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Redox imaging and optical coherence tomography of the respiratory ciliated epithelium

Optical coherence tomography (OCT) is an emerging technology for in vivo airway and lung imaging. However, OCT lacks sensitivity to the metabolic changes caused by inflammation, which drives chronic respiratory diseases such as asthma and chronic obstructive pulmonary disorder. Redox imaging (RI) is...

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Autores principales: Gil, Daniel A., Sharick, Joe T., Mancha, Sophie, Gamm, Ute A., Choma, Michael A., Skala, Melissa C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985682/
https://www.ncbi.nlm.nih.gov/pubmed/30701725
http://dx.doi.org/10.1117/1.JBO.24.1.010501
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author Gil, Daniel A.
Sharick, Joe T.
Mancha, Sophie
Gamm, Ute A.
Choma, Michael A.
Skala, Melissa C.
author_facet Gil, Daniel A.
Sharick, Joe T.
Mancha, Sophie
Gamm, Ute A.
Choma, Michael A.
Skala, Melissa C.
author_sort Gil, Daniel A.
collection PubMed
description Optical coherence tomography (OCT) is an emerging technology for in vivo airway and lung imaging. However, OCT lacks sensitivity to the metabolic changes caused by inflammation, which drives chronic respiratory diseases such as asthma and chronic obstructive pulmonary disorder. Redox imaging (RI) is a label-free technique that uses the autofluorescence of the metabolic coenzymes NAD(P)H and flavin adenine dinucleotide (FAD) to probe cellular metabolism and could provide complimentary information to OCT for airway and lung imaging. We demonstrate OCT and RI of respiratory ciliated epithelial function in ex vivo mouse tracheae. We applied RI to measure cellular metabolism via the redox ratio [intensity of NAD(P)H divided by FAD] and particle tracking velocimetry OCT to quantify cilia-driven fluid flow. To model mitochondrial dysfunction, a key aspect of the inflammatory process, cyanide was used to inhibit oxidative metabolism and reduce ciliary motility. Cyanide exposure over 20 min significantly increased the redox ratio and reversed cilia-driven fluid flow. We propose that RI provides complementary information to OCT to assess inflammation in the airway and lungs.
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spelling pubmed-69856822020-02-03 Redox imaging and optical coherence tomography of the respiratory ciliated epithelium Gil, Daniel A. Sharick, Joe T. Mancha, Sophie Gamm, Ute A. Choma, Michael A. Skala, Melissa C. J Biomed Opt JBO Letters Optical coherence tomography (OCT) is an emerging technology for in vivo airway and lung imaging. However, OCT lacks sensitivity to the metabolic changes caused by inflammation, which drives chronic respiratory diseases such as asthma and chronic obstructive pulmonary disorder. Redox imaging (RI) is a label-free technique that uses the autofluorescence of the metabolic coenzymes NAD(P)H and flavin adenine dinucleotide (FAD) to probe cellular metabolism and could provide complimentary information to OCT for airway and lung imaging. We demonstrate OCT and RI of respiratory ciliated epithelial function in ex vivo mouse tracheae. We applied RI to measure cellular metabolism via the redox ratio [intensity of NAD(P)H divided by FAD] and particle tracking velocimetry OCT to quantify cilia-driven fluid flow. To model mitochondrial dysfunction, a key aspect of the inflammatory process, cyanide was used to inhibit oxidative metabolism and reduce ciliary motility. Cyanide exposure over 20 min significantly increased the redox ratio and reversed cilia-driven fluid flow. We propose that RI provides complementary information to OCT to assess inflammation in the airway and lungs. Society of Photo-Optical Instrumentation Engineers 2019-01-30 2019-01 /pmc/articles/PMC6985682/ /pubmed/30701725 http://dx.doi.org/10.1117/1.JBO.24.1.010501 Text en © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle JBO Letters
Gil, Daniel A.
Sharick, Joe T.
Mancha, Sophie
Gamm, Ute A.
Choma, Michael A.
Skala, Melissa C.
Redox imaging and optical coherence tomography of the respiratory ciliated epithelium
title Redox imaging and optical coherence tomography of the respiratory ciliated epithelium
title_full Redox imaging and optical coherence tomography of the respiratory ciliated epithelium
title_fullStr Redox imaging and optical coherence tomography of the respiratory ciliated epithelium
title_full_unstemmed Redox imaging and optical coherence tomography of the respiratory ciliated epithelium
title_short Redox imaging and optical coherence tomography of the respiratory ciliated epithelium
title_sort redox imaging and optical coherence tomography of the respiratory ciliated epithelium
topic JBO Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985682/
https://www.ncbi.nlm.nih.gov/pubmed/30701725
http://dx.doi.org/10.1117/1.JBO.24.1.010501
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