Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene

We performed a clonality analysis using polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) gene rearrangement, specifically with regard to its utility as a method to diagnose bovine B-cell lymphoma. PCR for IgH gene rearrangement indicated monoclonal proliferation of B-cells in 24...

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Autores principales: MAEZAWA, Masaki, WATANABE, Ken-ichi, HORIUCHI, Noriyuki, MATSUMOTO, Kotaro, KOBAYASHI, Yoshiyasu, INOKUMA, Hisashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2019
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983668/
https://www.ncbi.nlm.nih.gov/pubmed/31801928
http://dx.doi.org/10.1292/jvms.19-0418
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author MAEZAWA, Masaki
WATANABE, Ken-ichi
HORIUCHI, Noriyuki
MATSUMOTO, Kotaro
KOBAYASHI, Yoshiyasu
INOKUMA, Hisashi
author_facet MAEZAWA, Masaki
WATANABE, Ken-ichi
HORIUCHI, Noriyuki
MATSUMOTO, Kotaro
KOBAYASHI, Yoshiyasu
INOKUMA, Hisashi
author_sort MAEZAWA, Masaki
collection PubMed
description We performed a clonality analysis using polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) gene rearrangement, specifically with regard to its utility as a method to diagnose bovine B-cell lymphoma. PCR for IgH gene rearrangement indicated monoclonal proliferation of B-cells in 24 of 35 cattle with B-cell lymphoma. In contrast, PCR for IgH gene rearrangement in lymph nodes and tumor tissues from 65 cattle diagnosed with tumors other than B-cell lymphoma and non-tumors revealed polyclonal population of B-cells. Sensitivity, specificity, positive predictive value, and negative predictive value for PCR for IgH gene rearrangement for bovine B-cell lymphoma were 68.6%, 100%, 100%, and 85.5%, respectively. Clonality analysis using PCR for IgH gene rearrangement may be useful for adjunctive diagnosis of bovine B-cell lymphoma.
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spelling pubmed-69836682020-01-30 Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene MAEZAWA, Masaki WATANABE, Ken-ichi HORIUCHI, Noriyuki MATSUMOTO, Kotaro KOBAYASHI, Yoshiyasu INOKUMA, Hisashi J Vet Med Sci Internal Medicine We performed a clonality analysis using polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) gene rearrangement, specifically with regard to its utility as a method to diagnose bovine B-cell lymphoma. PCR for IgH gene rearrangement indicated monoclonal proliferation of B-cells in 24 of 35 cattle with B-cell lymphoma. In contrast, PCR for IgH gene rearrangement in lymph nodes and tumor tissues from 65 cattle diagnosed with tumors other than B-cell lymphoma and non-tumors revealed polyclonal population of B-cells. Sensitivity, specificity, positive predictive value, and negative predictive value for PCR for IgH gene rearrangement for bovine B-cell lymphoma were 68.6%, 100%, 100%, and 85.5%, respectively. Clonality analysis using PCR for IgH gene rearrangement may be useful for adjunctive diagnosis of bovine B-cell lymphoma. The Japanese Society of Veterinary Science 2019-12-04 2020-01 /pmc/articles/PMC6983668/ /pubmed/31801928 http://dx.doi.org/10.1292/jvms.19-0418 Text en ©2020 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Internal Medicine
MAEZAWA, Masaki
WATANABE, Ken-ichi
HORIUCHI, Noriyuki
MATSUMOTO, Kotaro
KOBAYASHI, Yoshiyasu
INOKUMA, Hisashi
Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene
title Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene
title_full Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene
title_fullStr Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene
title_full_unstemmed Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene
title_short Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene
title_sort molecular diagnosis of bovine b-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983668/
https://www.ncbi.nlm.nih.gov/pubmed/31801928
http://dx.doi.org/10.1292/jvms.19-0418
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