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Glucocorticoids Equally Stimulate Epithelial Na(+) Transport in Male and Female Fetal Alveolar Cells

Preterm infants frequently suffer from respiratory distress syndrome (RDS), possibly due to lower expression of epithelial Na(+) channels (ENaC). RDS incidence is sex-specific, affecting males almost twice as often. Despite the use of antenatal glucocorticoids (GCs), the sex difference persists. It...

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Autores principales: Laube, Mandy, Riedel, Diana, Ackermann, Benjamin, Haase, Melanie, H. Thome, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982285/
https://www.ncbi.nlm.nih.gov/pubmed/31861781
http://dx.doi.org/10.3390/ijms21010057
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author Laube, Mandy
Riedel, Diana
Ackermann, Benjamin
Haase, Melanie
H. Thome, Ulrich
author_facet Laube, Mandy
Riedel, Diana
Ackermann, Benjamin
Haase, Melanie
H. Thome, Ulrich
author_sort Laube, Mandy
collection PubMed
description Preterm infants frequently suffer from respiratory distress syndrome (RDS), possibly due to lower expression of epithelial Na(+) channels (ENaC). RDS incidence is sex-specific, affecting males almost twice as often. Despite the use of antenatal glucocorticoids (GCs), the sex difference persists. It is still controversial whether both sexes benefit equally from GCs. We previously showed that Na(+) transport is higher in female compared with male fetal distal lung epithelial (FDLE) cells. Since GCs increase Na(+) transport, we hypothesized that their stimulating effect might be sex-specific. We analyzed FDLE cells with Ussing chambers and RT-qPCR in the presence or absence of fetal serum. In serum-free medium, GCs increased the ENaC activity and mRNA expression, independent of sex. In contrast, GCs did not increase the Na(+) transport in serum-supplemented media and abolished the otherwise observed sex difference. Inhibition of the GC receptor in the presence of serum did not equalize Na(+) transport between male and female cells. The GC-induced surfactant protein mRNA expression was concentration and sex-specific. In conclusion, female and male FDLE cells exhibit no sex difference in response to GCs with regard to Na(+) transport, and GR activity does not contribute to the higher Na(+) transport in females.
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spelling pubmed-69822852020-02-07 Glucocorticoids Equally Stimulate Epithelial Na(+) Transport in Male and Female Fetal Alveolar Cells Laube, Mandy Riedel, Diana Ackermann, Benjamin Haase, Melanie H. Thome, Ulrich Int J Mol Sci Article Preterm infants frequently suffer from respiratory distress syndrome (RDS), possibly due to lower expression of epithelial Na(+) channels (ENaC). RDS incidence is sex-specific, affecting males almost twice as often. Despite the use of antenatal glucocorticoids (GCs), the sex difference persists. It is still controversial whether both sexes benefit equally from GCs. We previously showed that Na(+) transport is higher in female compared with male fetal distal lung epithelial (FDLE) cells. Since GCs increase Na(+) transport, we hypothesized that their stimulating effect might be sex-specific. We analyzed FDLE cells with Ussing chambers and RT-qPCR in the presence or absence of fetal serum. In serum-free medium, GCs increased the ENaC activity and mRNA expression, independent of sex. In contrast, GCs did not increase the Na(+) transport in serum-supplemented media and abolished the otherwise observed sex difference. Inhibition of the GC receptor in the presence of serum did not equalize Na(+) transport between male and female cells. The GC-induced surfactant protein mRNA expression was concentration and sex-specific. In conclusion, female and male FDLE cells exhibit no sex difference in response to GCs with regard to Na(+) transport, and GR activity does not contribute to the higher Na(+) transport in females. MDPI 2019-12-20 /pmc/articles/PMC6982285/ /pubmed/31861781 http://dx.doi.org/10.3390/ijms21010057 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Laube, Mandy
Riedel, Diana
Ackermann, Benjamin
Haase, Melanie
H. Thome, Ulrich
Glucocorticoids Equally Stimulate Epithelial Na(+) Transport in Male and Female Fetal Alveolar Cells
title Glucocorticoids Equally Stimulate Epithelial Na(+) Transport in Male and Female Fetal Alveolar Cells
title_full Glucocorticoids Equally Stimulate Epithelial Na(+) Transport in Male and Female Fetal Alveolar Cells
title_fullStr Glucocorticoids Equally Stimulate Epithelial Na(+) Transport in Male and Female Fetal Alveolar Cells
title_full_unstemmed Glucocorticoids Equally Stimulate Epithelial Na(+) Transport in Male and Female Fetal Alveolar Cells
title_short Glucocorticoids Equally Stimulate Epithelial Na(+) Transport in Male and Female Fetal Alveolar Cells
title_sort glucocorticoids equally stimulate epithelial na(+) transport in male and female fetal alveolar cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982285/
https://www.ncbi.nlm.nih.gov/pubmed/31861781
http://dx.doi.org/10.3390/ijms21010057
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