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L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction

Lipid metabolism dysfunction and obesity are serious health issues to human beings. The current study investigated the effects of hyperbaric oxygen (HBO) against high fat diet (HFD)-induced lipid metabolism dysfunction and the roles of L-carnitine. C57/B6 mice were fed with HFD or normal chew diet,...

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Autores principales: Yuan, Junhua, Jiang, Qixiao, Song, Limin, Liu, Yuan, Li, Manwen, Lin, Qian, Li, Yanrun, Su, Kaizhen, Ma, Zhengye, Wang, Yifei, Liu, Defeng, Dong, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982999/
https://www.ncbi.nlm.nih.gov/pubmed/31906305
http://dx.doi.org/10.3390/molecules25010176
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author Yuan, Junhua
Jiang, Qixiao
Song, Limin
Liu, Yuan
Li, Manwen
Lin, Qian
Li, Yanrun
Su, Kaizhen
Ma, Zhengye
Wang, Yifei
Liu, Defeng
Dong, Jing
author_facet Yuan, Junhua
Jiang, Qixiao
Song, Limin
Liu, Yuan
Li, Manwen
Lin, Qian
Li, Yanrun
Su, Kaizhen
Ma, Zhengye
Wang, Yifei
Liu, Defeng
Dong, Jing
author_sort Yuan, Junhua
collection PubMed
description Lipid metabolism dysfunction and obesity are serious health issues to human beings. The current study investigated the effects of hyperbaric oxygen (HBO) against high fat diet (HFD)-induced lipid metabolism dysfunction and the roles of L-carnitine. C57/B6 mice were fed with HFD or normal chew diet, with or without HBO treatment. Histopathological methods were used to assess the adipose tissues, serum free fatty acid (FFA) levels were assessed with enzymatic methods, and the endogenous circulation and skeletal muscle L-carnitine levels were assessed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, western blotting was used to assess the expression levels of PPARα, CPT1b, pHSL/HSL, and UCP1. HFD treatment increased body/adipose tissue weight, serum FFA levels, circulation L-carnitines and decreased skeletal muscle L-carnitine levels, while HBO treatment alleviated such changes. Moreover, HFD treatment increased fatty acid deposition in adipose tissues and decreased the expression of HSL, while HBO treatment alleviated such changes. Additionally, HFD treatment decreased the expression levels of PPARα and increased those of CPT1b in skeletal muscle, while HBO treatment effectively reverted such changes as well. In brown adipose tissues, HFD increased the expression of UCP1 and the phosphorylation of HSL, which was abolished by HBO treatment as well. In summary, HBO treatment may alleviate HFD-induced fatty acid metabolism dysfunction in C57/B6 mice, which seems to be associated with circulation and skeletal muscle L-carnitine levels and PPARα expression.
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spelling pubmed-69829992020-02-06 L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction Yuan, Junhua Jiang, Qixiao Song, Limin Liu, Yuan Li, Manwen Lin, Qian Li, Yanrun Su, Kaizhen Ma, Zhengye Wang, Yifei Liu, Defeng Dong, Jing Molecules Article Lipid metabolism dysfunction and obesity are serious health issues to human beings. The current study investigated the effects of hyperbaric oxygen (HBO) against high fat diet (HFD)-induced lipid metabolism dysfunction and the roles of L-carnitine. C57/B6 mice were fed with HFD or normal chew diet, with or without HBO treatment. Histopathological methods were used to assess the adipose tissues, serum free fatty acid (FFA) levels were assessed with enzymatic methods, and the endogenous circulation and skeletal muscle L-carnitine levels were assessed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, western blotting was used to assess the expression levels of PPARα, CPT1b, pHSL/HSL, and UCP1. HFD treatment increased body/adipose tissue weight, serum FFA levels, circulation L-carnitines and decreased skeletal muscle L-carnitine levels, while HBO treatment alleviated such changes. Moreover, HFD treatment increased fatty acid deposition in adipose tissues and decreased the expression of HSL, while HBO treatment alleviated such changes. Additionally, HFD treatment decreased the expression levels of PPARα and increased those of CPT1b in skeletal muscle, while HBO treatment effectively reverted such changes as well. In brown adipose tissues, HFD increased the expression of UCP1 and the phosphorylation of HSL, which was abolished by HBO treatment as well. In summary, HBO treatment may alleviate HFD-induced fatty acid metabolism dysfunction in C57/B6 mice, which seems to be associated with circulation and skeletal muscle L-carnitine levels and PPARα expression. MDPI 2020-01-01 /pmc/articles/PMC6982999/ /pubmed/31906305 http://dx.doi.org/10.3390/molecules25010176 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yuan, Junhua
Jiang, Qixiao
Song, Limin
Liu, Yuan
Li, Manwen
Lin, Qian
Li, Yanrun
Su, Kaizhen
Ma, Zhengye
Wang, Yifei
Liu, Defeng
Dong, Jing
L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction
title L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction
title_full L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction
title_fullStr L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction
title_full_unstemmed L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction
title_short L-Carnitine Is Involved in Hyperbaric Oxygen-Mediated Therapeutic Effects in High Fat Diet-Induced Lipid Metabolism Dysfunction
title_sort l-carnitine is involved in hyperbaric oxygen-mediated therapeutic effects in high fat diet-induced lipid metabolism dysfunction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982999/
https://www.ncbi.nlm.nih.gov/pubmed/31906305
http://dx.doi.org/10.3390/molecules25010176
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