Cargando…
Molecular cloning of Gibel carp (Carassius auratus gibelio) complement component C3 and its expression profile after Cyprinid herpesvirus 2 infection
The complement systems play an important role in innate and adaptive immunity. In this study, the complement C3 gene, designated CagC3, was cloned and sequenced from Gibel carp (Carassius auratus gibelio). The expression pattern of CagC3 in different tissues of healthy Gibel carp and after challenge...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983669/ https://www.ncbi.nlm.nih.gov/pubmed/31723065 http://dx.doi.org/10.1292/jvms.18-0125 |
_version_ | 1783491545629982720 |
---|---|
author | FAN, Yuding ZHANG, Xueping ZHOU, Yong JIANG, Nan LIU, Wenzhi ZENG, Lingbing |
author_facet | FAN, Yuding ZHANG, Xueping ZHOU, Yong JIANG, Nan LIU, Wenzhi ZENG, Lingbing |
author_sort | FAN, Yuding |
collection | PubMed |
description | The complement systems play an important role in innate and adaptive immunity. In this study, the complement C3 gene, designated CagC3, was cloned and sequenced from Gibel carp (Carassius auratus gibelio). The expression pattern of CagC3 in different tissues of healthy Gibel carp and after challenge with Cyprinid herpesvirus 2 (CyHV-2) were evaluated using quantitative real-time PCR. The full-length CagC3 cDNA was 5131 bp with an ORF of 4950 bp, encoding a predicted protein of 1649 amino acids. The deduced amino acid sequence showed that CagC3 has conserved domains and residues known to be critical for C3 function. Phylogenetic analysis demonstrated that CagC3 clustered with homologs from common carp and grass carp (Ctenopharyngodon idella). CagC3 is expressed in all examined tissues of healthy Gibel carp, with the highest expression in liver. In vivo, after CyHV-2 challenge, CagC3 transcription was significantly upregulated in liver, spleen and kidney with the peaks at 24 hr, 2 d, and 2 d, respectively. In vitro, CagC3 expression in the Gibel carp brain cell line showed the same pattern as that in vivo after stimulation with CyHV-2 or poly(I:C). However, CagC3 expression was downregulated at 24 hr after induction with lipopolysaccharide (LPS), and then reached the peak at 2 d. These results suggest that CagC3 is involved in the innate immune response of Gibel carp to viral infection. |
format | Online Article Text |
id | pubmed-6983669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-69836692020-01-30 Molecular cloning of Gibel carp (Carassius auratus gibelio) complement component C3 and its expression profile after Cyprinid herpesvirus 2 infection FAN, Yuding ZHANG, Xueping ZHOU, Yong JIANG, Nan LIU, Wenzhi ZENG, Lingbing J Vet Med Sci Immunology The complement systems play an important role in innate and adaptive immunity. In this study, the complement C3 gene, designated CagC3, was cloned and sequenced from Gibel carp (Carassius auratus gibelio). The expression pattern of CagC3 in different tissues of healthy Gibel carp and after challenge with Cyprinid herpesvirus 2 (CyHV-2) were evaluated using quantitative real-time PCR. The full-length CagC3 cDNA was 5131 bp with an ORF of 4950 bp, encoding a predicted protein of 1649 amino acids. The deduced amino acid sequence showed that CagC3 has conserved domains and residues known to be critical for C3 function. Phylogenetic analysis demonstrated that CagC3 clustered with homologs from common carp and grass carp (Ctenopharyngodon idella). CagC3 is expressed in all examined tissues of healthy Gibel carp, with the highest expression in liver. In vivo, after CyHV-2 challenge, CagC3 transcription was significantly upregulated in liver, spleen and kidney with the peaks at 24 hr, 2 d, and 2 d, respectively. In vitro, CagC3 expression in the Gibel carp brain cell line showed the same pattern as that in vivo after stimulation with CyHV-2 or poly(I:C). However, CagC3 expression was downregulated at 24 hr after induction with lipopolysaccharide (LPS), and then reached the peak at 2 d. These results suggest that CagC3 is involved in the innate immune response of Gibel carp to viral infection. The Japanese Society of Veterinary Science 2019-11-14 2020-01 /pmc/articles/PMC6983669/ /pubmed/31723065 http://dx.doi.org/10.1292/jvms.18-0125 Text en ©2020 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Immunology FAN, Yuding ZHANG, Xueping ZHOU, Yong JIANG, Nan LIU, Wenzhi ZENG, Lingbing Molecular cloning of Gibel carp (Carassius auratus gibelio) complement component C3 and its expression profile after Cyprinid herpesvirus 2 infection |
title | Molecular cloning of Gibel carp (Carassius auratus gibelio) complement component C3 and its expression profile after Cyprinid herpesvirus 2
infection |
title_full | Molecular cloning of Gibel carp (Carassius auratus gibelio) complement component C3 and its expression profile after Cyprinid herpesvirus 2
infection |
title_fullStr | Molecular cloning of Gibel carp (Carassius auratus gibelio) complement component C3 and its expression profile after Cyprinid herpesvirus 2
infection |
title_full_unstemmed | Molecular cloning of Gibel carp (Carassius auratus gibelio) complement component C3 and its expression profile after Cyprinid herpesvirus 2
infection |
title_short | Molecular cloning of Gibel carp (Carassius auratus gibelio) complement component C3 and its expression profile after Cyprinid herpesvirus 2
infection |
title_sort | molecular cloning of gibel carp (carassius auratus gibelio) complement component c3 and its expression profile after cyprinid herpesvirus 2
infection |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983669/ https://www.ncbi.nlm.nih.gov/pubmed/31723065 http://dx.doi.org/10.1292/jvms.18-0125 |
work_keys_str_mv | AT fanyuding molecularcloningofgibelcarpcarassiusauratusgibeliocomplementcomponentc3anditsexpressionprofileaftercyprinidherpesvirus2infection AT zhangxueping molecularcloningofgibelcarpcarassiusauratusgibeliocomplementcomponentc3anditsexpressionprofileaftercyprinidherpesvirus2infection AT zhouyong molecularcloningofgibelcarpcarassiusauratusgibeliocomplementcomponentc3anditsexpressionprofileaftercyprinidherpesvirus2infection AT jiangnan molecularcloningofgibelcarpcarassiusauratusgibeliocomplementcomponentc3anditsexpressionprofileaftercyprinidherpesvirus2infection AT liuwenzhi molecularcloningofgibelcarpcarassiusauratusgibeliocomplementcomponentc3anditsexpressionprofileaftercyprinidherpesvirus2infection AT zenglingbing molecularcloningofgibelcarpcarassiusauratusgibeliocomplementcomponentc3anditsexpressionprofileaftercyprinidherpesvirus2infection |