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The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis

Vascular smooth muscle cells (VSMCs) in the arterial wall have diverse functions. In pathological states, the interplay between transcripts and microRNAs (miRNAs) leads to phenotypic changes. Understanding the regulatory role of miRNAs and their target genes may reveal how VSMCs modulate the pathoge...

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Autores principales: Woo, Chin Cheng, Liu, Wenting, Lin, Xiao Yun, Dorajoo, Rajkumar, Lee, Kee Wah, Richards, A Mark, Lee, Chuen Neng, Wongsurawat, Thidathip, Nookaew, Intawat, Sorokin, Vitaly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982107/
https://www.ncbi.nlm.nih.gov/pubmed/31861407
http://dx.doi.org/10.3390/ijms21010011
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author Woo, Chin Cheng
Liu, Wenting
Lin, Xiao Yun
Dorajoo, Rajkumar
Lee, Kee Wah
Richards, A Mark
Lee, Chuen Neng
Wongsurawat, Thidathip
Nookaew, Intawat
Sorokin, Vitaly
author_facet Woo, Chin Cheng
Liu, Wenting
Lin, Xiao Yun
Dorajoo, Rajkumar
Lee, Kee Wah
Richards, A Mark
Lee, Chuen Neng
Wongsurawat, Thidathip
Nookaew, Intawat
Sorokin, Vitaly
author_sort Woo, Chin Cheng
collection PubMed
description Vascular smooth muscle cells (VSMCs) in the arterial wall have diverse functions. In pathological states, the interplay between transcripts and microRNAs (miRNAs) leads to phenotypic changes. Understanding the regulatory role of miRNAs and their target genes may reveal how VSMCs modulate the pathogenesis of coronary artery disease. Laser capture microdissection was performed on aortic wall tissues obtained from coronary artery bypass graft patients with and without recent acute myocardial infarction (MI). The mSMRT-qPCR miRNA assay platform (MiRXES, Singapore) was used to profile miRNA. The miRNA data were co-analyzed with significant mRNA transcripts. TargetScan 7.1 was applied to evaluate miRNA–mRNA interactions. The miRNA profiles of 29 patients (16 MI and 13 non-MI) were evaluated. Thirteen VSMC-related miRNAs were differentially expressed between the MI and non-MI groups. Analysis revealed seven miRNA-targeted mRNAs related to muscular tissue differentiation and proliferation. TargetScan revealed that among the VSMC-related transcripts, MBNL1 had a recognition site that matched the hsa-miR-30b-5p target seed sequence. In addition to predicted analysis, our experiment in vitro with human VSMC culture confirmed that hsa-miR-30b-5p negatively correlated with MBNL1. Our data showed that overexpression of hsa-miR-30b-5p led to downregulation of MBNL1 in VSMCs. This process influences VSMC proliferation and might be involved in VSMC differentiation.
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spelling pubmed-69821072020-02-07 The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis Woo, Chin Cheng Liu, Wenting Lin, Xiao Yun Dorajoo, Rajkumar Lee, Kee Wah Richards, A Mark Lee, Chuen Neng Wongsurawat, Thidathip Nookaew, Intawat Sorokin, Vitaly Int J Mol Sci Article Vascular smooth muscle cells (VSMCs) in the arterial wall have diverse functions. In pathological states, the interplay between transcripts and microRNAs (miRNAs) leads to phenotypic changes. Understanding the regulatory role of miRNAs and their target genes may reveal how VSMCs modulate the pathogenesis of coronary artery disease. Laser capture microdissection was performed on aortic wall tissues obtained from coronary artery bypass graft patients with and without recent acute myocardial infarction (MI). The mSMRT-qPCR miRNA assay platform (MiRXES, Singapore) was used to profile miRNA. The miRNA data were co-analyzed with significant mRNA transcripts. TargetScan 7.1 was applied to evaluate miRNA–mRNA interactions. The miRNA profiles of 29 patients (16 MI and 13 non-MI) were evaluated. Thirteen VSMC-related miRNAs were differentially expressed between the MI and non-MI groups. Analysis revealed seven miRNA-targeted mRNAs related to muscular tissue differentiation and proliferation. TargetScan revealed that among the VSMC-related transcripts, MBNL1 had a recognition site that matched the hsa-miR-30b-5p target seed sequence. In addition to predicted analysis, our experiment in vitro with human VSMC culture confirmed that hsa-miR-30b-5p negatively correlated with MBNL1. Our data showed that overexpression of hsa-miR-30b-5p led to downregulation of MBNL1 in VSMCs. This process influences VSMC proliferation and might be involved in VSMC differentiation. MDPI 2019-12-18 /pmc/articles/PMC6982107/ /pubmed/31861407 http://dx.doi.org/10.3390/ijms21010011 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Woo, Chin Cheng
Liu, Wenting
Lin, Xiao Yun
Dorajoo, Rajkumar
Lee, Kee Wah
Richards, A Mark
Lee, Chuen Neng
Wongsurawat, Thidathip
Nookaew, Intawat
Sorokin, Vitaly
The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis
title The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis
title_full The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis
title_fullStr The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis
title_full_unstemmed The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis
title_short The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis
title_sort interaction between 30b-5p mirna and mbnl1 mrna is involved in vascular smooth muscle cell differentiation in patients with coronary atherosclerosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982107/
https://www.ncbi.nlm.nih.gov/pubmed/31861407
http://dx.doi.org/10.3390/ijms21010011
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