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Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats

In this study, we sought to investigate the impact of photobiomodulation and adipose-derived stem cells (ADS), alone and in combination, on the maturation step of wound healing in an ischemic infected delayed healing wound model in rats with type 2 diabetes mellitus (DM2). We randomly divided 24 adu...

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Autores principales: Moradi, Ali, Zare, Fatemeh, Mostafavinia, Atarodsadat, Safaju, Sobhan, Shahbazi, Amirhossein, Habibi, Malihe, Abdollahifar, Mohammad-Amin, Hashemi, Seyed Mahmoud, Amini, Abdollah, Ghoreishi, Seyed Kamran, Chien, Sufan, Hamblin, Michael R., Kouhkheil, Reza, Bayat, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985227/
https://www.ncbi.nlm.nih.gov/pubmed/31988386
http://dx.doi.org/10.1038/s41598-020-58099-z
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author Moradi, Ali
Zare, Fatemeh
Mostafavinia, Atarodsadat
Safaju, Sobhan
Shahbazi, Amirhossein
Habibi, Malihe
Abdollahifar, Mohammad-Amin
Hashemi, Seyed Mahmoud
Amini, Abdollah
Ghoreishi, Seyed Kamran
Chien, Sufan
Hamblin, Michael R.
Kouhkheil, Reza
Bayat, Mohammad
author_facet Moradi, Ali
Zare, Fatemeh
Mostafavinia, Atarodsadat
Safaju, Sobhan
Shahbazi, Amirhossein
Habibi, Malihe
Abdollahifar, Mohammad-Amin
Hashemi, Seyed Mahmoud
Amini, Abdollah
Ghoreishi, Seyed Kamran
Chien, Sufan
Hamblin, Michael R.
Kouhkheil, Reza
Bayat, Mohammad
author_sort Moradi, Ali
collection PubMed
description In this study, we sought to investigate the impact of photobiomodulation and adipose-derived stem cells (ADS), alone and in combination, on the maturation step of wound healing in an ischemic infected delayed healing wound model in rats with type 2 diabetes mellitus (DM2). We randomly divided 24 adult male rats into 4 groups (n = 6 per group). DM2 plus an ischemic delayed healing wound were induced in all rats. The wounds were infected with methicillin-resistant Staphylococcus aureus. Group 1 was the control (placebo) group. Group 2 received only photobiomodulation (890 nm, 80 Hz, 0.324 J/cm(2), and 0.001 W/cm(2)). Group 3 received only the allograft ADS. Group 4 received allograft ADS followed by photobiomodulation. On days 0, 4, 8, 12, and 16, we performed microbiological examination (colony forming units, [CFU]), wound area measurement, wound closure rate, wound strength, and histological and stereological examinations. The results indicated that at day 16, there was significantly decreased CFU (Analysis of variance, p = 0.001) in the photobiomodulation + ADS (0.0 ± 0.0), ADS (1350 ± 212), and photobiomodulation (0.0 ± 0.0) groups compared with the control group (27250 ± 1284). There was significantly decreased wound area (Analysis of variance, p = 0.000) in the photobiomodulation + ADS (7.4 ± 1.4 mm(2)), ADS (11 ± 2.2 mm(2)), and photobiomodulation (11.4 ± 1.4 mm(2)) groups compared with the control group (25.2 ± 1.7). There was a significantly increased tensiometeric property (stress maximal load, Analysis of variance, p = 0.000) in the photobiomodulation + ADS (0.99 ± 0.06 N/cm(2)), ADS (0.51 ± 0.12 N/cm(2)), and photobiomodulation (0.35 ± 0.15 N/cm(2)) groups compared with the control group (0.18 ± 0.04). There was a significantly modulated inflammatory response in (Analysis of variance, p = 0.049) in the photobiomodulation + ADS (337 ± 96), ADS (1175 ± 640), and photobiomodulation (69 ± 54) treatments compared to control group (7321 ± 4099). Photobiomodulation + ADS gave significantly better improvements in CFU, wound area, and wound strength compared to photobiomodulation or ADS alone. Photobiomodulation, ADS, and their combination significantly hastened healing in ischemic methicillin-resistant Staphylococcus aureus infected delayed healing wounds in rats with DM2. Combined application of photobiomodulation plus ADS demonstrated an additive effect.
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spelling pubmed-69852272020-01-31 Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats Moradi, Ali Zare, Fatemeh Mostafavinia, Atarodsadat Safaju, Sobhan Shahbazi, Amirhossein Habibi, Malihe Abdollahifar, Mohammad-Amin Hashemi, Seyed Mahmoud Amini, Abdollah Ghoreishi, Seyed Kamran Chien, Sufan Hamblin, Michael R. Kouhkheil, Reza Bayat, Mohammad Sci Rep Article In this study, we sought to investigate the impact of photobiomodulation and adipose-derived stem cells (ADS), alone and in combination, on the maturation step of wound healing in an ischemic infected delayed healing wound model in rats with type 2 diabetes mellitus (DM2). We randomly divided 24 adult male rats into 4 groups (n = 6 per group). DM2 plus an ischemic delayed healing wound were induced in all rats. The wounds were infected with methicillin-resistant Staphylococcus aureus. Group 1 was the control (placebo) group. Group 2 received only photobiomodulation (890 nm, 80 Hz, 0.324 J/cm(2), and 0.001 W/cm(2)). Group 3 received only the allograft ADS. Group 4 received allograft ADS followed by photobiomodulation. On days 0, 4, 8, 12, and 16, we performed microbiological examination (colony forming units, [CFU]), wound area measurement, wound closure rate, wound strength, and histological and stereological examinations. The results indicated that at day 16, there was significantly decreased CFU (Analysis of variance, p = 0.001) in the photobiomodulation + ADS (0.0 ± 0.0), ADS (1350 ± 212), and photobiomodulation (0.0 ± 0.0) groups compared with the control group (27250 ± 1284). There was significantly decreased wound area (Analysis of variance, p = 0.000) in the photobiomodulation + ADS (7.4 ± 1.4 mm(2)), ADS (11 ± 2.2 mm(2)), and photobiomodulation (11.4 ± 1.4 mm(2)) groups compared with the control group (25.2 ± 1.7). There was a significantly increased tensiometeric property (stress maximal load, Analysis of variance, p = 0.000) in the photobiomodulation + ADS (0.99 ± 0.06 N/cm(2)), ADS (0.51 ± 0.12 N/cm(2)), and photobiomodulation (0.35 ± 0.15 N/cm(2)) groups compared with the control group (0.18 ± 0.04). There was a significantly modulated inflammatory response in (Analysis of variance, p = 0.049) in the photobiomodulation + ADS (337 ± 96), ADS (1175 ± 640), and photobiomodulation (69 ± 54) treatments compared to control group (7321 ± 4099). Photobiomodulation + ADS gave significantly better improvements in CFU, wound area, and wound strength compared to photobiomodulation or ADS alone. Photobiomodulation, ADS, and their combination significantly hastened healing in ischemic methicillin-resistant Staphylococcus aureus infected delayed healing wounds in rats with DM2. Combined application of photobiomodulation plus ADS demonstrated an additive effect. Nature Publishing Group UK 2020-01-27 /pmc/articles/PMC6985227/ /pubmed/31988386 http://dx.doi.org/10.1038/s41598-020-58099-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Moradi, Ali
Zare, Fatemeh
Mostafavinia, Atarodsadat
Safaju, Sobhan
Shahbazi, Amirhossein
Habibi, Malihe
Abdollahifar, Mohammad-Amin
Hashemi, Seyed Mahmoud
Amini, Abdollah
Ghoreishi, Seyed Kamran
Chien, Sufan
Hamblin, Michael R.
Kouhkheil, Reza
Bayat, Mohammad
Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats
title Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats
title_full Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats
title_fullStr Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats
title_full_unstemmed Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats
title_short Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats
title_sort photobiomodulation plus adipose-derived stem cells improve healing of ischemic infected wounds in type 2 diabetic rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985227/
https://www.ncbi.nlm.nih.gov/pubmed/31988386
http://dx.doi.org/10.1038/s41598-020-58099-z
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