Cargando…
Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation
Instrument cost is a major problem for the transduction of DNA fragments and proteins into cells. Water-in-oil droplet electroporation (droplet-EP) was recently invented as a low-cost and effective method for the transfection of plasmids into cultured human cells. We here applied droplet-EP to lives...
Autores principales: | ISHINO, Takeshi, KURITA, Hirofumi, KIRISAWA, Rikio, SHIMAMOTO, Yoshinori, NUMANO, Rika, KITAMURA, Hiroshi |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983666/ https://www.ncbi.nlm.nih.gov/pubmed/31776296 http://dx.doi.org/10.1292/jvms.19-0475 |
Ejemplares similares
-
Novel Parallelized Electroporation by Electrostatic Manipulation of a Water-in-Oil Droplet as a Microreactor
por: Kurita, Hirofumi, et al.
Publicado: (2015) -
Influence of Electroporation Medium on Delivery of Cell-Impermeable Small Molecules by Electrical Short-Circuiting via an Aqueous Droplet in Dielectric Oil: A Comparison of Different Fluorescent Tracers
por: Watanabe, Yuki, et al.
Publicado: (2022) -
Influence of DNA characteristics on cell membrane damage stimulated by electrical short-circuiting via a low-conductive aqueous droplet in dielectric oil
por: Tsurusaki, Yoshino, et al.
Publicado: (2023) -
Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field
por: Kurita, Hirofumi, et al.
Publicado: (2016) -
Mechanistic studies of gene delivery into mammalian cells by electrical short-circuiting via an aqueous droplet in dielectric oil
por: Kurita, Hirofumi, et al.
Publicado: (2020)