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DEPDC1B is a key regulator of myoblast proliferation in mouse and man
OBJECTIVES: DISHEVELLED, EGL‐10, PLECKSTRIN (DEP) domain‐containing 1B (DEPDC1B) promotes dismantling of focal adhesions and coordinates detachment events during cell cycle progression. DEPDC1B is overexpressed in several cancers with expression inversely correlated with patient survival. Here, we a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985657/ https://www.ncbi.nlm.nih.gov/pubmed/31825138 http://dx.doi.org/10.1111/cpr.12717 |
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author | Figeac, Nicolas Pruller, Johanna Hofer, Isabella Fortier, Mathieu Ortuste Quiroga, Huascar Pedro Banerji, Christopher R. S. Zammit, Peter S. |
author_facet | Figeac, Nicolas Pruller, Johanna Hofer, Isabella Fortier, Mathieu Ortuste Quiroga, Huascar Pedro Banerji, Christopher R. S. Zammit, Peter S. |
author_sort | Figeac, Nicolas |
collection | PubMed |
description | OBJECTIVES: DISHEVELLED, EGL‐10, PLECKSTRIN (DEP) domain‐containing 1B (DEPDC1B) promotes dismantling of focal adhesions and coordinates detachment events during cell cycle progression. DEPDC1B is overexpressed in several cancers with expression inversely correlated with patient survival. Here, we analysed the role of DEPDC1B in the regulation of murine and human skeletal myogenesis. MATERIALS AND METHODS: Expression dynamics of DEPDC1B were examined in murine and human myoblasts and rhabdomyosarcoma cells in vitro by RT‐qPCR and/or immunolabelling. DEPDC1B function was mainly tested via siRNA‐mediated gene knockdown. RESULTS: DEPDC1B was expressed in proliferating murine and human myoblasts, with expression then decreasing markedly during myogenic differentiation. SiRNA‐mediated knockdown of DEPDC1B reduced myoblast proliferation and induced entry into myogenic differentiation, with deregulation of key cell cycle regulators (cyclins, CDK, CDKi). DEPDC1B and β‐catenin co‐knockdown was unable to rescue proliferation in myoblasts, suggesting that DEPDC1B functions independently of canonical WNT signalling during myogenesis. DEPDC1B can also suppress RHOA activity in some cell types, but DEPDC1B and RHOA co‐knockdown actually had an additive effect by both further reducing proliferation and enhancing myogenic differentiation. DEPDC1B was expressed in human Rh30 rhabdomyosarcoma cells, where DEPDC1B or RHOA knockdown promoted myogenic differentiation, but without influencing proliferation. CONCLUSION: DEPDC1B plays a central role in myoblasts by driving proliferation and preventing precocious myogenic differentiation during skeletal myogenesis in both mouse and human. |
format | Online Article Text |
id | pubmed-6985657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69856572020-03-04 DEPDC1B is a key regulator of myoblast proliferation in mouse and man Figeac, Nicolas Pruller, Johanna Hofer, Isabella Fortier, Mathieu Ortuste Quiroga, Huascar Pedro Banerji, Christopher R. S. Zammit, Peter S. Cell Prolif Original Articles OBJECTIVES: DISHEVELLED, EGL‐10, PLECKSTRIN (DEP) domain‐containing 1B (DEPDC1B) promotes dismantling of focal adhesions and coordinates detachment events during cell cycle progression. DEPDC1B is overexpressed in several cancers with expression inversely correlated with patient survival. Here, we analysed the role of DEPDC1B in the regulation of murine and human skeletal myogenesis. MATERIALS AND METHODS: Expression dynamics of DEPDC1B were examined in murine and human myoblasts and rhabdomyosarcoma cells in vitro by RT‐qPCR and/or immunolabelling. DEPDC1B function was mainly tested via siRNA‐mediated gene knockdown. RESULTS: DEPDC1B was expressed in proliferating murine and human myoblasts, with expression then decreasing markedly during myogenic differentiation. SiRNA‐mediated knockdown of DEPDC1B reduced myoblast proliferation and induced entry into myogenic differentiation, with deregulation of key cell cycle regulators (cyclins, CDK, CDKi). DEPDC1B and β‐catenin co‐knockdown was unable to rescue proliferation in myoblasts, suggesting that DEPDC1B functions independently of canonical WNT signalling during myogenesis. DEPDC1B can also suppress RHOA activity in some cell types, but DEPDC1B and RHOA co‐knockdown actually had an additive effect by both further reducing proliferation and enhancing myogenic differentiation. DEPDC1B was expressed in human Rh30 rhabdomyosarcoma cells, where DEPDC1B or RHOA knockdown promoted myogenic differentiation, but without influencing proliferation. CONCLUSION: DEPDC1B plays a central role in myoblasts by driving proliferation and preventing precocious myogenic differentiation during skeletal myogenesis in both mouse and human. John Wiley and Sons Inc. 2019-12-11 /pmc/articles/PMC6985657/ /pubmed/31825138 http://dx.doi.org/10.1111/cpr.12717 Text en © 2019 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Figeac, Nicolas Pruller, Johanna Hofer, Isabella Fortier, Mathieu Ortuste Quiroga, Huascar Pedro Banerji, Christopher R. S. Zammit, Peter S. DEPDC1B is a key regulator of myoblast proliferation in mouse and man |
title | DEPDC1B is a key regulator of myoblast proliferation in mouse and man |
title_full | DEPDC1B is a key regulator of myoblast proliferation in mouse and man |
title_fullStr | DEPDC1B is a key regulator of myoblast proliferation in mouse and man |
title_full_unstemmed | DEPDC1B is a key regulator of myoblast proliferation in mouse and man |
title_short | DEPDC1B is a key regulator of myoblast proliferation in mouse and man |
title_sort | depdc1b is a key regulator of myoblast proliferation in mouse and man |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985657/ https://www.ncbi.nlm.nih.gov/pubmed/31825138 http://dx.doi.org/10.1111/cpr.12717 |
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