournet, Daphné
Drubin, David G.
Paiva, Sandra
De Beule, Pieter A. A.
author_facet Gómez-Varela, Ana I.
Stamov, Dimitar R.
Miranda, Adelaide
Alves, Rosana
Barata-Antunes, Cláudia
Dambournet, Daphné
Drubin, David G.
Paiva, Sandra
De Beule, Pieter A. A.
author_sort Gómez-Varela, Ana I.
collection PubMed
description Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal.
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spelling pubmed-69812072020-01-30 Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences Gómez-Varela, Ana I. Stamov, Dimitar R. Miranda, Adelaide Alves, Rosana Barata-Antunes, Cláudia Dambournet, Daphné Drubin, David G. Paiva, Sandra De Beule, Pieter A. A. Sci Rep Article Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal. Nature Publishing Group UK 2020-01-24 /pmc/articles/PMC6981207/ /pubmed/31980680 http://dx.doi.org/10.1038/s41598-020-57885-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gómez-Varela, Ana I.
Stamov, Dimitar R.
Miranda, Adelaide
Alves, Rosana
Barata-Antunes, Cláudia
Dambournet, Daphné
Drubin, David G.
Paiva, Sandra
De Beule, Pieter A. A.
Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
title Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
title_full Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
title_fullStr Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
title_full_unstemmed Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
title_short Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
title_sort simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined sim and afm platform for the life sciences
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981207/
https://www.ncbi.nlm.nih.gov/pubmed/31980680
http://dx.doi.org/10.1038/s41598-020-57885-z
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