Cargando…

Co-Expression Profile of TNF Membrane-Bound Receptors Type 1 and 2 in Rheumatoid Arthritis on Immunocompetent Cells Subsets

Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, and by number, density, and co-expression of its membrane-bound receptor...

Descripción completa

Detalles Bibliográficos
Autores principales: Alshevskaya, Alina, Lopatnikova, Julia, Zhukova, Julia, Chumasova, Oksana, Shkaruba, Nadezhda, Sizikov, Aleksey, Evsegneeva, Irina, Gladkikh, Victor, Karaulov, Aleksander, Sennikov, Sergey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982057/
https://www.ncbi.nlm.nih.gov/pubmed/31906244
http://dx.doi.org/10.3390/ijms21010288
_version_ 1783491227480489984
author Alshevskaya, Alina
Lopatnikova, Julia
Zhukova, Julia
Chumasova, Oksana
Shkaruba, Nadezhda
Sizikov, Aleksey
Evsegneeva, Irina
Gladkikh, Victor
Karaulov, Aleksander
Sennikov, Sergey V.
author_facet Alshevskaya, Alina
Lopatnikova, Julia
Zhukova, Julia
Chumasova, Oksana
Shkaruba, Nadezhda
Sizikov, Aleksey
Evsegneeva, Irina
Gladkikh, Victor
Karaulov, Aleksander
Sennikov, Sergey V.
author_sort Alshevskaya, Alina
collection PubMed
description Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, and by number, density, and co-expression of its membrane-bound receptors type 1 and 2 (TNFR1 and TNFR2). The aim of this study was to reveal associations between TNFR1/2 co-expression profile parameters and RA disease activity indicators. Methods: PBMC were analyzed from 46 healthy donors and 64 patients with RA using flow cytometry. Patients were divided according to the disease activity score (DAS) 28 index into groups with high (n = 22, 34.4%), moderate (n = 30, 46.9%), and low (n = 12, 18.8%) disease activity. Co-expression of TNFR1 and TNFR2 was studied by evaluating the percentage of cells, with different receptors, and by counting the number of receptors of each type per cell, using QuantiBritePE beads. Associations between disease severity and activity indicators and parameters of TNFα receptor expression in subpopulations of immune cells were studied. Results: T cell subsets from RA patients were characterized by co-expression of TNFR1 and TNFR2, and were found to differ significantly compared with healthy donors. Memory cells both among T helper cells and cytotoxic T cells demonstrated the most significant differences in TNFR-expression profile. Multivariable logistic regression revealed model to identified RA patients from healthy individual based on the TNFR1/2 co-expression parameters. Conclusion: The profile of TNFR1\2 co-expression differs in RA comparing with health. Proportion of TNFR1+TNFR2- cells increased significantly among memory T helper cells and activated cytotoxic T cells, and decreased significantly among naïve cytotoxic T cells and T regulatory cells as compared with health. The parameters of TNFR1\2 co-expression in RA are associated with clinical and laboratory indicators of disease activity.
format Online
Article
Text
id pubmed-6982057
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69820572020-02-07 Co-Expression Profile of TNF Membrane-Bound Receptors Type 1 and 2 in Rheumatoid Arthritis on Immunocompetent Cells Subsets Alshevskaya, Alina Lopatnikova, Julia Zhukova, Julia Chumasova, Oksana Shkaruba, Nadezhda Sizikov, Aleksey Evsegneeva, Irina Gladkikh, Victor Karaulov, Aleksander Sennikov, Sergey V. Int J Mol Sci Article Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, and by number, density, and co-expression of its membrane-bound receptors type 1 and 2 (TNFR1 and TNFR2). The aim of this study was to reveal associations between TNFR1/2 co-expression profile parameters and RA disease activity indicators. Methods: PBMC were analyzed from 46 healthy donors and 64 patients with RA using flow cytometry. Patients were divided according to the disease activity score (DAS) 28 index into groups with high (n = 22, 34.4%), moderate (n = 30, 46.9%), and low (n = 12, 18.8%) disease activity. Co-expression of TNFR1 and TNFR2 was studied by evaluating the percentage of cells, with different receptors, and by counting the number of receptors of each type per cell, using QuantiBritePE beads. Associations between disease severity and activity indicators and parameters of TNFα receptor expression in subpopulations of immune cells were studied. Results: T cell subsets from RA patients were characterized by co-expression of TNFR1 and TNFR2, and were found to differ significantly compared with healthy donors. Memory cells both among T helper cells and cytotoxic T cells demonstrated the most significant differences in TNFR-expression profile. Multivariable logistic regression revealed model to identified RA patients from healthy individual based on the TNFR1/2 co-expression parameters. Conclusion: The profile of TNFR1\2 co-expression differs in RA comparing with health. Proportion of TNFR1+TNFR2- cells increased significantly among memory T helper cells and activated cytotoxic T cells, and decreased significantly among naïve cytotoxic T cells and T regulatory cells as compared with health. The parameters of TNFR1\2 co-expression in RA are associated with clinical and laboratory indicators of disease activity. MDPI 2019-12-31 /pmc/articles/PMC6982057/ /pubmed/31906244 http://dx.doi.org/10.3390/ijms21010288 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alshevskaya, Alina
Lopatnikova, Julia
Zhukova, Julia
Chumasova, Oksana
Shkaruba, Nadezhda
Sizikov, Aleksey
Evsegneeva, Irina
Gladkikh, Victor
Karaulov, Aleksander
Sennikov, Sergey V.
Co-Expression Profile of TNF Membrane-Bound Receptors Type 1 and 2 in Rheumatoid Arthritis on Immunocompetent Cells Subsets
title Co-Expression Profile of TNF Membrane-Bound Receptors Type 1 and 2 in Rheumatoid Arthritis on Immunocompetent Cells Subsets
title_full Co-Expression Profile of TNF Membrane-Bound Receptors Type 1 and 2 in Rheumatoid Arthritis on Immunocompetent Cells Subsets
title_fullStr Co-Expression Profile of TNF Membrane-Bound Receptors Type 1 and 2 in Rheumatoid Arthritis on Immunocompetent Cells Subsets
title_full_unstemmed Co-Expression Profile of TNF Membrane-Bound Receptors Type 1 and 2 in Rheumatoid Arthritis on Immunocompetent Cells Subsets
title_short Co-Expression Profile of TNF Membrane-Bound Receptors Type 1 and 2 in Rheumatoid Arthritis on Immunocompetent Cells Subsets
title_sort co-expression profile of tnf membrane-bound receptors type 1 and 2 in rheumatoid arthritis on immunocompetent cells subsets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982057/
https://www.ncbi.nlm.nih.gov/pubmed/31906244
http://dx.doi.org/10.3390/ijms21010288
work_keys_str_mv AT alshevskayaalina coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets
AT lopatnikovajulia coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets
AT zhukovajulia coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets
AT chumasovaoksana coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets
AT shkarubanadezhda coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets
AT sizikovaleksey coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets
AT evsegneevairina coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets
AT gladkikhvictor coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets
AT karaulovaleksander coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets
AT sennikovsergeyv coexpressionprofileoftnfmembraneboundreceptorstype1and2inrheumatoidarthritisonimmunocompetentcellssubsets