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Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions

APETALA2/ETHYLENE RESPONSE FACTOR transcription factors (AP2/ERFs) play crucial roles in adaptation to stresses such as those caused by pathogens, wounding and cold. Although their name suggests a specific role in ethylene signalling, some ERF members also co-ordinate signals regulated by other key...

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Autores principales: Sun, Xiaolin, Malhis, Nawar, Zhao, Bi, Xue, Bin, Gsponer, Joerg, Rikkerink, Erik H. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981732/
https://www.ncbi.nlm.nih.gov/pubmed/31861935
http://dx.doi.org/10.3390/ijms21010074
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author Sun, Xiaolin
Malhis, Nawar
Zhao, Bi
Xue, Bin
Gsponer, Joerg
Rikkerink, Erik H. A.
author_facet Sun, Xiaolin
Malhis, Nawar
Zhao, Bi
Xue, Bin
Gsponer, Joerg
Rikkerink, Erik H. A.
author_sort Sun, Xiaolin
collection PubMed
description APETALA2/ETHYLENE RESPONSE FACTOR transcription factors (AP2/ERFs) play crucial roles in adaptation to stresses such as those caused by pathogens, wounding and cold. Although their name suggests a specific role in ethylene signalling, some ERF members also co-ordinate signals regulated by other key plant stress hormones such as jasmonate, abscisic acid and salicylate. We analysed a set of ERF proteins from three divergent plant species for intrinsically disorder regions containing conserved segments involved in protein–protein interaction known as Molecular Recognition Features (MoRFs). Then we correlated the MoRFs identified with a number of known functional features where these could be identified. Our analyses suggest that MoRFs, with plasticity in their disordered surroundings, are highly functional and may have been shuffled between related protein families driven by selection. A particularly important role may be played by the alpha helical component of the structured DNA binding domain to permit specificity. We also present examples of computationally identified MoRFs that have no known function and provide a valuable conceptual framework to link both disordered and ordered structural features within this family to diverse function.
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spelling pubmed-69817322020-02-07 Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions Sun, Xiaolin Malhis, Nawar Zhao, Bi Xue, Bin Gsponer, Joerg Rikkerink, Erik H. A. Int J Mol Sci Article APETALA2/ETHYLENE RESPONSE FACTOR transcription factors (AP2/ERFs) play crucial roles in adaptation to stresses such as those caused by pathogens, wounding and cold. Although their name suggests a specific role in ethylene signalling, some ERF members also co-ordinate signals regulated by other key plant stress hormones such as jasmonate, abscisic acid and salicylate. We analysed a set of ERF proteins from three divergent plant species for intrinsically disorder regions containing conserved segments involved in protein–protein interaction known as Molecular Recognition Features (MoRFs). Then we correlated the MoRFs identified with a number of known functional features where these could be identified. Our analyses suggest that MoRFs, with plasticity in their disordered surroundings, are highly functional and may have been shuffled between related protein families driven by selection. A particularly important role may be played by the alpha helical component of the structured DNA binding domain to permit specificity. We also present examples of computationally identified MoRFs that have no known function and provide a valuable conceptual framework to link both disordered and ordered structural features within this family to diverse function. MDPI 2019-12-20 /pmc/articles/PMC6981732/ /pubmed/31861935 http://dx.doi.org/10.3390/ijms21010074 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Xiaolin
Malhis, Nawar
Zhao, Bi
Xue, Bin
Gsponer, Joerg
Rikkerink, Erik H. A.
Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions
title Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions
title_full Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions
title_fullStr Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions
title_full_unstemmed Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions
title_short Computational Disorder Analysis in Ethylene Response Factors Uncovers Binding Motifs Critical to Their Diverse Functions
title_sort computational disorder analysis in ethylene response factors uncovers binding motifs critical to their diverse functions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981732/
https://www.ncbi.nlm.nih.gov/pubmed/31861935
http://dx.doi.org/10.3390/ijms21010074
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