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CD86(+)/CD206(+) tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma

BACKGROUND: As the main cellular ingredients of tumor microenvironment, tumor-associated macrophages (TAMs) play a vital role in tumor development and progression. Recent studies have suggested that TAMs are sensitive and specific prognostic factors in numerous cancers. The primary purpose of this s...

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Autores principales: Sun, Dalong, Luo, Tiancheng, Dong, Pingping, Zhang, Ningping, Chen, Jing, Zhang, Shuncai, Liu, Longzi, Dong, Ling, Zhang, Si
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982414/
https://www.ncbi.nlm.nih.gov/pubmed/32002338
http://dx.doi.org/10.7717/peerj.8458
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author Sun, Dalong
Luo, Tiancheng
Dong, Pingping
Zhang, Ningping
Chen, Jing
Zhang, Shuncai
Liu, Longzi
Dong, Ling
Zhang, Si
author_facet Sun, Dalong
Luo, Tiancheng
Dong, Pingping
Zhang, Ningping
Chen, Jing
Zhang, Shuncai
Liu, Longzi
Dong, Ling
Zhang, Si
author_sort Sun, Dalong
collection PubMed
description BACKGROUND: As the main cellular ingredients of tumor microenvironment, tumor-associated macrophages (TAMs) play a vital role in tumor development and progression. Recent studies have suggested that TAMs are sensitive and specific prognostic factors in numerous cancers. The primary purpose of this study is to determine the prognostic significance of TAMs in intrahepatic cholangiocarcinoma (ICC). METHODS: Immunohistochemical staining of CD68, CD86 and CD206 were performed in tissue microarrays containing 322 patients, who underwent surgical resection and were pathologically diagnosed with ICC. The prognostic value of CD68, CD86 and CD206 were evaluated by Kaplan–Meier analysis (log-rank test) and nomogram models. RESULTS: We demonstrated that the CD86(+)/CD206(+) TAMs model was an independent prognostic index for ICC patients. Patients with low CD86(+) TAMs and high CD206(+) TAMs infiltration had a markedly worse prognosis and increased risk of post-operative recurrence when compared to high CD86(+) TAMs and low CD206(+) TAMs intratumoral infiltration. Furthermore, subgroup analysis indicated that the CD86(+)/CD206(+) TAMs model predicted prognosis of ICC patients more powerfully than single macrophage immunomarker. Interestingly, the CD86(+)/CD206(+) TAMs model could further distinguish prognosis of CA-199 negative ICC patients, who were generally presumed to have a more favorable outcome. In order to further perfect the prognostic value of the CD86(+)/CD206(+) TAMs model, we constructed and validated a postoperative nomogram to predict overall survival and recurrence-free survival time in ICC patients. CONCLUSIONS: These findings indicate that the CD86(+)/CD206(+) TAMs model possess potential value as a novel prognostic indicator for ICC patients.
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spelling pubmed-69824142020-01-30 CD86(+)/CD206(+) tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma Sun, Dalong Luo, Tiancheng Dong, Pingping Zhang, Ningping Chen, Jing Zhang, Shuncai Liu, Longzi Dong, Ling Zhang, Si PeerJ Immunology BACKGROUND: As the main cellular ingredients of tumor microenvironment, tumor-associated macrophages (TAMs) play a vital role in tumor development and progression. Recent studies have suggested that TAMs are sensitive and specific prognostic factors in numerous cancers. The primary purpose of this study is to determine the prognostic significance of TAMs in intrahepatic cholangiocarcinoma (ICC). METHODS: Immunohistochemical staining of CD68, CD86 and CD206 were performed in tissue microarrays containing 322 patients, who underwent surgical resection and were pathologically diagnosed with ICC. The prognostic value of CD68, CD86 and CD206 were evaluated by Kaplan–Meier analysis (log-rank test) and nomogram models. RESULTS: We demonstrated that the CD86(+)/CD206(+) TAMs model was an independent prognostic index for ICC patients. Patients with low CD86(+) TAMs and high CD206(+) TAMs infiltration had a markedly worse prognosis and increased risk of post-operative recurrence when compared to high CD86(+) TAMs and low CD206(+) TAMs intratumoral infiltration. Furthermore, subgroup analysis indicated that the CD86(+)/CD206(+) TAMs model predicted prognosis of ICC patients more powerfully than single macrophage immunomarker. Interestingly, the CD86(+)/CD206(+) TAMs model could further distinguish prognosis of CA-199 negative ICC patients, who were generally presumed to have a more favorable outcome. In order to further perfect the prognostic value of the CD86(+)/CD206(+) TAMs model, we constructed and validated a postoperative nomogram to predict overall survival and recurrence-free survival time in ICC patients. CONCLUSIONS: These findings indicate that the CD86(+)/CD206(+) TAMs model possess potential value as a novel prognostic indicator for ICC patients. PeerJ Inc. 2020-01-22 /pmc/articles/PMC6982414/ /pubmed/32002338 http://dx.doi.org/10.7717/peerj.8458 Text en © 2020 Sun et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Immunology
Sun, Dalong
Luo, Tiancheng
Dong, Pingping
Zhang, Ningping
Chen, Jing
Zhang, Shuncai
Liu, Longzi
Dong, Ling
Zhang, Si
CD86(+)/CD206(+) tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma
title CD86(+)/CD206(+) tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma
title_full CD86(+)/CD206(+) tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma
title_fullStr CD86(+)/CD206(+) tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma
title_full_unstemmed CD86(+)/CD206(+) tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma
title_short CD86(+)/CD206(+) tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma
title_sort cd86(+)/cd206(+) tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982414/
https://www.ncbi.nlm.nih.gov/pubmed/32002338
http://dx.doi.org/10.7717/peerj.8458
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