Cargando…

Protopine Protects Mice against LPS-Induced Acute Kidney Injury by Inhibiting Apoptosis and Inflammation via the TLR4 Signaling Pathway

Corydalis humosa Migo is a traditional Chinese medicine that clears away damp heat, relieves sore. Protopine (PRO) is an alkaloid component isolated from C. humosa Migo. However, the role of protopine in acute kidney injury (AKI) has not yet been reported. This study aims to investigate the effect a...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Beibei, Zeng, Mengnan, Li, Meng, Kan, Yuxuan, Li, Benke, Xu, Ruiqi, Wu, Yuanyuan, Wang, Shengchao, Zheng, Xiaoke, Feng, Weisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982873/
https://www.ncbi.nlm.nih.gov/pubmed/31861525
http://dx.doi.org/10.3390/molecules25010015
_version_ 1783491389236969472
author Zhang, Beibei
Zeng, Mengnan
Li, Meng
Kan, Yuxuan
Li, Benke
Xu, Ruiqi
Wu, Yuanyuan
Wang, Shengchao
Zheng, Xiaoke
Feng, Weisheng
author_facet Zhang, Beibei
Zeng, Mengnan
Li, Meng
Kan, Yuxuan
Li, Benke
Xu, Ruiqi
Wu, Yuanyuan
Wang, Shengchao
Zheng, Xiaoke
Feng, Weisheng
author_sort Zhang, Beibei
collection PubMed
description Corydalis humosa Migo is a traditional Chinese medicine that clears away damp heat, relieves sore. Protopine (PRO) is an alkaloid component isolated from C. humosa Migo. However, the role of protopine in acute kidney injury (AKI) has not yet been reported. This study aims to investigate the effect and mechanism of protopine isolated from C. humosa Migo on lipopolysaccharide (LPS)-induced AKI in mice. Inflammation accumulation was assessed by small animal living imaging. The blood urea nitrogen (BUN), and serum creatinine (Scr) were measured to assess the effects of protopine on renal function in LPS-induced AKI. The levels of tumor necrosis factor (TNF), interleukin-2 (IL-2), interferon-γ (IFN-γ), and (interleukin-10) IL-10 in serum were detected by cytometric bead array. Flow cytometry was used to detect the levels of reactive oxygen species (ROS) in primary kidney cells. The proportions of granulocytes, neutrophils, and macrophages in peripheral blood were examined to evaluate the effect of protopine on immune cells in mice with AKI. Toll-like receptor (TLR4) and apoptotic signaling pathway were detected by Western blot analysis. The results showed that protopine markedly improved the renal function, relieve inflammation, reversed inflammatory cytokines, transformed apoptosis markers, and regulated the TLR4 signaling pathway in mice with AKI induced by LPS. The protopine isolated from C. humosa Migo protected mice against LPS-induced AKI by inhibiting apoptosis and inflammation via the TLR4 signaling pathway, thus providing a molecular basis for a novel medical treatment of AKI.
format Online
Article
Text
id pubmed-6982873
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69828732020-02-06 Protopine Protects Mice against LPS-Induced Acute Kidney Injury by Inhibiting Apoptosis and Inflammation via the TLR4 Signaling Pathway Zhang, Beibei Zeng, Mengnan Li, Meng Kan, Yuxuan Li, Benke Xu, Ruiqi Wu, Yuanyuan Wang, Shengchao Zheng, Xiaoke Feng, Weisheng Molecules Article Corydalis humosa Migo is a traditional Chinese medicine that clears away damp heat, relieves sore. Protopine (PRO) is an alkaloid component isolated from C. humosa Migo. However, the role of protopine in acute kidney injury (AKI) has not yet been reported. This study aims to investigate the effect and mechanism of protopine isolated from C. humosa Migo on lipopolysaccharide (LPS)-induced AKI in mice. Inflammation accumulation was assessed by small animal living imaging. The blood urea nitrogen (BUN), and serum creatinine (Scr) were measured to assess the effects of protopine on renal function in LPS-induced AKI. The levels of tumor necrosis factor (TNF), interleukin-2 (IL-2), interferon-γ (IFN-γ), and (interleukin-10) IL-10 in serum were detected by cytometric bead array. Flow cytometry was used to detect the levels of reactive oxygen species (ROS) in primary kidney cells. The proportions of granulocytes, neutrophils, and macrophages in peripheral blood were examined to evaluate the effect of protopine on immune cells in mice with AKI. Toll-like receptor (TLR4) and apoptotic signaling pathway were detected by Western blot analysis. The results showed that protopine markedly improved the renal function, relieve inflammation, reversed inflammatory cytokines, transformed apoptosis markers, and regulated the TLR4 signaling pathway in mice with AKI induced by LPS. The protopine isolated from C. humosa Migo protected mice against LPS-induced AKI by inhibiting apoptosis and inflammation via the TLR4 signaling pathway, thus providing a molecular basis for a novel medical treatment of AKI. MDPI 2019-12-19 /pmc/articles/PMC6982873/ /pubmed/31861525 http://dx.doi.org/10.3390/molecules25010015 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Beibei
Zeng, Mengnan
Li, Meng
Kan, Yuxuan
Li, Benke
Xu, Ruiqi
Wu, Yuanyuan
Wang, Shengchao
Zheng, Xiaoke
Feng, Weisheng
Protopine Protects Mice against LPS-Induced Acute Kidney Injury by Inhibiting Apoptosis and Inflammation via the TLR4 Signaling Pathway
title Protopine Protects Mice against LPS-Induced Acute Kidney Injury by Inhibiting Apoptosis and Inflammation via the TLR4 Signaling Pathway
title_full Protopine Protects Mice against LPS-Induced Acute Kidney Injury by Inhibiting Apoptosis and Inflammation via the TLR4 Signaling Pathway
title_fullStr Protopine Protects Mice against LPS-Induced Acute Kidney Injury by Inhibiting Apoptosis and Inflammation via the TLR4 Signaling Pathway
title_full_unstemmed Protopine Protects Mice against LPS-Induced Acute Kidney Injury by Inhibiting Apoptosis and Inflammation via the TLR4 Signaling Pathway
title_short Protopine Protects Mice against LPS-Induced Acute Kidney Injury by Inhibiting Apoptosis and Inflammation via the TLR4 Signaling Pathway
title_sort protopine protects mice against lps-induced acute kidney injury by inhibiting apoptosis and inflammation via the tlr4 signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982873/
https://www.ncbi.nlm.nih.gov/pubmed/31861525
http://dx.doi.org/10.3390/molecules25010015
work_keys_str_mv AT zhangbeibei protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway
AT zengmengnan protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway
AT limeng protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway
AT kanyuxuan protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway
AT libenke protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway
AT xuruiqi protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway
AT wuyuanyuan protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway
AT wangshengchao protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway
AT zhengxiaoke protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway
AT fengweisheng protopineprotectsmiceagainstlpsinducedacutekidneyinjurybyinhibitingapoptosisandinflammationviathetlr4signalingpathway