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Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA
MicroRNAs (miRNAs) are new potential biomarkers for early diagnosis and classification of cancer. This study is the first attempt to use biocatalytic amplification reactions combined with capillary electrophoresis to detect multiple miRNAs simultaneously. In this way, miRNAs, as catalysts, can catal...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981575/ https://www.ncbi.nlm.nih.gov/pubmed/31861744 http://dx.doi.org/10.3390/ijms21010051 |
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author | Hu, Ruibin Chen, Yi |
author_facet | Hu, Ruibin Chen, Yi |
author_sort | Hu, Ruibin |
collection | PubMed |
description | MicroRNAs (miRNAs) are new potential biomarkers for early diagnosis and classification of cancer. This study is the first attempt to use biocatalytic amplification reactions combined with capillary electrophoresis to detect multiple miRNAs simultaneously. In this way, miRNAs, as catalysts, can catalyze two single strands of DNA to form double-strand DNA. Feasibility was demonstrated by non-gel capillary electrophoresis coupled with UV detection (NGCE-UV). The detection limit was improved down to 1.0 nM, having ca. 10(3)-fold improvement. This method has a good linear range of between 3.0 nM and 300 nM, with R(2) at 0.99, recovery at 88–115%, and peak area precision at 1–12.7%. Using three target miRNAs as a model can achieve the baseline separation and good selectivity. The proposed biocatalysis coupled with a capillary electrophoresis-based method is simple, rapid, multiplexed, and cost-effective, making it potentially applicable for simultaneous, large-scale screening for other nucleic acids biomarkers and related research. |
format | Online Article Text |
id | pubmed-6981575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69815752020-02-03 Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA Hu, Ruibin Chen, Yi Int J Mol Sci Article MicroRNAs (miRNAs) are new potential biomarkers for early diagnosis and classification of cancer. This study is the first attempt to use biocatalytic amplification reactions combined with capillary electrophoresis to detect multiple miRNAs simultaneously. In this way, miRNAs, as catalysts, can catalyze two single strands of DNA to form double-strand DNA. Feasibility was demonstrated by non-gel capillary electrophoresis coupled with UV detection (NGCE-UV). The detection limit was improved down to 1.0 nM, having ca. 10(3)-fold improvement. This method has a good linear range of between 3.0 nM and 300 nM, with R(2) at 0.99, recovery at 88–115%, and peak area precision at 1–12.7%. Using three target miRNAs as a model can achieve the baseline separation and good selectivity. The proposed biocatalysis coupled with a capillary electrophoresis-based method is simple, rapid, multiplexed, and cost-effective, making it potentially applicable for simultaneous, large-scale screening for other nucleic acids biomarkers and related research. MDPI 2019-12-19 /pmc/articles/PMC6981575/ /pubmed/31861744 http://dx.doi.org/10.3390/ijms21010051 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hu, Ruibin Chen, Yi Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA |
title | Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA |
title_full | Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA |
title_fullStr | Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA |
title_full_unstemmed | Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA |
title_short | Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA |
title_sort | biocatalytic amplification of uv signal in capillary electrophoresis of microrna |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981575/ https://www.ncbi.nlm.nih.gov/pubmed/31861744 http://dx.doi.org/10.3390/ijms21010051 |
work_keys_str_mv | AT huruibin biocatalyticamplificationofuvsignalincapillaryelectrophoresisofmicrorna AT chenyi biocatalyticamplificationofuvsignalincapillaryelectrophoresisofmicrorna |