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Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa)
Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes of upland and lowland rice (Oryza sativa L. ssp. japonica and indica) to drought stress at the late vegetative stage, and harvested leaves for label-free shotgun proteomic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982093/ https://www.ncbi.nlm.nih.gov/pubmed/31935846 http://dx.doi.org/10.3390/ijms21010363 |
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author | Hamzelou, Sara Pascovici, Dana Kamath, Karthik Shantharam Amirkhani, Ardeshir McKay, Matthew Mirzaei, Mehdi Atwell, Brian J. Haynes, Paul A. |
author_facet | Hamzelou, Sara Pascovici, Dana Kamath, Karthik Shantharam Amirkhani, Ardeshir McKay, Matthew Mirzaei, Mehdi Atwell, Brian J. Haynes, Paul A. |
author_sort | Hamzelou, Sara |
collection | PubMed |
description | Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes of upland and lowland rice (Oryza sativa L. ssp. japonica and indica) to drought stress at the late vegetative stage, and harvested leaves for label-free shotgun proteomics. Gene ontology analysis was used to identify common drought-responsive proteins in vegetative tissues, and leaf proteins that are unique to individual genotypes, suggesting diversity in the metabolic responses to drought. Eight proteins were found to be induced in response to drought stress in all eight genotypes. A total of 213 proteins were identified in a single genotype, 83 of which were increased in abundance in response to drought stress. In total, 10 of these 83 proteins were of a largely uncharacterized function, making them candidates for functional analysis and potential biomarkers for drought tolerance. |
format | Online Article Text |
id | pubmed-6982093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69820932020-02-07 Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa) Hamzelou, Sara Pascovici, Dana Kamath, Karthik Shantharam Amirkhani, Ardeshir McKay, Matthew Mirzaei, Mehdi Atwell, Brian J. Haynes, Paul A. Int J Mol Sci Article Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes of upland and lowland rice (Oryza sativa L. ssp. japonica and indica) to drought stress at the late vegetative stage, and harvested leaves for label-free shotgun proteomics. Gene ontology analysis was used to identify common drought-responsive proteins in vegetative tissues, and leaf proteins that are unique to individual genotypes, suggesting diversity in the metabolic responses to drought. Eight proteins were found to be induced in response to drought stress in all eight genotypes. A total of 213 proteins were identified in a single genotype, 83 of which were increased in abundance in response to drought stress. In total, 10 of these 83 proteins were of a largely uncharacterized function, making them candidates for functional analysis and potential biomarkers for drought tolerance. MDPI 2020-01-06 /pmc/articles/PMC6982093/ /pubmed/31935846 http://dx.doi.org/10.3390/ijms21010363 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hamzelou, Sara Pascovici, Dana Kamath, Karthik Shantharam Amirkhani, Ardeshir McKay, Matthew Mirzaei, Mehdi Atwell, Brian J. Haynes, Paul A. Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa) |
title | Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa) |
title_full | Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa) |
title_fullStr | Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa) |
title_full_unstemmed | Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa) |
title_short | Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa) |
title_sort | proteomic responses to drought vary widely among eight diverse genotypes of rice (oryza sativa) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982093/ https://www.ncbi.nlm.nih.gov/pubmed/31935846 http://dx.doi.org/10.3390/ijms21010363 |
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