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Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity

To gain a better understanding of how nitrate may affect carbohydrate and lipid metabolism, female wild-type mice were fed a high-fat, high-fructose diet supplemented with either 0, 400, or 800 mg nitrate/kg diet for 28 days. Additionally, obese female db/db mice were fed a 5% fat diet supplemented...

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Autores principales: Fischer, Alexandra, Lüersen, Kai, Schultheiß, Gerhard, de Pascual-Teresa, Sonia, Mereu, Alessandro, Ipharraguerre, Ignacio R., Rimbach, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983433/
https://www.ncbi.nlm.nih.gov/pubmed/32001953
http://dx.doi.org/10.3164/jcbn.19-43
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author Fischer, Alexandra
Lüersen, Kai
Schultheiß, Gerhard
de Pascual-Teresa, Sonia
Mereu, Alessandro
Ipharraguerre, Ignacio R.
Rimbach, Gerald
author_facet Fischer, Alexandra
Lüersen, Kai
Schultheiß, Gerhard
de Pascual-Teresa, Sonia
Mereu, Alessandro
Ipharraguerre, Ignacio R.
Rimbach, Gerald
author_sort Fischer, Alexandra
collection PubMed
description To gain a better understanding of how nitrate may affect carbohydrate and lipid metabolism, female wild-type mice were fed a high-fat, high-fructose diet supplemented with either 0, 400, or 800 mg nitrate/kg diet for 28 days. Additionally, obese female db/db mice were fed a 5% fat diet supplemented with the same levels and source of nitrate. Nitrate decreased the sodium-dependent uptake of glucose by ileal mucosa in wild-type mice. Moreover, nitrate significantly decreased triglyceride content and mRNA expression levels of Pparγ in liver and Glut4 in skeletal muscle. Oral glucose tolerance as well as plasma cholesterol, triglyceride, insulin, leptin, glucose and the activity of ALT did not significantly differ between experimental groups but was higher in db/db mice than in wild-type mice. Nitrate changed liver fatty acid composition and mRNA levels of Fads only slightly. Further hepatic genes encoding proteins involved in lipid and carbohydrate metabolism were not significantly different between the three groups. Biomarkers of inflammation and autophagy in the liver were not affected by the different dietary treatments. Overall, the present data suggest that short-term dietary supplementation with inorganic nitrate has only modest effects on carbohydrate and lipid metabolism in genetic and dietary-induced mouse models of obesity.
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spelling pubmed-69834332020-01-30 Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity Fischer, Alexandra Lüersen, Kai Schultheiß, Gerhard de Pascual-Teresa, Sonia Mereu, Alessandro Ipharraguerre, Ignacio R. Rimbach, Gerald J Clin Biochem Nutr Original Article To gain a better understanding of how nitrate may affect carbohydrate and lipid metabolism, female wild-type mice were fed a high-fat, high-fructose diet supplemented with either 0, 400, or 800 mg nitrate/kg diet for 28 days. Additionally, obese female db/db mice were fed a 5% fat diet supplemented with the same levels and source of nitrate. Nitrate decreased the sodium-dependent uptake of glucose by ileal mucosa in wild-type mice. Moreover, nitrate significantly decreased triglyceride content and mRNA expression levels of Pparγ in liver and Glut4 in skeletal muscle. Oral glucose tolerance as well as plasma cholesterol, triglyceride, insulin, leptin, glucose and the activity of ALT did not significantly differ between experimental groups but was higher in db/db mice than in wild-type mice. Nitrate changed liver fatty acid composition and mRNA levels of Fads only slightly. Further hepatic genes encoding proteins involved in lipid and carbohydrate metabolism were not significantly different between the three groups. Biomarkers of inflammation and autophagy in the liver were not affected by the different dietary treatments. Overall, the present data suggest that short-term dietary supplementation with inorganic nitrate has only modest effects on carbohydrate and lipid metabolism in genetic and dietary-induced mouse models of obesity. the Society for Free Radical Research Japan 2020-01 2019-11-12 /pmc/articles/PMC6983433/ /pubmed/32001953 http://dx.doi.org/10.3164/jcbn.19-43 Text en Copyright © 2020 JCBN http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Fischer, Alexandra
Lüersen, Kai
Schultheiß, Gerhard
de Pascual-Teresa, Sonia
Mereu, Alessandro
Ipharraguerre, Ignacio R.
Rimbach, Gerald
Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity
title Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity
title_full Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity
title_fullStr Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity
title_full_unstemmed Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity
title_short Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity
title_sort supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983433/
https://www.ncbi.nlm.nih.gov/pubmed/32001953
http://dx.doi.org/10.3164/jcbn.19-43
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