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Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus roxburghii (Wall.) Lindl by Response Surface Methodology (RSM)
The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant Anoectochilus roxburghii. Ultrasound assisted extraction (UAE) and Ultra-high performance liquid chromatography (UPLC) method were used to extract and determine the content of kinsenosid...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983077/ https://www.ncbi.nlm.nih.gov/pubmed/31906599 http://dx.doi.org/10.3390/molecules25010193 |
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author | Yang, Biyun Zhang, Mengyuan Weng, Haiyong Xu, Yong Zeng, Lihui |
author_facet | Yang, Biyun Zhang, Mengyuan Weng, Haiyong Xu, Yong Zeng, Lihui |
author_sort | Yang, Biyun |
collection | PubMed |
description | The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant Anoectochilus roxburghii. Ultrasound assisted extraction (UAE) and Ultra-high performance liquid chromatography (UPLC) method were used to extract and determine the content of kinsenoside, while response surface method (RSM) was used to optimize the extraction process. The best possible range for methanol concentration (0–100%), the liquid-solid ratio (5:1–30:1 mL/g), ultrasonic power (240–540 W), duration of ultrasound (10–50 min), ultrasonic temperature (10–60 °C), and the number of extractions (1–4) were obtained according to the single factor experiments. Then, using the Box-Behnken design (BBD) of response surface analysis, the optimum extraction conditions were obtained with 16.33% methanol concentration, the liquid-solid ratio of 10.83:1 mL/g and 35.00 °C ultrasonic temperature. Under these conditions, kinsenoside extraction yield reached 32.24% dry weight. The best conditions were applied to determine the kinsenoside content in seven different cultivation ages in Anoectochilus roxburghii. |
format | Online Article Text |
id | pubmed-6983077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69830772020-02-06 Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus roxburghii (Wall.) Lindl by Response Surface Methodology (RSM) Yang, Biyun Zhang, Mengyuan Weng, Haiyong Xu, Yong Zeng, Lihui Molecules Article The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant Anoectochilus roxburghii. Ultrasound assisted extraction (UAE) and Ultra-high performance liquid chromatography (UPLC) method were used to extract and determine the content of kinsenoside, while response surface method (RSM) was used to optimize the extraction process. The best possible range for methanol concentration (0–100%), the liquid-solid ratio (5:1–30:1 mL/g), ultrasonic power (240–540 W), duration of ultrasound (10–50 min), ultrasonic temperature (10–60 °C), and the number of extractions (1–4) were obtained according to the single factor experiments. Then, using the Box-Behnken design (BBD) of response surface analysis, the optimum extraction conditions were obtained with 16.33% methanol concentration, the liquid-solid ratio of 10.83:1 mL/g and 35.00 °C ultrasonic temperature. Under these conditions, kinsenoside extraction yield reached 32.24% dry weight. The best conditions were applied to determine the kinsenoside content in seven different cultivation ages in Anoectochilus roxburghii. MDPI 2020-01-02 /pmc/articles/PMC6983077/ /pubmed/31906599 http://dx.doi.org/10.3390/molecules25010193 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Biyun Zhang, Mengyuan Weng, Haiyong Xu, Yong Zeng, Lihui Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus roxburghii (Wall.) Lindl by Response Surface Methodology (RSM) |
title | Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus
roxburghii (Wall.) Lindl by Response Surface Methodology (RSM) |
title_full | Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus
roxburghii (Wall.) Lindl by Response Surface Methodology (RSM) |
title_fullStr | Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus
roxburghii (Wall.) Lindl by Response Surface Methodology (RSM) |
title_full_unstemmed | Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus
roxburghii (Wall.) Lindl by Response Surface Methodology (RSM) |
title_short | Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus
roxburghii (Wall.) Lindl by Response Surface Methodology (RSM) |
title_sort | optimization of ultrasound assisted extraction (uae) of kinsenoside compound from anoectochilus
roxburghii (wall.) lindl by response surface methodology (rsm) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983077/ https://www.ncbi.nlm.nih.gov/pubmed/31906599 http://dx.doi.org/10.3390/molecules25010193 |
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