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High-speed simultaneous multiscale photoacoustic microscopy
Photoacoustic microscopy (PAM) is a fast-growing biomedical imaging technique that provides high-resolution in vivo imaging beyond the optical diffusion limit. Depending on the scalable lateral resolution and achievable penetration depth, PAM can be classified into optical resolution PAM (OR-PAM) an...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983484/ https://www.ncbi.nlm.nih.gov/pubmed/31429217 http://dx.doi.org/10.1117/1.JBO.24.8.086001 |
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author | Moothanchery, Mohesh Bi, Renzhe Kim, Jin Young Balasundaram, Ghayathri Kim, Chulhong Olivo, Malini |
author_facet | Moothanchery, Mohesh Bi, Renzhe Kim, Jin Young Balasundaram, Ghayathri Kim, Chulhong Olivo, Malini |
author_sort | Moothanchery, Mohesh |
collection | PubMed |
description | Photoacoustic microscopy (PAM) is a fast-growing biomedical imaging technique that provides high-resolution in vivo imaging beyond the optical diffusion limit. Depending on the scalable lateral resolution and achievable penetration depth, PAM can be classified into optical resolution PAM (OR-PAM) and acoustic resolution PAM (AR-PAM). The use of a microelectromechanical systems (MEMS) scanner has improved OR-PAM imaging speed significantly and is highly beneficial in the development of miniaturized handheld devices. The shallow penetration depth of OR-PAM limits the use of such devices for a wide range of clinical applications. We report the use of a high-speed MEMS scanner for both OR-PAM and AR-PAM. A high-speed, wide-area scanning integrated OR-AR-PAM system combining MEMS scanner and raster mechanical movement was developed. A lateral resolution of [Formula: see text] and penetration depth [Formula: see text] in vivo was achieved using OR-PAM at 586 nm, whereas a lateral resolution of [Formula: see text] and penetration depth of [Formula: see text] in vivo was achieved using AR-PAM at 532 nm. |
format | Online Article Text |
id | pubmed-6983484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-69834842020-02-03 High-speed simultaneous multiscale photoacoustic microscopy Moothanchery, Mohesh Bi, Renzhe Kim, Jin Young Balasundaram, Ghayathri Kim, Chulhong Olivo, Malini J Biomed Opt Imaging Photoacoustic microscopy (PAM) is a fast-growing biomedical imaging technique that provides high-resolution in vivo imaging beyond the optical diffusion limit. Depending on the scalable lateral resolution and achievable penetration depth, PAM can be classified into optical resolution PAM (OR-PAM) and acoustic resolution PAM (AR-PAM). The use of a microelectromechanical systems (MEMS) scanner has improved OR-PAM imaging speed significantly and is highly beneficial in the development of miniaturized handheld devices. The shallow penetration depth of OR-PAM limits the use of such devices for a wide range of clinical applications. We report the use of a high-speed MEMS scanner for both OR-PAM and AR-PAM. A high-speed, wide-area scanning integrated OR-AR-PAM system combining MEMS scanner and raster mechanical movement was developed. A lateral resolution of [Formula: see text] and penetration depth [Formula: see text] in vivo was achieved using OR-PAM at 586 nm, whereas a lateral resolution of [Formula: see text] and penetration depth of [Formula: see text] in vivo was achieved using AR-PAM at 532 nm. Society of Photo-Optical Instrumentation Engineers 2019-08-19 2019-08 /pmc/articles/PMC6983484/ /pubmed/31429217 http://dx.doi.org/10.1117/1.JBO.24.8.086001 Text en © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | Imaging Moothanchery, Mohesh Bi, Renzhe Kim, Jin Young Balasundaram, Ghayathri Kim, Chulhong Olivo, Malini High-speed simultaneous multiscale photoacoustic microscopy |
title | High-speed simultaneous multiscale photoacoustic microscopy |
title_full | High-speed simultaneous multiscale photoacoustic microscopy |
title_fullStr | High-speed simultaneous multiscale photoacoustic microscopy |
title_full_unstemmed | High-speed simultaneous multiscale photoacoustic microscopy |
title_short | High-speed simultaneous multiscale photoacoustic microscopy |
title_sort | high-speed simultaneous multiscale photoacoustic microscopy |
topic | Imaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983484/ https://www.ncbi.nlm.nih.gov/pubmed/31429217 http://dx.doi.org/10.1117/1.JBO.24.8.086001 |
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