Cargando…

High-speed simultaneous multiscale photoacoustic microscopy

Photoacoustic microscopy (PAM) is a fast-growing biomedical imaging technique that provides high-resolution in vivo imaging beyond the optical diffusion limit. Depending on the scalable lateral resolution and achievable penetration depth, PAM can be classified into optical resolution PAM (OR-PAM) an...

Descripción completa

Detalles Bibliográficos
Autores principales: Moothanchery, Mohesh, Bi, Renzhe, Kim, Jin Young, Balasundaram, Ghayathri, Kim, Chulhong, Olivo, Malini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983484/
https://www.ncbi.nlm.nih.gov/pubmed/31429217
http://dx.doi.org/10.1117/1.JBO.24.8.086001
_version_ 1783491506772901888
author Moothanchery, Mohesh
Bi, Renzhe
Kim, Jin Young
Balasundaram, Ghayathri
Kim, Chulhong
Olivo, Malini
author_facet Moothanchery, Mohesh
Bi, Renzhe
Kim, Jin Young
Balasundaram, Ghayathri
Kim, Chulhong
Olivo, Malini
author_sort Moothanchery, Mohesh
collection PubMed
description Photoacoustic microscopy (PAM) is a fast-growing biomedical imaging technique that provides high-resolution in vivo imaging beyond the optical diffusion limit. Depending on the scalable lateral resolution and achievable penetration depth, PAM can be classified into optical resolution PAM (OR-PAM) and acoustic resolution PAM (AR-PAM). The use of a microelectromechanical systems (MEMS) scanner has improved OR-PAM imaging speed significantly and is highly beneficial in the development of miniaturized handheld devices. The shallow penetration depth of OR-PAM limits the use of such devices for a wide range of clinical applications. We report the use of a high-speed MEMS scanner for both OR-PAM and AR-PAM. A high-speed, wide-area scanning integrated OR-AR-PAM system combining MEMS scanner and raster mechanical movement was developed. A lateral resolution of [Formula: see text] and penetration depth [Formula: see text] in vivo was achieved using OR-PAM at 586 nm, whereas a lateral resolution of [Formula: see text] and penetration depth of [Formula: see text] in vivo was achieved using AR-PAM at 532 nm.
format Online
Article
Text
id pubmed-6983484
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Society of Photo-Optical Instrumentation Engineers
record_format MEDLINE/PubMed
spelling pubmed-69834842020-02-03 High-speed simultaneous multiscale photoacoustic microscopy Moothanchery, Mohesh Bi, Renzhe Kim, Jin Young Balasundaram, Ghayathri Kim, Chulhong Olivo, Malini J Biomed Opt Imaging Photoacoustic microscopy (PAM) is a fast-growing biomedical imaging technique that provides high-resolution in vivo imaging beyond the optical diffusion limit. Depending on the scalable lateral resolution and achievable penetration depth, PAM can be classified into optical resolution PAM (OR-PAM) and acoustic resolution PAM (AR-PAM). The use of a microelectromechanical systems (MEMS) scanner has improved OR-PAM imaging speed significantly and is highly beneficial in the development of miniaturized handheld devices. The shallow penetration depth of OR-PAM limits the use of such devices for a wide range of clinical applications. We report the use of a high-speed MEMS scanner for both OR-PAM and AR-PAM. A high-speed, wide-area scanning integrated OR-AR-PAM system combining MEMS scanner and raster mechanical movement was developed. A lateral resolution of [Formula: see text] and penetration depth [Formula: see text] in vivo was achieved using OR-PAM at 586 nm, whereas a lateral resolution of [Formula: see text] and penetration depth of [Formula: see text] in vivo was achieved using AR-PAM at 532 nm. Society of Photo-Optical Instrumentation Engineers 2019-08-19 2019-08 /pmc/articles/PMC6983484/ /pubmed/31429217 http://dx.doi.org/10.1117/1.JBO.24.8.086001 Text en © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle Imaging
Moothanchery, Mohesh
Bi, Renzhe
Kim, Jin Young
Balasundaram, Ghayathri
Kim, Chulhong
Olivo, Malini
High-speed simultaneous multiscale photoacoustic microscopy
title High-speed simultaneous multiscale photoacoustic microscopy
title_full High-speed simultaneous multiscale photoacoustic microscopy
title_fullStr High-speed simultaneous multiscale photoacoustic microscopy
title_full_unstemmed High-speed simultaneous multiscale photoacoustic microscopy
title_short High-speed simultaneous multiscale photoacoustic microscopy
title_sort high-speed simultaneous multiscale photoacoustic microscopy
topic Imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983484/
https://www.ncbi.nlm.nih.gov/pubmed/31429217
http://dx.doi.org/10.1117/1.JBO.24.8.086001
work_keys_str_mv AT moothancherymohesh highspeedsimultaneousmultiscalephotoacousticmicroscopy
AT birenzhe highspeedsimultaneousmultiscalephotoacousticmicroscopy
AT kimjinyoung highspeedsimultaneousmultiscalephotoacousticmicroscopy
AT balasundaramghayathri highspeedsimultaneousmultiscalephotoacousticmicroscopy
AT kimchulhong highspeedsimultaneousmultiscalephotoacousticmicroscopy
AT olivomalini highspeedsimultaneousmultiscalephotoacousticmicroscopy