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Electrical impedance as an indicator of microalgal cell health

Separating specific cell phenotypes from a heterotypic mixture is a critical step in many research projects. Traditional methods usually require a large sample volume and a complex preparation process that may alter cell property during the sorting process. Here we present the use of electrical impe...

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Autores principales: Sui, Jianye, Foflonker, Fatima, Bhattacharya, Debashish, Javanmard, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985174/
https://www.ncbi.nlm.nih.gov/pubmed/31988339
http://dx.doi.org/10.1038/s41598-020-57541-6
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author Sui, Jianye
Foflonker, Fatima
Bhattacharya, Debashish
Javanmard, Mehdi
author_facet Sui, Jianye
Foflonker, Fatima
Bhattacharya, Debashish
Javanmard, Mehdi
author_sort Sui, Jianye
collection PubMed
description Separating specific cell phenotypes from a heterotypic mixture is a critical step in many research projects. Traditional methods usually require a large sample volume and a complex preparation process that may alter cell property during the sorting process. Here we present the use of electrical impedance as an indicator of cell health and for identifying specific microalgal phenotypes. We developed a microfluidic platform for measuring electrical impedance at different frequencies using the bacterium-sized green alga Picochlorum SE3. The cells were cultured under different salinity conditions and sampled at four different time points. Our results demonstrate the utility of electrical impedance as an indicator of cell phenotype by providing results that are consistent with known changes in cell size and physiology. Outliers in the cell data distribution are particularly useful because they represent phenotypes that have the ability to maintain size and/or membrane ionic permeability under prolonged salt stress. This suggests that our device can be used to identify and sort desired (e.g., experimentally evolved, mutant) cell phenotypes based on their electrical impedance properties.
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spelling pubmed-69851742020-01-31 Electrical impedance as an indicator of microalgal cell health Sui, Jianye Foflonker, Fatima Bhattacharya, Debashish Javanmard, Mehdi Sci Rep Article Separating specific cell phenotypes from a heterotypic mixture is a critical step in many research projects. Traditional methods usually require a large sample volume and a complex preparation process that may alter cell property during the sorting process. Here we present the use of electrical impedance as an indicator of cell health and for identifying specific microalgal phenotypes. We developed a microfluidic platform for measuring electrical impedance at different frequencies using the bacterium-sized green alga Picochlorum SE3. The cells were cultured under different salinity conditions and sampled at four different time points. Our results demonstrate the utility of electrical impedance as an indicator of cell phenotype by providing results that are consistent with known changes in cell size and physiology. Outliers in the cell data distribution are particularly useful because they represent phenotypes that have the ability to maintain size and/or membrane ionic permeability under prolonged salt stress. This suggests that our device can be used to identify and sort desired (e.g., experimentally evolved, mutant) cell phenotypes based on their electrical impedance properties. Nature Publishing Group UK 2020-01-27 /pmc/articles/PMC6985174/ /pubmed/31988339 http://dx.doi.org/10.1038/s41598-020-57541-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sui, Jianye
Foflonker, Fatima
Bhattacharya, Debashish
Javanmard, Mehdi
Electrical impedance as an indicator of microalgal cell health
title Electrical impedance as an indicator of microalgal cell health
title_full Electrical impedance as an indicator of microalgal cell health
title_fullStr Electrical impedance as an indicator of microalgal cell health
title_full_unstemmed Electrical impedance as an indicator of microalgal cell health
title_short Electrical impedance as an indicator of microalgal cell health
title_sort electrical impedance as an indicator of microalgal cell health
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985174/
https://www.ncbi.nlm.nih.gov/pubmed/31988339
http://dx.doi.org/10.1038/s41598-020-57541-6
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