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Postnatal Expressional Patterns of Adipose-Associated Molecules in the Mouse Proximal Epididymal Fat
The epididymal fat pad is a male gonadal adipocyte tissue and is histochemically separated into distal and proximal parts. The development of epididymal fat during postnatal period has not been examined in detail. A previous research showed that expression of adipocyte-associated molecules in the di...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society of Developmental Biology
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985298/ https://www.ncbi.nlm.nih.gov/pubmed/31993537 http://dx.doi.org/10.12717/DR.2019.23.4.313 |
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author | Lee, Ki-Ho |
author_facet | Lee, Ki-Ho |
author_sort | Lee, Ki-Ho |
collection | PubMed |
description | The epididymal fat pad is a male gonadal adipocyte tissue and is histochemically separated into distal and proximal parts. The development of epididymal fat during postnatal period has not been examined in detail. A previous research showed that expression of adipocyte-associated molecules in the distal epididymal fat of mouse is generally increased as postnatally aged. In the present study, expressional patterns of same adipocyte-associated molecules in the mouse proximal epididymal fat at 2, 5, 8, and 12 months of age were studied by quantitative real-time PCR analysis and were compared with those in the distal epididymal fat. The expressional levels of peroxisome proliferator-activated receptor gamma (Pparg), lipoprotein lipase (Lpl), and fatty acid synthase (Fasn) at 5 months of age were significantly lower than those at 2 months of age, while transcript level of leptin (Lep) at 5 months was higher than that at 2 months of age. The transcript levels of all molecules at 8 months of age were significantly increased, compared with those at 2 and 5 months of age. At 12 months of age, expression of delta like non-canonical Notch ligand 1 (Dlk1) was further significantly increased, while there was no change on the transcript level of Pparg and significant decreases of Fabp4, Retn, Lpl, Lep, Fasn, and adiponectin (Adipoq) transcript levels. The current findings show that expressional patterns of molecules associated with adipocyte in the proximal epididymal fat is somewhat different with those of the distal epididymal fat, suggesting the existence of regional variance in the epididymal fat. |
format | Online Article Text |
id | pubmed-6985298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Korean Society of Developmental Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-69852982020-01-28 Postnatal Expressional Patterns of Adipose-Associated Molecules in the Mouse Proximal Epididymal Fat Lee, Ki-Ho Dev Reprod Original Research Paper The epididymal fat pad is a male gonadal adipocyte tissue and is histochemically separated into distal and proximal parts. The development of epididymal fat during postnatal period has not been examined in detail. A previous research showed that expression of adipocyte-associated molecules in the distal epididymal fat of mouse is generally increased as postnatally aged. In the present study, expressional patterns of same adipocyte-associated molecules in the mouse proximal epididymal fat at 2, 5, 8, and 12 months of age were studied by quantitative real-time PCR analysis and were compared with those in the distal epididymal fat. The expressional levels of peroxisome proliferator-activated receptor gamma (Pparg), lipoprotein lipase (Lpl), and fatty acid synthase (Fasn) at 5 months of age were significantly lower than those at 2 months of age, while transcript level of leptin (Lep) at 5 months was higher than that at 2 months of age. The transcript levels of all molecules at 8 months of age were significantly increased, compared with those at 2 and 5 months of age. At 12 months of age, expression of delta like non-canonical Notch ligand 1 (Dlk1) was further significantly increased, while there was no change on the transcript level of Pparg and significant decreases of Fabp4, Retn, Lpl, Lep, Fasn, and adiponectin (Adipoq) transcript levels. The current findings show that expressional patterns of molecules associated with adipocyte in the proximal epididymal fat is somewhat different with those of the distal epididymal fat, suggesting the existence of regional variance in the epididymal fat. Korean Society of Developmental Biology 2019-12 2019-12-31 /pmc/articles/PMC6985298/ /pubmed/31993537 http://dx.doi.org/10.12717/DR.2019.23.4.313 Text en © Copyright 2019 The Korean Society of Developmental Biology http://creative-commons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creative-commons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Paper Lee, Ki-Ho Postnatal Expressional Patterns of Adipose-Associated Molecules in the Mouse Proximal Epididymal Fat |
title | Postnatal Expressional Patterns of Adipose-Associated Molecules in
the Mouse Proximal Epididymal Fat |
title_full | Postnatal Expressional Patterns of Adipose-Associated Molecules in
the Mouse Proximal Epididymal Fat |
title_fullStr | Postnatal Expressional Patterns of Adipose-Associated Molecules in
the Mouse Proximal Epididymal Fat |
title_full_unstemmed | Postnatal Expressional Patterns of Adipose-Associated Molecules in
the Mouse Proximal Epididymal Fat |
title_short | Postnatal Expressional Patterns of Adipose-Associated Molecules in
the Mouse Proximal Epididymal Fat |
title_sort | postnatal expressional patterns of adipose-associated molecules in
the mouse proximal epididymal fat |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985298/ https://www.ncbi.nlm.nih.gov/pubmed/31993537 http://dx.doi.org/10.12717/DR.2019.23.4.313 |
work_keys_str_mv | AT leekiho postnatalexpressionalpatternsofadiposeassociatedmoleculesinthemouseproximalepididymalfat |